ISSN:
1040-0397
Keywords:
Elearochemical immunoassay
;
Alkaline phosphatase
;
Kinetic deteaion
;
Cortisol
;
Thyroxine binding globulin
;
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Kinetic measurement of an alkaline phosphatase enzyme label was performed with amperometric detection using conversion of substrate 4-aminophenyl phosphate to the electroactive phenolic, measured at +125 to +200 mV tvs. Ag/AgCl. Calibration plots for thyroxine-binding globulin (TBG) and cortisol were obtained by excess reagent and limited reagent immunoassays, respectively, which were comparable with those obtained using colorimetric detection. These provided a measurement range of 31-1000 μg/L for TBG and 100-2000 nM for cortisol. An antibody-bound label was monitored in this system, and covering the electrode with a simple dialysis or polycarbonate membrane avoided electrode fouling. The kinetic mode of detection reduced assay time and helped to simplify the assay protocol.
Additional Material:
5 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elan.1140060705
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