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Hyperosmotic stress induces rapid synthesis of phosphatidyl-D-inositol 3,5-bisphosphate in plant cells

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Abstract.

Cells from several different plant species synthesised a polyphosphoinositide (PPI)-like lipid when osmo-stressed. Synthesis was maximal after about 10 min and was stimulated by a variety of osmolytes. Using NaCl, the strongest response centred around 200 mM. The lipid was shown to be the novel PPI isomer phosphatidyl-inositol 3,5-bisphosphate [PtdIns-(3,5)P2] by analytical thin-layer chromatography and conversion to PtdIns(3,4,5)P3 using recombinant phosphoinositide 4-OH kinase. The results indicate that PtdIns-(3,5)P2 plays a role in a general osmo-signalling pathway in plants. Its potential role is discussed.

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Authors and Affiliations

  1. Institute of Molecular Cell Biology, Biocentrum, University of Amsterdam, Kruislaan 318, NL-1098 SM Amsterdam, The Netherlands, , , , , , NL

    Harold J. G. Meijer, Herman van den Ende, Alan Musgrave & Teun Munnik

  2. Division of Cellular Biochemistry, The Netherlands Cancer Institute, Plesmanlaan 121, NL-1066 CX, Amsterdam, The Netherlands, , , , , , NL

    Nullin Divecha

Authors
  1. Harold J. G. Meijer
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  2. Nullin Divecha
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  3. Herman van den Ende
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  4. Alan Musgrave
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  5. Teun Munnik
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Received: 6 November 1998 / Accepted: 14 December 1998

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Meijer, H., Divecha, N., van den Ende, H. et al. Hyperosmotic stress induces rapid synthesis of phosphatidyl-D-inositol 3,5-bisphosphate in plant cells. Planta 208, 294–298 (1999). https://doi.org/10.1007/s004250050561

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  • DOI: https://doi.org/10.1007/s004250050561

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