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  • 1
    Electronic Resource
    Electronic Resource
    In-vitro formation of a new fibrous attachment to human dental roots in the presence of autologous serum (1989)
    Springer
    Cell & tissue research 258 (1989), S. 125-135 
    Details
    ISSN: 1432-0878
    Keywords: Periodontal ligament cells ; Alveolar bone cells ; Autologous serum ; Cementum ; Dentin ; Cell culture ; Human
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Recent studies have accomplished the establishment of a collagenous fiber-fringe matrix upon dental root surfaces in vitro. The present study was undertaken to follow the development of such a matrix in vitro and to test the possible effects of root surface treatments upon this matrix. Periodontal ligament cells, 0.1 to 0.2-mm thick dental root discs, and alveolar bone cells were derived after extraction from four partially erupted third molars and the accompanying interradicular bony septa of 1 male patient. Autologous serum was obtained by venipuncture. Cultures were initiated by delivering a 1-ml suspension of 50000 tritiated thymidine-labeled periodontal ligament cells and 50000 alveolar bone cells onto each of 42 culture sets. The following day, demineralized or non-demineralized root discs treated with autologous serum, fibronectin or complete medium were placed in pairs, separated by a 0.1–1.0 mm gap, upon the initial cell layer. Representative cultures were terminated after 2, 3, 4, 5 and 6 weeks, and processed for light- and electron microscopy, morphometric analysis and autoradiography. An outstanding feature of the early cultures (2, 3 and 4 weeks) was a patchwise, random distribution of matrix making a precise developmental study impossible, although collagen fibrils were produced within the first 2 weeks. Some 3-week cultures already demonstrated a mature fiber-fringe characterized electron-microscopically as oriented, densely packed collagen fibrils closely abutting the cementum-lined root discs. The treatments (including autologous serum) used in this study had no appreciable morphologic or morphometric effect upon the fiber-fringe formed. Because none of the cultures in the present or past studies have demonstrated a true cementoid matrix, this model may not be suitable for the in-vitro study of cementum formation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223152
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  • 2
    Electronic Resource
    Electronic Resource
    Formation of a new fibrous attachment to human dental roots (1988)
    Springer
    Cell & tissue research 254 (1988), S. 659-670 
    Details
    ISSN: 1432-0878
    Keywords: Periodontal ligament cells ; Cementum ; Cell culture ; Regeneration ; Human
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary This study was performed to improve currently employed in vitro models for the study of periodontal regeneration by using a porous filter upon which periodontal ligament cells were grown. Periodontal ligament cells were harvested and 0.3 mm root discs cut from three partially erupted and extracted third molar teeth of one patient. Experimental culturing was performed by seeding periodontal ligament cell suspensions on Puropor-200 filters supported by wire-mesh grids in Grobstein Petri dishes. The following day, an interdental space of 0.1 to 0.3 mm was created by gently placing two dental root discs upon the filter. Cultures were terminated after 42, 56, 112 and 124 days, and processed for light- and electron microscopy. Collagen fibril diameters were measured. Adjacent and often attached to large areas of cementum-lined root discs, a dense fiber fringe developed. This fiber fringe was not found on dentinlined root discs. Although less organized, older cultures demonstrated a similar disc-culture interface, which depended upon the presence or absence of original root cementum. Collagen fibrils of early cultures had a mean diameter of about 42 nm, while in older cultures the diameters ranged from 47 to 68 nm. It is concluded that the fibrous matrix attached to cementum-lined root discs somewhat resembles the initial stages of the formation of dental root cementum in vivo.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226517
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  • 3
    Electronic Resource
    Electronic Resource
    In-vitro formation of a collagenous matrix upon previously diseased and experimentally treated cemental root surfaces (1990)
    Springer
    Cell & tissue research 259 (1990), S. 603-606 
    Details
    ISSN: 1432-0878
    Keywords: Periodontal ligament cells ; Alveolar bone cells ; Cementum ; Cell culture ; Human
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A diseased and mechanically treated surface of root cementum is known, clinically, to favor periodontal regeneration. The present investigation was undertaken to test whether previously diseased and experimentally treated root surfaces can support the in-vitro formation of a new collagenous matrix. Three teeth extracted for advanced periodontitis were treated first with 5% sodium hypochlorite for 2 h to remove all organic material from the root surface. After the healthy, apical one third of the root was cut off, the roots were scaled with moderate pressure to remove visible calculus. Non-demineralized root discs were cut and placed on a co-culture of periodontal ligament- and alveolar bone-derived cells. After 7 weeks in culture, either one of two matrix types was found along the root surface. The most frequent matrix consisted of clusters of cells layered within densely aggregated collagen fibrils. The other, less frequent matrix consisted of loosely arranged collagen fibrils adjacent to the cemental surface. The findings support the notion that, in vitro, a collagenous matrix is formed in contact to diseased and experimentally treated root surfaces. However, the smooth, non-demineralized and scaled cemental surface does not appear to be a suitable substrate for interdigitation with newly produced collagen fibrils.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01740790
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  • 4
    Electronic Resource
    Electronic Resource
    Formation of a new fibrous attachment to human dental roots (1989)
    Springer
    Cell & tissue research 255 (1989), S. 631-639 
    Details
    ISSN: 1432-0878
    Keywords: Periodontal ligament cells ; Alveolar bone cells ; Cementum ; Cell culture ; Regeneration ; Human
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Human periodontal ligament cells have been shown to produce a new fibrous attachment at the surface of scaled dental root discs in vitro. The purpose of this investigation was to answer the question whether cells derived from human alveolar bone would enhance this attachment. Three partially erupted third molars were extracted and collected from one female patient and used for harvesting periodontal ligament cells as well as for cutting 0.3 mm dental root discs. Cells derived from alveolar bone were obtained by enzymatic digestion of bone chips harvested after extraction of wisdom teeth from three additional patients. Experimental cultures were prepared by seeding 1.0 × 106 suspensions of periodontal ligament cells onto five Puropor-200 filters. The following day, root disc pairs were placed on the cell layer, leaving a gap (interdental space) of 0.1–0.3 mm. Five days later, all cultures received equal aliquots of alveolar bone cells. The cultures were terminated and processed for microscopic and morphometric evaluation after 56, 112 and 124 days. All cultures demonstrated a dense fiber-fringe attachment along most of the cementum-lined surfaces of root discs. Adjacent to other root-disc surfaces, cells were surrounded by halos of their collagenous product. Mean diameters of collagen fibrils for the 56-, 112- and the two 124-day cultures were 64.6, 48.9 and 62.6 nm, respectively. Compared to results obtained in this system with periodontal ligament cells alone (Bernstein et al. 1988), the fiber fringe in this experiment was denser, composed of collagen fibrils with a larger diameter, and maintained for longer duration in culture. These findings support the hypothesis that cells from the alveolar bone participate, as a normal component of the periodontal ligament, in the maintenance and repair of periodontal ligament attachment.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00218801
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  • 5
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    In-vitro formation of a collagenous matrix upon previously diseased and experimentally treated cemental root surfaces (1990)
    Springer
    Details
    Publication Date: 1990-10-01
    Print ISSN: 0302-766X
    Electronic ISSN: 1432-0878
    Topics: Biology , Medicine
    Published by Springer
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