ISSN:
1573-5168
Keywords:
GnRH
;
salmon GnRH
;
chicken GnRH-II
;
HPLC
;
RIA
;
bluefin tuna
;
red seabream
;
black seabream
;
red spotted grouper
;
Japanese flounder
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Brain extracts from bluefin tuna, Thunnus thynnus, red seabream, Pagrus major, black seabream, Acanthopagrus schlegeli, red spotted grouper, Epinephelus akaara and Japanese flounder, Paralichthys olivaceus, were analyzed by high performance liquid chromatography (HPLC) and specific radioimmunoassays. Immunoreactive material co-eluting from HPLC with salmon gonadotropin-releasing hormone (GnRH) and chicken GnRH-II, respectively, was found in all five species. In addition, a GnRH immunoreactive fraction showing the same HPLC retention time as lamprey GnRH-I was detected in the brain extracts of all species examined when using an unspecific radioimmunoassay which detects several GnRH forms, including lamprey GnRH-I. In the Japanese flounder brain extract, a fourth GnRH immunoreactive fraction was detected with the unspecific radioimmunoassay which did not co-elute with any of the six synthetic GnRH standards used in the present study.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00004418
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