ISSN:
1432-2048
Keywords:
Key words:Chlamydomonas
;
Osmotic stress
;
Phosphatidylinositol 3
;
5-bisphosphate
;
Signal transduction
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract. Cells from several different plant species synthesised a polyphosphoinositide (PPI)-like lipid when osmo-stressed. Synthesis was maximal after about 10 min and was stimulated by a variety of osmolytes. Using NaCl, the strongest response centred around 200 mM. The lipid was shown to be the novel PPI isomer phosphatidyl-inositol 3,5-bisphosphate [PtdIns-(3,5)P2] by analytical thin-layer chromatography and conversion to PtdIns(3,4,5)P3 using recombinant phosphoinositide 4-OH kinase. The results indicate that PtdIns-(3,5)P2 plays a role in a general osmo-signalling pathway in plants. Its potential role is discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s004250050561
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