Publication Date:
2004-05-29
Description:
Cytokinesis is the essential process that partitions cellular contents into daughter cells. To identify and characterize cytokinesis proteins rapidly, we used a functional proteomic and comparative genomic strategy. Midbodies were isolated from mammalian cells, proteins were identified by multidimensional protein identification technology (MudPIT), and protein function was assessed in Caenorhabditis elegans. Of 172 homologs disrupted by RNA interference, 58% displayed defects in cleavage furrow formation or completion, or germline cytokinesis. Functional dissection of the midbody demonstrated the importance of lipid rafts and vesicle trafficking pathways in cytokinesis, and the utilization of common membrane cytoskeletal components in diverse morphogenetic events in the cleavage furrow, the germline, and neurons.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3679889/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3679889/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Skop, Ahna R -- Liu, Hongbin -- Yates, John 3rd -- Meyer, Barbara J -- Heald, Rebecca -- F32 GM064159/GM/NIGMS NIH HHS/ -- F32 GM064159-01/GM/NIGMS NIH HHS/ -- F32 GM064159-02/GM/NIGMS NIH HHS/ -- F32 GM064159-03/GM/NIGMS NIH HHS/ -- F32 GM64159-01/GM/NIGMS NIH HHS/ -- P41 RR011823/RR/NCRR NIH HHS/ -- RR1823/RR/NCRR NIH HHS/ -- New York, N.Y. -- Science. 2004 Jul 2;305(5680):61-6. Epub 2004 May 27.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, CA 94720, USA. skop@wisc.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/15166316" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
CHO Cells
;
Caenorhabditis elegans/cytology/genetics/physiology
;
Carrier Proteins/analysis/isolation & purification/physiology
;
Cell Cycle/physiology
;
*Cell Division
;
Cell Fractionation
;
Cell Membrane/physiology
;
Computational Biology
;
Cricetinae
;
Cytoskeletal Proteins/analysis/isolation & purification/physiology
;
Cytoskeleton/physiology
;
Germ Cells/physiology
;
HeLa Cells
;
Humans
;
Membrane Microdomains/physiology
;
Morphogenesis
;
Organelles/chemistry/*physiology
;
Protein Transport
;
Proteins/analysis/isolation & purification/*physiology
;
Proteome/*analysis
;
Proteomics
;
Signal Transduction
;
Spindle Apparatus/physiology/ultrastructure
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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