Publication Date:
2020-09-28
Description:
To visualize the fine structure of compacted DNA in Synechococcus elongatus PCC 7942, which appears at a specific time in the regular light/dark cycle prior to cell division, Chrom EM (Ou et al., 2017) with some modifications was applied. After staining DNA with DRAQ5, the cells were fixed and irradiated by red laser in the presence of DAB, and subsequently fixed with OsO4. A system with He-Ne laser (633 nm) was set up for efficient irradiation of the bacterial cells in aqueous solution. The compacted DNA was visualized by TEM, in ultrathin sections as electron dense staining by osmium black.
Print ISSN:
2050-5698
Electronic ISSN:
2050-5701
Topics:
Electrical Engineering, Measurement and Control Technology
,
Natural Sciences in General
,
Physics
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