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  • 1
    Electronic Resource
    Electronic Resource
    Cytochromes of Rhizobium (1958)
    [s.l.] : Nature Publishing Group
    Nature 182 (1958), S. 1174-1174 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] While observing with a microspectroscope the reduction of legh moglobin in fresh homogenates of effective soybean or clover nodules made anaerobic by endogenous respiration, we saw also a sharp absorption band appearing near 551 mµ. This was later identified as the a-absorption band of ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/1821174a0
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  • 2
    Electronic Resource
    Electronic Resource
    Oxygenation of Leghæmoglobin in Soybean Root-Nodules in Relation to the External Oxygen Tension (1962)
    [s.l.] : Nature Publishing Group
    Nature 194 (1962), S. 1059-1061 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] TEGHBMOGLOBIN, the characteristic red pig-I J ment of legume root nodules, seems to be involved in some component reaction of the fixation of atmospheric nitrogen which these organs accomplish1. The role of this hsemoprotein has variously been postulated to be an oxidation-reduction catalyst, an ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/1941059a0
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  • 3
    Electronic Resource
    Electronic Resource
    Biochemistry of Symbiotic Nitrogen Fixation in Legumes (1971)
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Plant Physiology 22 (1971), S. 121-140 
    Details
    ISSN: 0066-4294
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1146/annurev.pp.22.060171.001005
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  • 4
    Electronic Resource
    Electronic Resource
    Microaerobic respiration and oxidative phosphorylation by soybean nodule mitochondria: implications for nitrogen fixation (1995)
    Oxford, UK : Blackwell Publishing Ltd
    Plant, cell & environment 18 (1995), S. 0 
    Details
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The infected cells of soybean (Glycine max) root nodules require ATP production for ammonia assimilation and purine synthesis under microaerobic conditions. It is likely that the bulk of this demand is supplied through mitochondrial oxidative phosphorylation. Mitochondria purified from root nodules respired and synthesized ATP in sub-micromolar oxygen concentrations as measured by leghaemoglobin spectroscopy and luciferase luminescence. Both oxygen uptake and the apparent ATP/O ratio declined significantly as the oxygen concentration fell below 100 μmol m−3. Cytochrome-pathway respiration by root nodule mitochondria had a higher apparent affinity for oxygen (Km 50 μmol m−3) than did mitochondria isolated from roots (Km 125 μmol m−3). Electron micrographs showed that mitochondria predominated at the periphery of infected cells adjacent to gas-filled intercellular spaces, where the oxygen concentration is predicted to be highest. Calculations of oxygen concentration and nitrogen fixation rates on an infected cell basis suggest that the measured rates of ATP production by isolated mitochondria are sufficient for the quantifiable in vivo requirements of ammonia assimilation and purine synthesis. The possible roles of mitochondrial respiration in the control of infected cell metabolism are also discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3040.1995.tb00574.x
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  • 5
    Electronic Resource
    Electronic Resource
    LEGHEMOGLOBIN: THE ROLE OF HEMOGLOBIN IN THE NITROGEN-FIXING LEGUME ROOT NODULE (1975)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 244 (1975), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1975.tb41519.x
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  • 6
    Electronic Resource
    Electronic Resource
    Leghaemoglobin within bacteroid-enclosing membrane envelopes from soybean root nodules (1981)
    Springer
    Planta 152 (1981), S. 534-543 
    Details
    ISSN: 1432-2048
    Keywords: Bacteroids ; Glycine ; Leghaemoglobin ; Membrane envelopes (root nodules) ; Root nodules
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Methods are reported for the preparation from soybean (Glycine max (L.) Merr.) root nodules, of well-washed, intact membrane envelopes containing bacteroids. The intact envelopes are of much lower density than the bacteroids within and therefore only low speed centrifugation (approx. 150 g) may be used. The optimum osmotic strength is 600 mOsm/kg H2O. The envelope contents were recovered following mild osmotic shock and-or hard centrifugal packing at 〉10,000 g. Extracts prepared in this way contained leghaemoglobin (identified spectrophotometrically), low-molecular-weight fluorescent materials and other components which are yet to be identified. Envelope leghaemoglobin did not react with specific antibody until the envelopes were ruptured. 131I-Labelled leghaemoglobin or bovine serum albumin, added during initial breakage of nodule cells, was not released when envelopes were ruptured to release leghaemoglobin. It is therefore concluded that this leghaemoglobin is located within the envelope space and did not arise from adhering or occluded cytosol leghaemoglobin. Based on the number and dimensions of microscopically intact envelopes in these preparations, the concentration within that space was in the range 178–523 μM. Based on these estimates, leghaemoglobin within envelopes represented about one third of the total amount present in the nodule cells. Flat-bed isoelectric focusing of partially-purified envelope leghaemoglobin demonstrated that the latter contained all of the leghaemoglobin components previously reported for soybean nodules and an additional minor component focusing between leghaemoglobins a and b.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00380824
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  • 7
    Electronic Resource
    Electronic Resource
    Some Australian studies relating to the long-term effects of the inoculation of legume seeds (1970)
    Springer
    Plant and soil 32 (1970), S. 727-736 
    Details
    ISSN: 1573-5036
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Conclusions The work outlined in this paper is only a small part of the legume inoculation work being undertaken in Australia and it deals only with temperate pasture problems. It has been presented as an example of certain principles related to the production of legumes, which have very general applications. These may be summarized as follows: 1. Successful inoculation is frequently an essential part of obtaining maximum seedling establishment. 2. Inoculated strains ofRhizobium may not persist and responses may be transitory. 3. If the natural population which develops is inferior in terms of nitrogen fixation, it will be necessary to further select for persistent strains of high nitrogen fixing capacity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01372902
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  • 8
    Electronic Resource
    Electronic Resource
    Measurement of N2 fixation in maize (Zea mays L.)—ricebean (Vigna umbellata [Thunb.] Ohwi and Ohashi) intercrops (1988)
    Springer
    Plant and soil 108 (1988), S. 125-135 
    Details
    ISSN: 1573-5036
    Keywords: intercropping ; N2 fixation ; natural15N abundance ; ureides ; Vigna umbellata ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The yield of N in maize (Zea mays L.) and ricebean (Vigna umbellata [Thumb.] Ohwi and Ohashi) were compared on a Tropoqualf soil in North Thailand in 1984 and 1985. Both species were grown in field plots in monoculture or as intercrops at a constant planting density equivalent to 8 maize or 16 ricebean plants per m2. The contribution of symbiotic N2 fixation to ricebean growth was estimated from measurements of the natural abundance of15N (δ15N) in shoot nitrogen and from analysis of ureides in xylem sap vacuumextracted from detached stems. The natural abundance of15N in the intercropped ricebean was found to be considerably less than that in monoculture in both growing seasons. Using maize and a weed (Ageratum conyzoides L.) as non-fixing15N reference plants the proportions (P 15N) of ricebean shoot N derived from N2 fixation ranged from 0.27 to 0.36 in monoculture ricebean up to 0.86 when grown in a 75% maize: 25% ricebean intercrop. When glasshouse-derived calibration curves were used to calculate plant proportional N2 fixation (Pur) from the relative ureide contents of field collected xylem exudates, the contribution of N2 fixation to ricebean N yields throughout the 1985 growing season were greater in intercrop than in monocrop even at the lowest maize:legume ratio (25∶75). Seasonal patterns of sap ureide abundance indicated that N2 fixation was greatest at the time of ricebean podset. The averagePur andP 15N in ricebean during the first 90 days of growth showed identical rankings of monocrop and intercrop treatments in terms of N2 fixation, although the two sets ofP values were different. Nonetheless, seasonal estimates of N2 fixation during the entire 147 days of legume growth determined from ureide analyses indicated that equivalent amounts of N could be fixed by ricebean in a 75∶25 intercrop and in monoculture despite the former being planted at one-quarter the density.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02370107
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  • 9
    Electronic Resource
    Electronic Resource
    Adaptation of methods for evaluating N2 fixation in food legumes and legume cover crops (1988)
    Springer
    Plant and soil 108 (1988), S. 143-150 
    Details
    ISSN: 1573-5036
    Keywords: legumes ; N2 fixation ; 15N analysis ; ureides ; xylem exudate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Methods for partitioning the nitrogen assimilated by nodulated legumes, between nitrogen derived from soil sources and from N2 fixation, are described as applied in peninsular Malaysia. The analysis of nitrogenous components translocated from the roots to the shoots of nodulated plants in the xylem sap is outlined, with some precautions to be observed for applications in the tropics. Some examples of the use of the technique in surverying apparent N2 fixation by tropical legumes, in studying interrow cropping in plantation systems and in assessing effects of experimental treatments on N2 fixation by food legumes, are described. Techniques for assesing N2 fixation by means of15N abundance have been used to show that applications of nitrogenous fertilizers commonly used in Malaysia for soybeans depress N2 fixation, that similar results are obtained with natural abundance and15N-enrichment methods and that, in at least two locations in Malaysia, differences between the natural abundance of15N in plant-available soil nitrogen and in atmospheric N2 are great enough to permit application to measurement of N2 fixation by leguminous crops.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02370109
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  • 10
    Electronic Resource
    Electronic Resource
    Some Australian studies relating to the long-term effects of the inoculation of legume seeds (1970)
    Springer
    Plant and soil 32 (1970), S. 727-736 
    Details
    ISSN: 1573-5036
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Conclusions The work outlined in this paper is only a small part of the legume inoculation work being undertaken in Australia and it deals only with temperate pasture problems. It has been presented as an example of certain principles related to the production of legumes, which have very general applications. These may be summarized as follows: 1. Successful inoculation is frequently an essential part of obtaining maximum seedling establishment. 2. Inoculated strains ofRhizobium may not persist and responses may be transitory. 3. If the natural population which develops is inferior in terms of nitrogen fixation, it will be necessary to further select for persistent strains of high nitrogen fixing capacity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01372902
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