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  • 1
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    Iron reverses impermeable chelator inhibition of DNA synthesis in CCl 39 cells. (1994)
    National Academy of Sciences
    Details
    Publication Date: 1994-08-16
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
    Published by National Academy of Sciences
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    PAPER CURRENT
  • 2
    Electronic Resource
    Electronic Resource
    Ascorbate Increases the 1,25 Dihydroxyvitamin D3-Induced Monocytic Differentiation of HL-60 Cells (1996)
    Springer
    Calcified tissue international 59 (1996), S. 277-282 
    Details
    ISSN: 1432-0827
    Keywords: Key words: Ascorbate — Calcitriol — 1,25 Dihydroxyvitamin D3— Leukemia — HL-60 — Cell Differentiation.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Abstract. 1,25 Dihydroxyvitamin D3 (calcitriol) induces differentiation of HL-60 leukemia cells. We studied the in vitro effect of a physiological concentration of ascorbate as potentiator of 1,25 dihydroxyvitamin D3 [(OH)2D3] activity by determining different markers of differentiation: nitroblue tetrazolium reduction, nonspecific esterase activity, and the expression of CD11b and CD14 surface antigens. Nitroblue tetrazolium reduction and nonspecific esterase activity increased up to 50% in the presence of both 1,25 (OH)2D3 plus 0.2 mM ascorbate (ASC), compared with (OH)2D3 as a unique agent. ASC also increased the expression of specific surface antigens (CD11b and CD14) during differentiation induced by 1,25 (OH)2D3, the effect being more pronounced after 48 hours of treatment with 10−8 M 1,25 (OH)2D3. Furthermore, 1,25 (OH)2D3 alone increased intracellular cAMP level during differentiation, and the addition of ASC increased its concentration from 60 to 100% above the level reached with 1,25 (OH)2D3 as unique agent. ASC did not enhance the antiproliferative effect of calcitriol, suggesting that it only affects the ability of 1,25 (OH)2D3 to promote differentiation of HL-60 cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002239900123
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Ascorbate potentiates serum-induced S phase entry of quiescent 3T3 cells (1992)
    Springer
    Protoplasma 169 (1992), S. 85-87 
    Details
    ISSN: 1615-6102
    Keywords: Ascorbate ; S phase ; BALB/c 3T3 cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Arrested BALB/c 3T3 cells were induced to the G0-G1 transition by fetal calf serum (FCS) and S phase entry was measured by [3H]thymidine incorporation as an index of DNA synthesis. [3H]Thymidine uptake was proportional to FCS concentration. Ascorbate (ASC) itself was unable to increase DNA synthesis in these cells but potentiated it in the presence of both 1% and 10% FCS. [3H]Thymidine uptake profile was similar with and without ASC, and showing at 24 h an ASC stimulation of 69% in the presence of 1% FCS and 58% with 10% FCS. These data are discussed in reference to the participation of ASC on plasma membrane energization for membrane translocations and transport.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01343373
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  • 4
    Electronic Resource
    Electronic Resource
    Vitamin C stabilization as a consequence of the plasma membrane redox system (1995)
    Springer
    Protoplasma 184 (1995), S. 229-232 
    Details
    ISSN: 1615-6102
    Keywords: Ascorbate stabilization ; Ascorbate free radical ; Plasma membrane redox system ; HL-60 cells ; Cyclic AMP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Ascorbate is stabilized in the presence of HL-60 cells. Our results showed that cAMP derivatives and agents that increase cAMP stimulate the ability of HL-60 cells to stabilize ascorbate. On the other hand, tunicamycin, a glycosilation-interfering agent, inhibited this ability. The ascorbate stabilization in the presence of HL-60 cells has been questioned as a simple chemical effect. Further properties and controls about the enzymatic nature of this stabilization are described and discussed. This data, together with hormonal regulation, support the hypothesis that an enzymatic redox system located at the plasma membrane is responsible of the extracellular ascorbate stabilization by HL-60 cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01276925
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  • 5
    Electronic Resource
    Electronic Resource
    Iron at the cell surface controls both DNA synthesis and plasma membrane redox system (1995)
    Springer
    Protoplasma 184 (1995), S. 233-237 
    Details
    ISSN: 1615-6102
    Keywords: Iron ; Bathophenanthroline disulfonate ; Plasma membrane electron transport ; DNA synthesis ; Growth factors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Addition of the impermeable iron II chelator bathophenanthroline disulfonate (BPS) to cultured Chinese hamster lung fibroblast (CCL 39 cells) inhibits DNA synthesis but not protein synthesis or cytoplasmic alkalinization, when cell growth is initiated with growth factors such as EGF plus insulin, thrombin, or ceruloplasmin. The BPS inhibition is reversed by addition of stoichiometric ferrous iron at stoichiometric concentration. BPS does not inhibit cell growth stimulated by fetal calf serum. The effect of the BPS differs from the inhibition of growth by hydroxyurea which acts on the ribonucleotide reductase. The BPS treatment leads to release of iron from the cells as determined by BPS iron II complex formation over 90 min. Cells treated with BPS just during starvation period cannot re-initiate DNA synthesis after mitogen stimulation even if BPS is removed from the medium and cells are previously washed. BPS treatment also inhibits transplasma membrane electron which is restored by incubation of cells with 10 μM ferric ammonium citrate. Growth factor stimulation of DNA synthesis is restored by addition of 1 μM ferrous ammonium sulfate or ferric ammonium citrate, or 0.1 μM diferric transferrin. Copper, cobalt, nickel, zinc, gallium, aluminum, or apotransferrin cannot restore the activity. The BPS effect is consistent with removal of iron from a site on the cell surface which controls electron transport and DNA synthesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01276926
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  • 6
    Electronic Resource
    Electronic Resource
    Plasma membrane redox system during HL-60 induced differentiation (1995)
    Springer
    Protoplasma 184 (1995), S. 163-167 
    Details
    ISSN: 1615-6102
    Keywords: Redox system ; Differentiation ; HL-60 cell ; Calcitriol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary HL-60 cells were induced to differentiate by exposure to TPA or 1,25-dihydroxyvitamin D3 (calcitriol). Induction with TPA was in parallel with a modulation of transmembrane redox system. After addition of 2 ng/m1 TPA, transient increases in ferricyanide reductase activity, NAD(H) intracellular levels and short-term response of NAD(H) to 0.4 mM ferricyanide were observed. The role of ascorbate on the differentiation induced by calcitriol also was studied. When HL-60 cells were exposed to 10−8 M calcitriol in the presence of 0.2 mM ascorbate, specific differentiation markers as NBT reduction or surface antigen CD11b increased significantly with respect to values obtained from treatments with calcitriol alone.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01276915
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  • 7
    Electronic Resource
    Electronic Resource
    Stimulation of onion root elongation by ascorbate and ascorbate free radical inAllium cepa L. (1995)
    Springer
    Protoplasma 184 (1995), S. 31-35 
    Details
    ISSN: 1615-6102
    Keywords: Ascorbate oxidation ; Cell elongation ; Onion root ; Plasma membrane redox system
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We report that ascorbate free radical stimulates onion root growth at 15 °C and 25 °C. The fully reduced form, ascorbate, also stimulates root elongation if culture conditions allow its oxidation. When ascorbate oxidation was inhibited, no stimulation of root growth was found. The effect of the fully oxidized form, dehydroascorbate, was inhibitory. We show also that ascorbate free radical generated by ascorbate oxidation, is reduced back probably by a transplasmalemma reductase. These results are discussed on the basis of an activation of a transplasma membrane redox system likely involved in processes related to cell growth.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01276898
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