Publication Date:
2002-11-16
Description:
Activation-induced cytidine deaminase (AID) plays an essential role in class switch recombination (CSR) and somatic hypermutation (SHM) of immunoglobulin genes. We report here that deficiency in AID results in the development of hyperplasia of isolated lymphoid follicles (ILFs) associated with a 100-fold expansion of anaerobic flora in the small intestine. Reduction of bacterial flora by antibiotic treatment of AID-/- mice abolished ILF hyperplasia as well as the germinal center enlargement seen in secondary lymphoid tissues. Because an inability to switch to immunoglobulin A on its own does not lead to a similar phenotype, these results suggest that SHM of ILF B cells plays a critical role in regulating intestinal microflora.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Fagarasan, Sidonia -- Muramatsu, Masamichi -- Suzuki, Keiichiro -- Nagaoka, Hitoshi -- Hiai, Hiroshi -- Honjo, Tasuku -- New York, N.Y. -- Science. 2002 Nov 15;298(5597):1424-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Medical Chemistry, Graduate School of Medicine, Kyoto University, Yoshida, Sakyo-ku, Kyoto 606-8501, Japan.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/12434060" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Anti-Bacterial Agents
;
B-Lymphocytes/immunology
;
Bacteria, Aerobic/*growth & development
;
Bacteria, Anaerobic/*growth & development
;
Cell Division
;
Colony Count, Microbial
;
Cytidine Deaminase/genetics/*metabolism
;
Dendritic Cells, Follicular/immunology
;
Drug Therapy, Combination/pharmacology
;
Genes, Immunoglobulin
;
Germinal Center/immunology
;
Homeostasis
;
Hyperplasia
;
Immunization
;
Immunoglobulin Class Switching
;
Immunoglobulin Variable Region/genetics
;
Intestine, Small/immunology/*microbiology
;
Lymphocyte Activation
;
Lymphoid Tissue/immunology/*pathology
;
Metronidazole/pharmacology
;
Mice
;
Mice, Inbred C57BL
;
Mice, Inbred CBA
;
Peyer's Patches/pathology
;
Somatic Hypermutation, Immunoglobulin
;
T-Lymphocytes/immunology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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