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  • 1
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 31 (1988), S. 958-968 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The influence of operational, parameters, such as hydraulic retention time, organic loading rate, influent substrate concentration, pH, and temperature, on the performance of the first phase of anaerobic digestion has been investigated. A complex substrate based on beef extract was used, and six series of experimental runs were conducted, each one showing the effect of one operational variable. The predominant fermentation products were always acetic and propionic acid, independent of the values of the operational parameters. For initial COD concentrations and hydraulic retention times above the critical values identified as 3 g/L and 6 h, respectively, the degree of acidification achieved was between 30 and 60%. The degree of acidification was found to increase with the hydraulic retention time and decrease with the influent substrate concentration and organic loading rate, while the opposite held true for the rate of product formation. Furthermore, it has been demonstrated that acidification is primarily determined by the hydraulic retention time and the rate of product formation by the influent substrate concentration. The concentration of the acetic acid produced was found to depend on the operational parameters. However, the concentration of propionic acid produced depended only on the substrate availability with a consistent proportion of 8% initial COD converted to it. The optimum pH and temperature were 7 and 40°C, respectively. The percentage of acetic acid as a proportion of the total volatile fatty acids produced was found to increase with increasing pH and temperature, while the percentage of propionic acid seemed to decrease accordingly. Finally the effect of the temperature on the rate of acidification followed an Arrhenius type equation with an activation energy equal to 4739 cal/mol.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 31 (1988), S. 969-978 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The kinetics of the acidogenic phase of anaerobic digestion were studied in continuous culture using a complex substrate based on beef extract. A number of different steady states was achieved covering a wide range of hydraulic retention times and product concentrations. The Monod equation failed completely to describe the growth of the acidogenic bacteria, while there was evidence of product inhibition. Therefore, two of the most commonly used inhibition models were applied, the non-competitive and the Haldane-type model. The former gave superior results, and the estimated values for the biokinetic constants were a maximum specific growth rate (μmax) of 32.6 day-1, a substrate saturation constant (Ks) of 1.77 g COD/L, an inhibition constant (Ki) of 0.633 g/L of total volatile fatty acids, and a specific decay rate (kd) 1.747 day-1. The same inhibition models were also applied to the description of the specific rate of product formation, with the noncompetitive model fitting the data slightly better. The maximum specific rate of product formation was estimated to be 125.7 g total volatile fatty acid/g biomass/day.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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