Publication Date:
2014-07-19
Description:
We produced a reference sequence of the 1-gigabase chromosome 3B of hexaploid bread wheat. By sequencing 8452 bacterial artificial chromosomes in pools, we assembled a sequence of 774 megabases carrying 5326 protein-coding genes, 1938 pseudogenes, and 85% of transposable elements. The distribution of structural and functional features along the chromosome revealed partitioning correlated with meiotic recombination. Comparative analyses indicated high wheat-specific inter- and intrachromosomal gene duplication activities that are potential sources of variability for adaption. In addition to providing a better understanding of the organization, function, and evolution of a large and polyploid genome, the availability of a high-quality sequence anchored to genetic maps will accelerate the identification of genes underlying important agronomic traits.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Choulet, Frederic -- Alberti, Adriana -- Theil, Sebastien -- Glover, Natasha -- Barbe, Valerie -- Daron, Josquin -- Pingault, Lise -- Sourdille, Pierre -- Couloux, Arnaud -- Paux, Etienne -- Leroy, Philippe -- Mangenot, Sophie -- Guilhot, Nicolas -- Le Gouis, Jacques -- Balfourier, Francois -- Alaux, Michael -- Jamilloux, Veronique -- Poulain, Julie -- Durand, Celine -- Bellec, Arnaud -- Gaspin, Christine -- Safar, Jan -- Dolezel, Jaroslav -- Rogers, Jane -- Vandepoele, Klaas -- Aury, Jean-Marc -- Mayer, Klaus -- Berges, Helene -- Quesneville, Hadi -- Wincker, Patrick -- Feuillet, Catherine -- New York, N.Y. -- Science. 2014 Jul 18;345(6194):1249721. doi: 10.1126/science.1249721.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Institut National de la Recherche Agronomique (INRA) UMR1095, Genetics, Diversity and Ecophysiology of Cereals, 5 Chemin de Beaulieu, 63039 Clermont-Ferrand, France. University Blaise Pascal, UMR1095, Genetics, Diversity and Ecophysiology of Cereals, 5 Chemin de Beaulieu, 63039 Clermont-Ferrand, France. frederic.choulet@clermont.inra.fr. ; Commissariat a l'Energie Atomique et aux Energies Alternatives, Direction des Sciences du Vivant, Institut de Genomique, Genoscope, 2 Rue Gaston Cremieux, 91000 Evry, France. ; Institut National de la Recherche Agronomique (INRA) UMR1095, Genetics, Diversity and Ecophysiology of Cereals, 5 Chemin de Beaulieu, 63039 Clermont-Ferrand, France. University Blaise Pascal, UMR1095, Genetics, Diversity and Ecophysiology of Cereals, 5 Chemin de Beaulieu, 63039 Clermont-Ferrand, France. ; INRA, UR1164 Unite de Recherche Genomique Info Research Unit in Genomics-Info, INRA de Versailles, Route de Saint-Cyr, 78026 Versailles, France. ; Centre National des Ressources Genomiques Vegetales, INRA UPR 1258, 24 Chemin de Borde Rouge, 31326 Castanet-Tolosan, France. ; Biometrie et Intelligence Artificielle, INRA, Chemin de Borde Rouge, BP 27, 31326 Castanet-Tolosan, France. ; Centre of the Region Hana for Biotechnological and Agricultural Research, Institute of Experimental Botany, Slechtitelu 31, CZ-78371 Olomouc, Czech Republic. ; The Genome Analysis Centre, Norwich, Norwich Research Park, Norwich NR4 7UH, UK. ; Department of Plant Systems Biology (VIB) and Department of Plant Biotechnology and Bioinformatics (Ghent University), Technologiepark 927, 9052 Gent, Belgium. ; Munich Information Center for Protein Sequences, Institute of Bioinformatics and Systems Biology, Helmholtz Zentrum Muenchen, D-85764 Neuherberg, Germany. ; Commissariat a l'Energie Atomique et aux Energies Alternatives, Direction des Sciences du Vivant, Institut de Genomique, Genoscope, 2 Rue Gaston Cremieux, 91000 Evry, France. CNRS UMR 8030, 2 Rue Gaston Cremieux, 91000 Evry, France. Universite d'Evry, CP5706 Evry, France.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/25035497" target="_blank"〉PubMed〈/a〉
Keywords:
Bread
;
Chromosome Segregation
;
Chromosomes, Plant/genetics/*physiology
;
DNA Transposable Elements
;
Meiosis
;
Plant Proteins/genetics
;
Polyploidy
;
Pseudogenes
;
Recombination, Genetic
;
Triticum/cytology/*genetics
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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