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  • 1
    Electronic Resource
    Electronic Resource
    Ascorbate Increases the 1,25 Dihydroxyvitamin D3-Induced Monocytic Differentiation of HL-60 Cells (1996)
    Springer
    Calcified tissue international 59 (1996), S. 277-282 
    Details
    ISSN: 1432-0827
    Keywords: Key words: Ascorbate — Calcitriol — 1,25 Dihydroxyvitamin D3— Leukemia — HL-60 — Cell Differentiation.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Abstract. 1,25 Dihydroxyvitamin D3 (calcitriol) induces differentiation of HL-60 leukemia cells. We studied the in vitro effect of a physiological concentration of ascorbate as potentiator of 1,25 dihydroxyvitamin D3 [(OH)2D3] activity by determining different markers of differentiation: nitroblue tetrazolium reduction, nonspecific esterase activity, and the expression of CD11b and CD14 surface antigens. Nitroblue tetrazolium reduction and nonspecific esterase activity increased up to 50% in the presence of both 1,25 (OH)2D3 plus 0.2 mM ascorbate (ASC), compared with (OH)2D3 as a unique agent. ASC also increased the expression of specific surface antigens (CD11b and CD14) during differentiation induced by 1,25 (OH)2D3, the effect being more pronounced after 48 hours of treatment with 10−8 M 1,25 (OH)2D3. Furthermore, 1,25 (OH)2D3 alone increased intracellular cAMP level during differentiation, and the addition of ASC increased its concentration from 60 to 100% above the level reached with 1,25 (OH)2D3 as unique agent. ASC did not enhance the antiproliferative effect of calcitriol, suggesting that it only affects the ability of 1,25 (OH)2D3 to promote differentiation of HL-60 cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002239900123
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  • 2
    Electronic Resource
    Electronic Resource
    NADH-ascorbate free radical and -ferricyanide reductase activities represent different levels of plasma membrane electron transport (1993)
    Springer
    Journal of bioenergetics and biomembranes 25 (1993), S. 411-417 
    Details
    ISSN: 1573-6881
    Keywords: Plasma membrane ; liver ; ascorbate ; dehydrogenases ; clathrin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Physics
    Notes: Abstract Plasma membranes isolated from rat liver by two-phase partition exhibited dehydrogenase activities for ascorbate free radical (AFR) and ferricyanide reduction in a ratio of specific activities of 1 : 40. NADH-AFR reductase could not be solubilized by detergents from plasma membrane fractions. NADH-AFR reductase was inhibited in both clathrin-depleted membrane and membranes incubated with anti-clathrin antiserum. This activity was reconstituted in plasma membranes in proportion to the amount of clathrin-enriched supernatant added. NADH ferricyanide reductase was unaffected by both clathrin-depletion and antibody incubation and was fully solubilized by detergents. Also, wheat germ agglutinin only inhibited NADH-AFR reductase. The findings suggest that NADH-AFR reductase and NADH-ferricyanide reductase activities of plasma membrane represent different levels of the electron transport chain. The inability of the NADH-AFR reductase to survive detergent solubilization might indicate the involvement of more than one protein in the electron transport from NADH to the AFR but not to ferricyanide.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00762467
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  • 3
    Electronic Resource
    Electronic Resource
    Plasma membrane redox system during HL-60 induced differentiation (1995)
    Springer
    Protoplasma 184 (1995), S. 163-167 
    Details
    ISSN: 1615-6102
    Keywords: Redox system ; Differentiation ; HL-60 cell ; Calcitriol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary HL-60 cells were induced to differentiate by exposure to TPA or 1,25-dihydroxyvitamin D3 (calcitriol). Induction with TPA was in parallel with a modulation of transmembrane redox system. After addition of 2 ng/m1 TPA, transient increases in ferricyanide reductase activity, NAD(H) intracellular levels and short-term response of NAD(H) to 0.4 mM ferricyanide were observed. The role of ascorbate on the differentiation induced by calcitriol also was studied. When HL-60 cells were exposed to 10−8 M calcitriol in the presence of 0.2 mM ascorbate, specific differentiation markers as NBT reduction or surface antigen CD11b increased significantly with respect to values obtained from treatments with calcitriol alone.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01276915
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  • 4
    Electronic Resource
    Electronic Resource
    Xanthine accumulation and vacuolization inChlamydomonas reinhardtii cells (1995)
    Springer
    Protoplasma 186 (1995), S. 93-98 
    Details
    ISSN: 1615-6102
    Keywords: Chlamydomonas ; Accumulation ; Purines ; Vacuole ; Xanthine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Utilization of xanthine as the sole nitrogen source for growth byChlamydomonas reinhardtii cells involved the formation of a transient, intracellular pool of xanthine. Up to 20% of the total xanthine supplied to the medium was not assimilated after uptake but stored in the cells at concentrations that exceeded xanthine solubility in water. At the subcellular level, a massive accumulation of starch grains in the chloroplast and the appearance of many vacuoles in the cytoplasm distinguished xanthine-grown from ammonium-grown cells. Starch accumulation, but not development of vacuoles, was also observed in N-starved cells. Uptake experiments with radio-labelled xanthine showed that this accumulates only in the cytoplasm, most probably inside vacuoles. The electron-dense material observed in vacuoles of xanthine-grown cells suggests that the intracellular xanthine is in part solid xanthine.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01276941
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  • 5
    Electronic Resource
    Electronic Resource
    Plasma membrane glycoproteins during anuran amphibian epidermal development (1993)
    Springer
    Protoplasma 172 (1993), S. 136-144 
    Details
    ISSN: 1615-6102
    Keywords: Amphibian epidermis ; Metamorphosis ; Osmoregulation ; Plasma membrane ; Glycoprotein ; Lectin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A cytochemical and biochemical study of galactose (Gal) and N-acetyl-glucosamine (GlcNAc) containing glycoproteins of the anuran amphibian epidermis during development has been carried out. In premetamorphic tadpoles, theGriffonia simplicifolia II lectin (GS II, specific for N-acetyl glucosamine) bound to a glycoprotein of 49 kDa in the plasma membrane of all the epidermal strata showing a basal-to-apical binding gradient. During metamorphic climax GS II labeling was progressively polarized to the outermost plasma membrane. In epidermis from juveniles and adults the staining was observed mainly in a 52 kDa band.Griffonia simplicifolia I lectin (GS I, specific for galactose) also bound to a glycoprotein of about 49 kDa in tadpoles and 52 kDa in frogs. Furthermore, a GS I labeling in bands of about 110–150 kDa appears during metamorphosis. After this process, a definitive pattern of lectin staining and K+-stimulated, ouabain-sensitive p-nitrophenyl phosphatase activity is established.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01379371
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