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  • 1
    Electronic Resource
    Electronic Resource
    Detection and identification of gibberellins in Douglas fir (Pseudotsuga menziesii) shoots (1992)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 85 (1992), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Extracts of Douglas fir (Pseudotsuga menziesii [Mirb.] Franco) shoots were purified by reversed and normal phase HPLC; gibberellin (GA)-like compounds detected by radioimmunoassay with antibodies against GA4 and the Tan-ginbozu dwarf rice micro-drop biossay were analyzed by GC-MS. Three major components were identified as GA4, GA7, and GA9 while smaller amounts of GA1, GA3 and putative GA9-glucosyl ester were also present.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1992.tb05816.x
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  • 2
    Electronic Resource
    Electronic Resource
    Temporal responses of Arabidopsis root architecture to phosphate starvation: evidence for the involvement of auxin signalling (2003)
    Oxford, UK : Blackwell Science Ltd
    Plant, cell & environment 26 (2003), S. 0 
    Details
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The ability of the root system architecture to respond to nutrient availability is a key adaptative behaviour allowing plants to cope with environmental conditions. On the basis of single time point comparisons, the response to phosphate deprivation was previously shown to involve both the primary and lateral roots of Arabidopsis. In this work, the temporal pattern of Arabidopsis root responses to phosphate starvation was investigated. Daily scanning of roots showed that changes in architecture were largely due to the alterations of time-based growth parameters, namely a decrease in the elongation rate of the primary root opposed to an increase in the elongation rate of lateral roots and a decrease in the number of initiated lateral roots. In addition, another identified response was a decrease in the proportion of lateral roots showing early growth arrest. All these changes occurred within a short period of approximately 3 d. In addition, the root morphology comparison with the auxin-resistant mutant axr4, the auxin-treatment of phosphate-starved plants and a limited transcriptome analysis supported the conclusion that auxin signalling was involved in the adaptive response of the root system architecture to phosphate deprivation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-3040.2003.01030.x
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  • 3
    Electronic Resource
    Electronic Resource
    Effects of phosphate availability on the root system architecture: large-scale analysis of the natural variation between Arabidopsis accessions (2003)
    Oxford, UK : Blackwell Science Ltd
    Plant, cell & environment 26 (2003), S. 0 
    Details
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Developmental plasticity is one main adaptative response of plants to the availability of nutrients. In the present study, the naturally occurring variation existing in Arabidopsis for the growth responses to phosphate availability was investigated. Initially details of the effects of phosphate starvation for the four currently used accessions Cvi, Col, Ler and Ws were compared. A set of 10 growth parameters, concerning the aerial part and the root system, was measured in both single-point and time-course experiments. The length of the primary root and the number of laterals were found to be consistently reduced by phosphate starvation in all four accessions. These two robust parameters were selected to further screen a set of 73 accessions originating from a wide range of habitats. One-half of the accessions showed also a reduced primary root and less lateral roots when phosphate-starved, and 25% were not responsive to phosphate availability. For the last quarter of accessions, phosphate starvation was found to affect only one of the two growth parameters, indicating the occurrence of different adaptative strategies. These accessions appear to offer new tools to investigate the molecular basis of the corresponding growth responses to phosphate availability.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-3040.2003.01100.x
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  • 4
    Electronic Resource
    Electronic Resource
    Transcription factor genes with expression correlated to nitrate-related root plasticity of Arabidopsis thaliana (2003)
    Oxford, UK : Blackwell Publishing Ltd
    Plant, cell & environment 26 (2003), S. 0 
    Details
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The root response to changes in nitrogen availability was examined in Arabidopsis thaliana. Plants grown on vertically oriented Petri plates with nutrient media containing either 10 or 0.5 mm KNO3 supplemented with 5 mm glutamine were analysed for changes in root architecture, morphology and nitrogen status compared to plants grown on 0.5 mm KNO3. Fourteen-day-old 10 mm KNO3-grown plants displayed a decrease in number, length and density of lateral roots, had the highest tissue NO3– content, but no change in amino acid composition. Lateral root development was arrested at a stage after the initiation of primordia but prior to emergence. In contrast, plants grown on 0.5 mm KNO3 supplemented with glutamine had a slightly higher number and density of lateral root primordia and lateral roots, lower root NO3– content, and higher tissue glutamine content. A macro-array containing 126 putative A. thaliana transcriptional factor expressed sequence tags was used to screen for regulatory genes with expression correlated to the root plasticity response. Genes for a basic leucine zipper (bZIP) and a LIM transcription factor were identified and via Northern analysis it was confirmed that their expression is preferentially observed in the roots and correlated to the root response to nitrate availability.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-3040.2003.00977.x
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  • 5
    Electronic Resource
    Electronic Resource
    Effect of autoclaving amino acids on in vitro rooting response of wild cherry shoot
    Amsterdam : Elsevier
    Scientia Horticulturae 57 (1994), S. 89-98 
    Details
    ISSN: 0304-4238
    Keywords: Amino acids ; Prunus avium ; Rooting ; Tissue culture
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0304-4238(94)90037-X
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  • 6
    Electronic Resource
    Electronic Resource
    Histological investigation of walnut cotyledon fragments for a better understanding of in vitro adventitious root initiation (1996)
    Springer
    Plant cell reports 15 (1996), S. 345-349 
    Details
    ISSN: 1432-203X
    Keywords: Walnut cotyledon ; Adventitious root formation ; Tissue culture ; Histological investigation ; Auxin treatment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Rapid formation of adventitious roots by walnut cotyledon fragments in vitro was traced by light microscopy. It was shown that this plant model is characterized by two major developmental processes: a) confined elongation of the cotyledon petiole caused by a limited number of cell divisions and b) formation of a morphogenetic zone around each initially wounded vascular bundle within 36 h after detachment of the embryonic axis. During the first phase of development, granular storage protein bodies dissolved, and starch grains were deposited mainly in the distal portion of the cotyledon fragments. Rapidly, new globular protein bodies were formed, and phenolic inclusions accumulated in the vacuoles of epidermal and subepidermal cells and of individual cells close to the vascular bundles. Each adventitious root was found to be in continuity with a single vascular bundle of the cotyledon petiole. A short auxin treatment suppressed the formation of large roots and induced numerous tiny rootlets dispersed all over the surface of the cotyledons.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232369
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  • 7
    Electronic Resource
    Electronic Resource
    Histological investigation of walnut cotyledon fragments for a better understanding of in vitro adventitious root initiation (1996)
    Springer
    Plant cell reports 15 (1996), S. 345-349 
    Details
    ISSN: 1432-203X
    Keywords: Key words: Walnut cotyledon – Adventitious root formation – Tissue culture – Histological investigation – Auxin treatment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary. Rapid formation of adventitious roots by walnut cotyledon fragments in vitro was traced by light microscopy. It was shown that this plant model is characterized by two major develomental processes: a) confined elongation of the cotyledon petiole caused by a limited number of cell divisions and b) formation of a morphogenetic zone around each initially wounded vascular bundle within 36 h after detachment of the embryonic axis. During the first phase of development, granular storage protein bodies dissolved, and starch grains were deposited mainly in the distal portion of the cotyledon fragments. Rapidly, new globular protein bodies were formed, and phenolic inclusions accumulated in the vacuoles of epidermal and subepidermal cells and of individual cells close to the vascular bundles. Each adventitious root was found to be in continuity with a single vascular bundle of the cotyledon petiole. A short auxin treatment suppressed the formation of large roots and induced numerous tiny rootlets dispersed all over the surface of the cotyledons.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050030
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  • 8
    Electronic Resource
    Electronic Resource
    Development of a one-step enzyme immunoassay for the quantitation of gibberellin A3 in barley seeds (1995)
    Springer
    Plant growth regulation 17 (1995), S. 7-13 
    Details
    ISSN: 1573-5087
    Keywords: polyclonal antibodies ; enzyme immunoassay ; GA3 ; GC-MS
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The development of a sensitive and specific enzyme immunoassay for GA3 is reported. This method was based on the use of peroxidase labelled GA3 and immobilized antibodies. In order to obtain a rapid immunoassay, several steps of purification were analyzed to show their necessity. Barley seed extracts were assayed at different steps of purification to exhibit the effect of extract components on the assay. It was demonstrated that HPLC had to be performed when a selective quantitation of GA3 was required. This assay allowed GA3 to be measured with reproducibility as its unmethylated form and the quantitation of GA3 in barley seeds with this enzyme immunoassay was correlated to a GC-MS method.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00024488
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  • 9
    Electronic Resource
    Electronic Resource
    Distribution of endogenous gibberellins in dwarf and giant off-types banana (Musa AAA, cv.‘Grand nain’) plants from in vitro propagation (1995)
    Springer
    Plant growth regulation 17 (1995), S. 219-224 
    Details
    ISSN: 1573-5087
    Keywords: Gibberellin ; in vitro propagation ; Musa, quantification ; somaclonal variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract GA3 and GA20 were quantified in leaf extracts from true-to-type and somaclonal variants (dwarf and giant) of Musa AAA cv. ‘Grand nain’ by GC-MS-SIM after purification on reverse- and normal-phase HPLC and detection by ELISA with GA3 antibodies and by a dwarf rice bioassay. GA3 concentration in dwarf plants was 811 ng g−1 dry weight. For normal and giant plants, the endogeneous GA3 levels were respectively 3.6 and 4.6 times higher. The GA20 concentration in the giant plant was 68 ng g−1 of dry weight. This concentration was, respectively, 4.6 and 7.3 times higher than those of normal and dwarf plants. These results suggest that the somaclonal variations affecting banana plant height are associated with modifications in GA metabolism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00024729
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  • 10
    Electronic Resource
    Electronic Resource
    Quantification of GA1, GA3, GA4, GA7, GA8, GA9, GA19 and GA20; and GA20 metabolism in dormant and non-dormant beechnuts (1997)
    Springer
    Plant growth regulation 22 (1997), S. 29-35 
    Details
    ISSN: 1573-5087
    Keywords: beechnut ; dormancy breaking ; Fagus sylvatica ; gibberellin ; GC-MS-SIM ; seed
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The endogenous levels of GA1, GA3, GA4, GA7, GA8, GA9, GA19 and GA20 were determined in beech seeds (Fagus sylvatica L.) treated with different dormancy breaking treatments. Gibberellins were analysed separately in cotyledons and embryo axes. After purification of the extracts, GAs were quantified by GC-MS-selected ion monitoring (GC-MS-SIM) with deuterated GAs as internal standards. The results showed that GAs corresponding to the 13-OH pathway seemed to be involved in dormancy breaking. Strong differences in GA1, GA3, GA8, GA19 and GA20 levels between embryo axes and cotyledons of dormant and non-dormant beechnuts were detected with less pronounced differences for GA4, GA7 and GA9 levels. Both the quantitative differences between dormant and non-dormant seeds in the analysed GAs corresponding to the 13-OH pathway, and the capacity of non-dormant seeds to carry out metabolic conversions when labelled GA20 was injected into the seeds, reveal a dynamic role of GAs in dormancy release.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005814926549
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