ISSN:
1432-072X
Keywords:
l-Azetidine 2-carboxylic acid
;
General amino-acid permease
;
Amino-acid transport
;
Protein synthesis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Addition of the l-proline analogue l-azetidine 2-carboxylic acid to growing cultures of Saccharomyces cerevisiae var. ellipsoideus promoted fast deactivation of the general aminoacid permease, measured as l-valine uptake, without an immediate decrease in the growth rate. Cells preincubated with the analogue for 3 h were unable to restore either growth ability or general aminoacid permease activity in analogue-free medium. Eadie-Hofstee plots of l-valine uptake in the presence of the analogue are consistent with a strong reduction in the number of active molecules of the general amino-acid permease located in the plasma membrane. Inhibitory effects on protein synthesis were seen after preincubations of the yeast with the analogue for 3 h although a 30 min preincubation had no effect.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00243449
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