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Chromatographic and immunological identification of gonadotropin-releasing hormone in five marine teleosts

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Abstract

Brain extracts from bluefin tuna, Thunnus thynnus, red seabream, Pagrus major, black seabream, Acanthopagrus schlegeli, red spotted grouper, Epinephelus akaara and Japanese flounder, Paralichthys olivaceus, were analyzed by high performance liquid chromatography (HPLC) and specific radioimmunoassays. Immunoreactive material co-eluting from HPLC with salmon gonadotropin-releasing hormone (GnRH) and chicken GnRH-II, respectively, was found in all five species. In addition, a GnRH immunoreactive fraction showing the same HPLC retention time as lamprey GnRH-I was detected in the brain extracts of all species examined when using an unspecific radioimmunoassay which detects several GnRH forms, including lamprey GnRH-I. In the Japanese flounder brain extract, a fourth GnRH immunoreactive fraction was detected with the unspecific radioimmunoassay which did not co-elute with any of the six synthetic GnRH standards used in the present study.

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Authors and Affiliations

  1. National Research Institute of Aquaculture, Nansei, Watarai, Mie, 516-01, Japan

    Koichi Okuzawa, Hideki Tanaka & Hirohiko Kagawa

  2. Department of Fisheries, Faculty of Agriculture, University of Tokyo, Bunkyo, Tokyo, 113, Japan

    Masafumi Amano & Katsumi Aida

  3. School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada, Aomori, 034, Japan

    Yoshihisa Hasegawa

Authors
  1. Koichi Okuzawa
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  2. Masafumi Amano
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  3. Katsumi Aida
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  4. Yoshihisa Hasegawa
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  5. Hideki Tanaka
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  6. Hirohiko Kagawa
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Okuzawa, K., Amano, M., Aida, K. et al. Chromatographic and immunological identification of gonadotropin-releasing hormone in five marine teleosts. Fish Physiol Biochem 12, 337–345 (1993). https://doi.org/10.1007/BF00004418

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  • DOI: https://doi.org/10.1007/BF00004418

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