ALBERT — All Library Books, journals and Electronic Records Telegrafenberg

1

Electronic Resource

Amino acids and bivalent cations in the growth of tobacco pollen in mass culture

Tupy, J. ; Hrabetova, E. ; Capkova, V.

Amsterdam : Elsevier

Plant Science Letters 30 (1983), S. 91-98

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ISSN: 0304-4211

Keywords: Amino acids ; Nicotiana tabacum L. ; Pollen culture

Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Topics: Biology

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1016/0304-4211(83)90207-9

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2

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Sites of actin filament initiation and reorganization in cold-treated tobacco cells (2004)

POKORNÁ, J. ; SCHWARZEROVÁ, K. ; ZELENKOVÁ, S. ; [et al.]

Oxford, UK : Blackwell Science Ltd

Plant, cell & environment 27 (2004), S. 0

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ISSN: 1365-3040

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Biology

Notes: Cytoskeletal proteins assemble into dynamic polymers that play many roles in nuclear and cell division, signal transduction, and determination of cell shape and polarity. The distribution and dynamics of microtubules (MTs) and actin filaments (AFs) are determined, among other factors, by the location of their nucleation sites. Whereas the sites of microtubule nucleation in plants are known to be located under the plasma membrane and on the nuclear envelope during interphase, there is a striking lack of information about nucleation sites of AFs. In the studies reported herein, low temperature (0 °C) was used to de-polymerize AFs and MTs in tobacco BY-2 (Nicotiana tabacum L.) cells at interphase. The extent of de-polymerization of cytoskeletal filaments in interphase cells during cold treatment and the subcellular distribution of nucleation sites during subsequent recovery at 25 °C were monitored by means of fluorescence microscopy. The results show that AFs re-polymerized rapidly from sites located in the cortical region and on the nuclear envelope, similarly to the initiation sites of MTs. In contrast to MTs, however, complete reconstitution of AFs was preceded by the formation of transient actin structures including actin dots, rods, and filaments with a dotted signal. Immunoblotting of soluble and sedimentable protein fractions showed no changes in the relative amounts of free and membrane-bound actin or tubulin.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.1365-3040.2004.01186.x

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3

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Culture on sugar medium enhances photosynthetic capacity and high light resistance of plantlets grown in vitro (1998)

Tichá, I. ; Čáp, F. ; Pacovská, D. ; [et al.]

Copenhagen : Munksgaard International Publishers

Physiologia plantarum 102 (1998), S. 0

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ISSN: 1399-3054

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Biology

Notes: The significance of photosynthetic photon flux (PPF) and sugar feeding for the production of plants in vitro is only poorly understood. Nicotiana tabacum L. plantlets were grown photoautotrophically and photomixotrophically (3% sucrose) at two different PPFs (60 µmol m−2 s−1 and 200 µmol m−2 s−1) to investigate the effect of these culture parameters on photosynthetic performance and growth. Photomixotrophically-grown plantlets showed an increase in carbohydrate content, mainly in glucose and fructose. Plant growth, dry matter accumulation and total leaf area were higher under photomixotrophic than photoautotrophic conditions. Not only biomass formation but also photosynthesis was positively affected by exogenous sucrose; the chlorophyll (Chl) content and the light-saturated rate of photosynthetic oxygen evolution were higher in photomixotrophic plantlets. Photoinhibition occurred in plantlets that were grown photoautotrophically at the higher PPF. It became apparent as a loss in Chl content and photochemical efficiency. Photoinhibited plantlets showed a decrease in the D2/LHCII and CP47/LHCII ratios, suggesting a preferential loss of proteins from the photosystem II (PSII) core. The increased content of xanthophyll cycle pigments in photoinhibited plantlets indicated that also protective mechanisms were activated. Photomixotrophic growth of the plantlets prevented the occurrence of photoinhibitory symptoms. Therefore, we conclude that culture on sugar medium increases not only the photosynthetic potential but also the high light resistance of plantlets grown in vitro.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1034/j.1399-3054.1998.1020201.x

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4

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Temporal Changes in the RNA Distribution between Polysomes and Postpolysomal Ribonucleoprotein Particles in Tobacco Male Gametophyte (2000)

Honys, D. ; Čapková, V.

Springer

Biologia plantarum 43 (2000), S. 517-522

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ISSN: 1573-8264

Keywords: in vitro translation ; Nicotiana tabacum L. ; pollen ; polysomes ; stored mRNA ; translational regulation

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract Growth of tobacco (Nicotiana tabacum L.) pollen tube is controlled by post-transcriptionally regulated protein synthesis. Stored mRNA was found to be present in the form of messenger ribonucleoprotein particles (mRNPs) in both maturing and germinating pollen. During pollen dehydration in the anthers content of mRNPs strongly increased during dissociation of polysomes suggesting a transfer of mRNA liberated from polysomal structures into mRNP particles. Pollen germination and initial pollen tube growth characterized by rapid reassociation of ribosomes was accompanied by decrease of mRNPs indicating an involvement of mRNA of these particles in the formation of polysomes. Distribution of the particular mRNAs during the pollen activation preceeding tube growth suggests that other mechanisms along with pollen rehydration are engaged in this process and that the continuous exploitation of stored mRNPs during pollen tube growth is precisely regulated.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1023/A:1002846105299

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5

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Protein synthesis in tobacco pollen tubes: preferential synthesis of cell-wall 69-kDa and 66-kDa glycoproteins (1994)

Čapková, V. ; Tupý, J. ; Zbrožek, J.

Springer

Sexual plant reproduction 7 (1994), S. 57-66

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ISSN: 1432-2145

Keywords: Nicotiana tabacum ; Pollen tube growth Protein synthesis ; Wall bound proteins Glycoproteins ; Tunicamycin

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract One- and two-dimensional electrophoresis of Nicotiana tabacum pollen and pollen tube proteins confirmed that a new protein is preferentially synthesized during pollen germination and tube growth and becomes the most abundant protein in pollen tubes. Analysis of proteins extracted with sodium dodecyl sulfate (SDS) from different pollen tube fractions showed that it is the most abundant non-covalently bound wall protein, characterized by molecular mass of 69 kDa, pI between 7.9 and 8.2, and glycosylation with glucose and/or mannose. Amino acid analysis revealed relative abundance of serine, glutamic acid and glycine, but did not show the presence of hydroxyproline. According to all these characteristics, it cannot be classified as an extensin-like protein. Another prominent wall-bound glycoprotein has a molecular mass of 66 kDa and the same pI as the 69 kDa glycoprotein. These two glycoproteins are similar also in ConA binding, rate of synthesis, and rapid incorporation into pollen tube walls. Their synthesis is strongly reduced by tunicamycin and this inhibition results in the occurrence of new polypeptides in the range of 57–61 kDa. Tunicamycin also inhibited pollen tube growth. At 10 ng ml-1 and 50 ng ml-1 the inhibitor reduced pollen tube mass after 24 h of culture by 30% and 85%, respectively. This indicates that tobacco pollen presents a system highly sensitive to tunicamycin and that cotranslational N-linked glycosylation on the rough endoplasmic reticulum is required for 66 and 69 kDa glycoprotein formation and for pollen tube growth. Although other proteins appear during pollen germination and tube growth, the new proteins occur at low levels and seem to originate through modifications of preexisting polypeptides. In contrast to 69 and 66 kDa proteins, most proteins detected by [14C]amino acid incorporation and fluorography of gels were not revealed by Coomassie blue staining.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00241888

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6

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Protein synthesis in pollen tubes: preferential formation of new species independent of transcription (1988)

Čapková, V. ; Hrabětová, E. ; Tupý, J.

Springer

Sexual plant reproduction 1 (1988), S. 150-155

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ISSN: 1432-2145

Keywords: Nicotiana ; Malus ; Pollen tube growth ; Protein synthesis ; Pollen development

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Summary Pollen tubes grown in vitro were pulselabelled for 5–30 min with [14C]amino acids, and the proteins analysed by SDS-PAGE and fluorography. In Nicotiana tabacum, the qualitative pattern of the synthesized proteins did not change significantly when the pollen was cultured for 1–24 h in different media, but total protein synthesis declined as pollen tube growth slowed down and was very low in non-growing tubes. The patterns showed preferential synthesis of proteins with apparent molecular masses of 63 and 65 kDa. The 65 kDa species, which is not synthesized during pollen maturation and is absent in ungerminated pollen grains, appears as the most intensely labelled band in both soluble and insoluble protein fractions, but accumulates as a protein non-covalently bound to pollen structures. It is also preferentially synthesized in N. sylvestris, N. alata, and in apple pollen tubes, and probably plays an important role in tube wall formation. In Nicotiana tabacum, the labelling of the 65 kDa, protein is not specifically reduced upon strong inhibition of transcription by actinomycin D, which indicates a post-transcriptional level of induction of its synthesis in germinating pollen.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00193745

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7

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The pollen-specific gene Ntp303 encodes a 69-kDa glycoprotein associated with the vegetative membranes and the cell wall (2000)

Wittink, F. R. A. ; Knuiman, B. ; Derksen, J. ; [et al.]

Springer

Sexual plant reproduction 12 (2000), S. 276-284

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ISSN: 1432-2145

Keywords: Key words NTP303 glycoprotein ; Pollen-specific Ntp303 gene ; Pollen-tube specific protein ; Plant reproduction ; Biochemical analysis ; Pollen development

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract The pollen-specific gene Ntp303 belongs to the class of late pollen specific genes. It is first transcribed directly after pollen mitosis. Biochemical properties, appearance and precise location of the NTP303 protein during pollen development and pollen tube growth were studied by amino-acid micro-sequencing, protein gel blotting and immuno-localization. Antisera were raised against recombinant proteins, encoded by sequences of the pollen-specific Ntp303 gene. The antibodies specifically recognized a 69-kDa glycoprotein. Electron-microscopic immuno-localization of the protein revealed the presence of high concentrations of the NTP303 protein at the vegetative plasma membranes that surround the vegetative cell, the generative cell and the sperm cells of pollen and pollen tubes. The generative plasma membranes of the generative cell and the sperm cells were negative. NTP303 protein was also present in the cell walls and in callose plugs. With this method it was shown that the NTP303 protein was already present in mid-bicellular pollen, after the first, asymmetrical pollen mitosis. Possible functions for the NTP303 protein are discussed in relation to its properties and its association with the vegetative plasma membranes.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/s004970050195

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