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Distribution of endogenous gibberellins in dwarf and giant off-types banana (Musa AAA, cv.‘Grand nain’) plants from in vitro propagation

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Abstract

GA3 and GA20 were quantified in leaf extracts from true-to-type and somaclonal variants (dwarf and giant) of Musa AAA cv. ‘Grand nain’ by GC-MS-SIM after purification on reverse- and normal-phase HPLC and detection by ELISA with GA3 antibodies and by a dwarf rice bioassay. GA3 concentration in dwarf plants was 811 ng g−1 dry weight. For normal and giant plants, the endogeneous GA3 levels were respectively 3.6 and 4.6 times higher. The GA20 concentration in the giant plant was 68 ng g−1 of dry weight. This concentration was, respectively, 4.6 and 7.3 times higher than those of normal and dwarf plants. These results suggest that the somaclonal variations affecting banana plant height are associated with modifications in GA metabolism.

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Abbreviations

HPLC:

High Performance Liquid Chromatography

GC-MS:

Gas Chromatography-Mass Spectrometry

SIM:

Selected Ion Monitoring

GA:

Gibberellin

BSA:

Bovine Serum Albumin

PB:

Phosphate Buffer

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Authors and Affiliations

  1. Laboratoire BIOTROP, CIRAD-FLHOR, BP. 5035, 34032, Montpellier, France

    J. Sandoval, F. Kerbellec & F. Côte

  2. INRA, Centre de Recherches d'Orléans, Station d'Amélioration des Arbres Forestiers, 45160, Ardon, France

    P. Doumas

Authors
  1. J. Sandoval
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  2. F. Kerbellec
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  3. F. Côte
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  4. P. Doumas
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Sandoval, J., Kerbellec, F., Côte, F. et al. Distribution of endogenous gibberellins in dwarf and giant off-types banana (Musa AAA, cv.‘Grand nain’) plants from in vitro propagation. Plant Growth Regul 17, 219–224 (1995). https://doi.org/10.1007/BF00024729

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  • DOI: https://doi.org/10.1007/BF00024729

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