Publication Date:
2008-10-04
Description:
As the staple food for 35% of the world's population, wheat is one of the most important crop species. To date, sequence-based tools to accelerate wheat improvement are lacking. As part of the international effort to sequence the 17-billion-base-pair hexaploid bread wheat genome (2n = 6x = 42 chromosomes), we constructed a bacterial artificial chromosome (BAC)-based integrated physical map of the largest chromosome, 3B, that alone is 995 megabases. A chromosome-specific BAC library was used to assemble 82% of the chromosome into 1036 contigs that were anchored with 1443 molecular markers, providing a major resource for genetic and genomic studies. This physical map establishes a template for the remaining wheat chromosomes and demonstrates the feasibility of constructing physical maps in large, complex, polyploid genomes with a chromosome-based approach.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Paux, Etienne -- Sourdille, Pierre -- Salse, Jerome -- Saintenac, Cyrille -- Choulet, Frederic -- Leroy, Philippe -- Korol, Abraham -- Michalak, Monika -- Kianian, Shahryar -- Spielmeyer, Wolfgang -- Lagudah, Evans -- Somers, Daryl -- Kilian, Andrzej -- Alaux, Michael -- Vautrin, Sonia -- Berges, Helene -- Eversole, Kellye -- Appels, Rudi -- Safar, Jan -- Simkova, Hana -- Dolezel, Jaroslav -- Bernard, Michel -- Feuillet, Catherine -- New York, N.Y. -- Science. 2008 Oct 3;322(5898):101-4. doi: 10.1126/science.1161847.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Institut National de la Recherche Agronomique, Universite Blaise Pascal (INRA-UBP), UMR 1095, Genetics Diversity and Ecophysiology of Cereals, Clermont-Ferrand, France.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/18832645" target="_blank"〉PubMed〈/a〉
Keywords:
Bread
;
Chromosomes, Artificial, Bacterial
;
Chromosomes, Plant/*genetics
;
Contig Mapping
;
DNA Fingerprinting
;
Expressed Sequence Tags
;
Gene Library
;
Genome, Plant
;
Microsatellite Repeats
;
Oryza/genetics
;
*Physical Chromosome Mapping
;
Polymorphism, Genetic
;
Polyploidy
;
Radiation Hybrid Mapping
;
Triticum/*genetics
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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