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  • 1
    ISSN: 1572-9702
    Keywords: Amblyomma variegatum ; pheromone ; tick ; geographic range ; heartwater
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The tropical bont tick, Amblyomma variegatum, transmits heartwater in sub-Saharan Africa and in the Caribbean. This species has a broad geographic distribution, ranging from Madagascar and other islands in the Indian Ocean through most of sub-Saharan Africa, to several islands in the eastern Caribbean Sea. Blood fed male A. variegatum secrete an attraction–aggregation–attachment (AAA) pheromone which, combined with CO2, excites host finding and formation of feeding clusters of these ticks. However, it is not known whether the composition of the pheromone varies throughout A. variegatum’s geographic range. Extracts of fed male ticks were examined for phenols and volatile organic acids by gas chromatography and mass spectrometry to determine whether differences occur in the pheromone components of populations of this species across the geographic range (Guadeloupe, Zimbabwe, Zambia and Rwanda). No significant difference in the chemical composition of the pheromone in relation to geographic range was found. No significant differences in rates of attachment in response to native versus foreign extracts were found in on-host attachment tests comparing ticks from two countries, Guadeloupe (Caribbean) and Zimbabwe (African). This finding was confirmed in more detailed studies with ticks from Guadeloupe and four African countries (Kenya, Rwanda, Zambia and Zimbabwe). On-host attachment assays from these countries did not detect consistent differences in response to extracts from different locations. In an olfactometer bioassay, females were not consistently more attracted to extracts from their native locality than from any of the foreign localities. We conclude that despite the widespread distribution of A. variegatum over both hemispheres, no significant differences in pheromone composition or biological responses to male tick pheromone secretions occur.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Experimental and applied acarology 11 (1991), S. 9-21 
    ISSN: 1572-9702
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Amblyomma americanum andA. maculatum were studied to determine if genital sex pheromones were present, as reported inDermacentor species. Chemical analysis of methanol extracts of the anterior reproductive tracts (source of the genital sex pheromone) ofA. americanum andA. maculatum confirmed the presence of the same fatty acids that act as components of the genital sex pheromone inDermacentor variabilis andD. andersoni. In bothAmblyomma species, removal of the anterior reproductive-tract of fed females eliminated the male copulatory response. ForA. americanum, use of anterior reproductive tract extracts made with water, methanol, ether and hexane elicited significant probing and copulatory responses by conspecific males. However, inA. maculatum, use of anterior reproductive-tract extracts made with water, methanol, ether, chloroform: methanol (4∶1), hexane and pentane did not restore mating response by males. Treatment with known components ofDermacentor genital sex pheromones elicited weak but significant responses inA. americanum, but did not restore male mating responses inA. maculatum. These results indicate that genital sex pheromones exist inA. americanum and are not restricted to the genusDermacentor. Characteristics of theAmblyomma genital sex pheromone, however, do differ considerably from those inDermacentor. No genital sex pheromone was present inA. maculatum.
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  • 3
    ISSN: 1572-9702
    Keywords: Amblyomma hebraeum ; bont ticks ; acaricides ; tick control pheromones ; tail-tag decoy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A large-scale field test using pheromone-acaricide-impregnated plastic tail-tag decoys demonstrated excellent efficacy of these devices for control of the bont tick,Amblyomma hebraeum, on cattle in Zimbabwe. The tail tags were impregnated with a mixture containingo-nitrophenol, methyl salicylate, 2,6-dichlorophenol and phenylacetaldehyde and one of three different acaricides (cyfluthrin, flumethrin or alphacypermethrin).o-Nitrophenol and methyl salicylate are components of theA. hebraeum attraction-aggregation-attachment pheromone, while 2,6-dichlorophenol and phenylacetaldehyde are proven attractants for this tick. Botho-nitrophenol and methyl salicylate were lost gradually from the tags over 12 and 14 week periods, respectively. In field trials, tick counts were compared between cattle that received tail tags either impregnated with pheromone mixture alone, cyfluthrin and pheromone mixture, flumethrin and pheromone mixture, alphacypermethrin and pheromone mixture or were left untreated. During the first 3 month trial period, control of adult bont ticks was 94.9% with cyfluthrin tail tags and 87.5% with flumethrin tail tags. In general, there was no significant difference in bont tick numbers on cattle without tags and those with tail tags containing pheromone only. When the trial was repeated for another 3 month period, control of bont ticks with tail tags containing cyfluthrin and flumethrin was 99.3 and 95.1%, respectively. However, control of bont ticks using alphacypermethrin was only 79.2%. Overall, retention of tail tags was excellent although some loss was encountered during the rainy season. In addition to controlling bont ticks, the tail tags provided moderate control of other tick species (Rhipicephalus evertsi evertsi, Rhipicephalus zambeziensis andHyalomma spp.) simultaneously infesting cattle in the trials.
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  • 4
    ISSN: 1573-1561
    Keywords: Insecta ; Diptera ; Culicidae ; Aedes albopictus ; Aedes aegypti ; oviposition ; attractants ; behavior
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Oviposition responses of gravidAedes albopictus andAe. aegypti were evaluated to natural organic infusions (hay infusion, larval rearing water, and field-collected larval water) as well as compounds isolated from hay infusion (3-methylindole, 4-methylphenol, 4-ethylphenol, indole, and phenol) known to elicit oviposition inCulex mosquitoes. In laboratory bioassays, significant oviposition responses were obtained fromAe. albopictus, but not fromAe. aegypti, to dilutions of hay infusion and field water. Oviposition responses of both species were moderate to the synthetic compounds tested in the laboratory (0.01–100 µg/liter). Only 3-methylindole (0.1 µg/liter) and 4-ethylphenol (1.0 µg/liter) elicited significantly more oviposition byAe. albopictus than did well water. Of the synthetic compounds tested withAe. aegypti, only phenol (1.0 µg/liter) and 4-ethylphenol (0.1 µg/liter) elicited significantly more oviposition than did well water. Significant repellency or oviposition deterrence for both species occurred in response to at least one high concentration of most of the compounds tested. In field cage evaluations, oviposition responses byAe. albopictus were strongest to larval water and field water, moderate to hay infusion and 3-methylindole (100 µg/liter), and low to well water. A mixture of five synthetic compounds mimicking hay infusion was no more effective than 3-methylindole alone. ForAe. aegypti, oviposition responses were greatest to larval water and least to 3-methylindole. In an olfactometer, gravid females of both species oriented more to field water than to well water and onlyAe. albopictus oriented more to larval water or hay infusion than well water. In general, gravidAe. albopictus responded more strongly to oviposition stimuli than didAe. aegypti.
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  • 5
    Publication Date: 2019-03-28
    Print ISSN: 0021-8561
    Electronic ISSN: 1520-5118
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
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  • 6
    Publication Date: 2020-07-16
    Description: Laboratory assays were conducted to evaluate responses of Diaphorina citri to various aspects of visual cues associated with traps in an effort to improve trap effectiveness. Addition of white or UV violet but not yellow light-emitting diodes (LEDs) increased attraction to standard yellow adhesive traps moderately (11–17%), with no difference in attraction between white or UV violet LEDs. Addition of a black border on yellow traps enhanced collections. However, there were no differences between attraction to black patterns on traps. Comparisons were made between different commercial paints, some with UV-reflecting properties or fluorescence. A yellow paint with UV reflectance, used for painting bird decoys (decoy yellow), was more attractive than the standard yellow Olson sticky trap. Addition of white or green pigment to increase intensity or enhance green reflectance, respectively, did not increase attraction. Alteration of reflectance of Olson traps with addition of UV-reflecting or fluorescent pigments did not enhance attraction of D. citri. In field comparisons, decoy yellow and fluorescent yellow sticky traps were more attractive to D. citri than Olson yellow.
    Electronic ISSN: 2075-4450
    Topics: Biology
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  • 7
    Publication Date: 2016-11-03
    Description: The Asian citrus psyllid (ACP),Diaphorina citriKuwayama, is one of the most serious citrus pests worldwide due to its role as vector of huanglongbing or citrus greening disease. While some optimal plant species for ACP oviposition and development have been identified, little is known of the influence of host plants on ACP size and shape. Our goal was to determine how size and shape of ACP wing and body size varies when development occurs on different host plants in a controlled rearing environment. ACP were reared on six different rutaceous species;Bergera koenigii,Citrus aurantifolia,Citrus macrophylla,Citrus maxima,Citrus taiwanicaandMurraya paniculata. Adults were examined for morphometric variation using traditional and geometric analysis based on 12 traits or landmarks. ACP reared onC. taiwanicawere consistently smaller than those reared on the other plant species. Wing aspect ratio also differed betweenC. maximaandC. taiwanica. Significant differences in shape were detected with those reared onM. paniculatahaving narrower wings than those reared onC. macrophylla. This study provides evidence of wing size and shape differences of ACP based on host plant species which potentially may impact dispersal. Further study is needed to determine if behavioral and physiological differences are associated with the observed phenotypic differences.
    Electronic ISSN: 2167-8359
    Topics: Biology , Medicine
    Published by PeerJ
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  • 8
    Publication Date: 2017-12-13
    Electronic ISSN: 1932-6203
    Topics: Medicine , Natural Sciences in General
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  • 9
  • 10
    Publication Date: 2020-12-08
    Description: Phytophagous insects, including Asian citrus psyllids (Diaphorina citri Kuwayama), use multiple sensory modalities (vision, olfaction, and gustation,) to locate and accept host plants. We explored incorporation of several sensory cues into a multi-modal attract-and-kill device (AK device) using a three-dimensional shape to increase visibility, as well as elements of color, attractant, phagostimulant, UV reflectant, and toxicant. Attraction of adult D. citri to the device was mediated by a combination of a highly reflective yellow cylinder, a UV reflectant compound (magnesium oxide), and an odorant blend as a short-range attractant. The device surface was coated with a slow-release wax matrix (SPLAT™) augmented with a phagostimulant consisting of a 3-component blend (formic acid, acetic acid, and para-cymene) and an insecticide (β-cyfluthrin). Psyllids landing on the device attempted to feed from the wax matrix, became intoxicated, died, and fell from the device. The device remained fully active over a period of 12 weeks partly because dead psyllids or nontargets did not adhere to the surface as occurs on adhesive yellow sticky cards, the industry standard. Laboratory and field assays showed that the device attracted and killed significantly more adult D. citri than ordinary yellow sticky cards. This device or a future iteration based on the design elements of this device is expected to contribute to sustainable and environmentally appropriate management of D. citri by exploiting the psyllid’s innate behavioral responses to visual, olfactory, and gustatory stimuli.
    Electronic ISSN: 2075-4450
    Topics: Biology
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