Electronic Resource
Springer
Molecular genetics and genomics
190 (1983), S. 295-299
ISSN:
1617-4623
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary An assay for the detection of pyrimidine dimers has been adapted for use in permeabilized Neurospora crassa cells. Dimers are produced as a linear function of UV dose. Each Jm-2 of UV fluence yields 0.40 dimers per 108 daltons of DNA. Wild type Neurospora removes dimers during post-irradiation incubation. This excision repair process proceeds much more rapidly in nutritive medium (80% repair in 60 min) than in phosphate buffer (25% repair in 60 min). Cycloheximide added immediately after a damaging UV dose inhibits repair in growth medium but does not affect repair in phosphate buffer. A low dose of UV, 2 Jm-2, presented prior to the damaging dose, 22 Jm-2, increases excision repair activity in phosphate buffer. The data suggests that an inducible as well as a constitutive excision repair process is present in this eukaryotic organism.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00330654
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