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  • 1
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    Visualizing calcium flux in freely moving nematode embryos (2017)
    Details
    Publication Date: 2022-05-25
    Description: Author Posting. © The Author(s), 2017. This is the author's version of the work. It is posted here by permission of Cell Press for personal use, not for redistribution. The definitive version was published in Biophysical Journal 112 (2017): 1975-1983, doi:10.1016/j.bpj.2017.02.035.
    Description: The lack of physiological recordings from Caenorhabditis elegans embryos stands in stark contrast to the comprehensive anatomical and gene expression datasets already available. Using light-sheet fluorescence microscopy (LSFM) to address the challenges associated with functional imaging at this developmental stage, we recorded calcium dynamics in muscles and neurons and developed analysis strategies to relate activity and movement. In muscles, we found that the initiation of twitching was associated with a spreading calcium wave in a dorsal muscle bundle. Correlated activity in muscle bundles was linked with early twitching and eventual coordinated movement. To identify neuronal correlates of behavior, we monitored brain-wide activity with subcellular resolution and identified a particularly active cell associated with muscle contractions. Finally, imaging neurons of a well-defined adult motor circuit, we found that reversals in the eggshell correlated with calcium transients in AVA interneurons.
    Description: E.A. and A.K. acknowledge support from the Grass Fellowship Program and D. C-R. and H.S. acknowledge the Whitman Fellowship program at MBL. This work was supported by the intramural research program of the National Institute of Biomedical Imaging and Bioengineering and NIH grants U01 HD075602 and R24OD016474 to D.C-R and A.K.
    Description: 2018-05-09
    Repository Name: Woods Hole Open Access Server
    Type: Preprint
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  • 2
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    Aligning Standards Communities: Sustainable Darwin Core MIxS Interoperability (2021)
    In:  EPIC3Biodiversity Information Science and Standards, 5(e73775)
    Details
    Publication Date: 2021-10-04
    Repository Name: EPIC Alfred Wegener Institut
    Type: Article , peerRev
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  • 3
    Electronic Resource
    Electronic Resource
    Alloimmune reactions among recently wild syrian hamsters and classical inbred strains include alloantibody production (1979)
    Springer
    Immunogenetics 9 (1979), S. 563-573 
    Details
    ISSN: 1432-1211
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Partially inbred lines of Syrian hamsters, the descendants of animals caught wild in Syria within the past decade, have been studied to determine their immunogenetic relationships to the long established domestic inbred strains. These new sources of hamster genes display strong alloimmune reactions when confronted with tissues from the domestic inbred strains and vice versa: acute skin graft rejection, strong mixed lymphocyte reactions, and intense graft-versus-host reactions. While these forms of reactivity have also characterized the disparities among the domestic inbred strains, no evidence of alloantibodies specific for putative transplantation antigens has ever been found in hamsters. We report, for the first time, that immunization of recently wild hamsters with tissues from domestic inbred strain donors elicits high titer cytotoxic antibodies directed at alloantigens. Similarly, animals of the domestic inbred strains produce comparably strong alloantibody responses when immunized with tissues from the recently wild hamsters. Availability of these alloantisera will make the possibility of defining the major histocompatibility complex equivalent in this species much more likely.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01570450
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  • 4
    Electronic Resource
    Electronic Resource
    Book review (1978)
    Springer
    Immunogenetics 6 (1978), S. 403-403 
    Details
    ISSN: 1432-1211
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01563932
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  • 5
    Electronic Resource
    Electronic Resource
    The biochemical characterization of Syrian hamster cell-surface alloantigens (1981)
    Springer
    Immunogenetics 12 (1981), S. 485-496 
    Details
    ISSN: 1432-1211
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Immunochemical comparisons between hamster cell-surface p39/p29 alloantigens and putative MHC class I and class II products indicate that p39/p29 alloantigens are probable class II products. Both p39/p29 alloantigens and class II homologues have varying expression in hamster lymphoid tissues and are absent from fibroblasts. In addition, analysis by nonequilibrium pH gradient electrophoresis demonstrates similar, although nonidentical, alloantigen and class II homologous charge profiles. This observation could reflect multiple class II subregions within the hamster MHC. These data therefore suggest an immunochemical description of the hamster MHC which is compatible with MHC models proposed for other mammalian species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01561690
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  • 6
    Electronic Resource
    Electronic Resource
    Genetic analyses of alloreactions between recently wild and classical inbred strains of syrian hamsters: Evidence in favor of a major histocompatibility complex (1981)
    Springer
    Immunogenetics 13 (1981), S. 393-403 
    Details
    ISSN: 1432-1211
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Cytotoxic alloantisera were raised between recently wild and classical inbred strains of Syrian hamsters. Antisera produced by immunizing the classical inbred strains with tissue from the partially inbred, recently wild hamsters detect several specificities shared between the classical and recently wild strains. Reciprocal mixed lymphocyte reactions between the two different groups of hamsters suggest that the new source of hamsters possesses several unique MLR phenotypes which may represent new Hm-1 haplotypes. Moreover, several recently wild strains express MLR phenotypes quite similar if not identical to the Hm-1 a haplotype of the inbred strain, MHA. Genetic analyses of alloreactions between domestic inbred and recently wild strains suggest that a single locus or chromosomal region encodes the allodeterminants that induce strong MLR reactivity. Six unique MLR phenotypes have been defined which most likely represent haplotypes of the hamster MHC equivalent, Hm-1. Genetic linkage studies indicate that some alloantisera detect determinants encoded by loci closely linked to the MLR locus, and therefore define Hm-1 determinants. Moreover, other alloantisera recognize determinants encoded by a locus that is unlinked to Hm-1. These studies suggest that Syrian hamsters express a polymorphic MHC equivalent, Hm-1, which encodes determinants that induce both cell-mediated and humoral alloreactivity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00346020
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  • 7
    Electronic Resource
    Electronic Resource
    Syrian hamster DNA shows limited polymorphism at class I-like loci (1985)
    Springer
    Immunogenetics 22 (1985), S. 257-268 
    Details
    ISSN: 1432-1211
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The class I gene products of the Syrian hamster major histocompatibility complex are unique in that they lack functionally detectable polymorphism. Mouse cDNA and hamster genomic probes were used to analyze the hamster class I gene family using genomic Southern hybridization. These studies revealed that the hamster possesses a complex class I multigene family and that it shares extensive sequence homology with the corresponding mouse sequences. Unlike the mouse, however, the Syrian hamster demonstrates only limited restriction endonuclease polymorphism in these genes. These results suggest that the lack of detectable polymorphism in this species is directly related to limited DNA polymorphism. The data presented here support the hypothesis that this species has undergone an evolutionary bottleneck, i. e., that all surviving members of the species arose from a limited number of progenitors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00404485
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  • 8
    Electronic Resource
    Electronic Resource
    Detection of two distinct class II α:β:Ii complexes in the syrian hamster (1982)
    Springer
    Immunogenetics 16 (1982), S. 425-433 
    Details
    ISSN: 1432-1211
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Hamster alloantisera and a monoclonal antibody originally generated against antigens controlled by the murine I-E k subregion, which cross-reacts with hamster cell surface antigens, have been used to define two distinct Ia-like complexes in the Syrian hamster. These complexes have been named α1: β 1 and α2:β 2 and are detected by hamster alloantisera or monoclonal antibody 14-4-4, respectively. For the three strains studied, α1:β 1, appears to be polymorphic in both α and β chains, while the α2:β 2 complex is nonpolymorphic, as revealed by 2-D PAGE analyses. A third nonpolymorphic glycoprotein that appears to be the hamster's equivalent of the murine invariant chain (Ii) is associated with both the α1:β 1 and α2:β 2 complexes. In addition, we report the first biochemical detection of polymorphism between the closely related CB and MHA Syrian hamster strains.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00372101
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  • 9
    Electronic Resource
    Electronic Resource
    The Biochemical characterization of syrian hamster cell-surface alloantigen (1978)
    Springer
    Immunogenetics 7 (1978), S. 445-455 
    Details
    ISSN: 1432-1211
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The first alloantiserum to be described in Syrian hamsters has been characterized for its ability to react with externally and internally radiolabeled antigens derived from normal hamster lymphoid cells. Utilizing conventional biochemical techniques, radioiodinated and3H-leucine labeled cellular extracts have been prepared, partially purified by lentil lectin affinity chromatography, and indirectly immunoprecipitated with experimental alloantisera. Analysis of the precipitated radiolabeled antigens by polyacrylamide gel electrophoresis in SDS has identified two prominent cell-surface proteins at 39,000 (p39) and 29,000 daltons (p29) on 2-mercaptoethanol reduced gels. Further analysis of the radiolabeled extract has demonstrated the existence of hamster cell-surface proteins at 43,000 and 12,000 daltons which are immunoprecipitated by a xenoantiserum directed against human β2 microglobulin. Coelectrophoretic studies indicate the independent identity of these four species of hamster cellsurface proteins. These results suggest that between two hamster lines, derived from animals caught 40 years apart from different geographic locations in Syria, polymorphism of cell-surface antigens is restricted to p39 and p29 molecular species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01844034
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  • 10
    Electronic Resource
    Electronic Resource
    Immunochemical detection of hamster class II MHC homologues by murine anti-Iak sera (1979)
    Springer
    Immunogenetics 9 (1979), S. 477-486 
    Details
    ISSN: 1432-1211
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Syrian hamster la-like cell-surface glycoproteins have been identified by immunoprecipitation of radiolabeled hamster lymphocyte lysates with cross-reacting murine anti-Iak alloantisera. Two components of 39 000 and 29 000 daltons were discerned by polyacrylamide gel electrophoresis in SDS (SDS-PAGE). Absorption of the antisera with mouse or hamster lymphoid cells demonstrated the cross-reactive anti-Iak antibodies to be specific for hamster Ia homologues. These molecules were also shown to have radiolabeling characteristics similar to Ia antigens of the mouse and guinea pig. These studies suggest that distinct structural similarities exist between murine Ia and the hamster Ia-like molecules described here. In view of our previous immunochemical description of putative hamster class I homologues, the current identification of hamster class II homologues suggests that an adequate and contemporary description of the hamster major histocompatibility complex is possible and likely.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01570440
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