Abstract
Addition of the l-proline analogue l-azetidine 2-carboxylic acid to growing cultures of Saccharomyces cerevisiae var. ellipsoideus promoted fast deactivation of the general aminoacid permease, measured as l-valine uptake, without an immediate decrease in the growth rate. Cells preincubated with the analogue for 3 h were unable to restore either growth ability or general aminoacid permease activity in analogue-free medium. Eadie-Hofstee plots of l-valine uptake in the presence of the analogue are consistent with a strong reduction in the number of active molecules of the general amino-acid permease located in the plasma membrane. Inhibitory effects on protein synthesis were seen after preincubations of the yeast with the analogue for 3 h although a 30 min preincubation had no effect.
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Abbreviations
- GAP:
-
general amino-acid permease
- AZC:
-
l-azetidine 2-carboxylic acid
- YNB:
-
yeast nitrogen base
- YE:
-
Yeast extract
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Iglesias, R., Ferreras, J.M., Arias, F.J. et al. Effect of l-azetidine 2-carboxilic acid on the activity of the general amino-acid permease from Saccharomyces cerevisiae var. ellipsoideus . Arch. Microbiol. 155, 320–324 (1991). https://doi.org/10.1007/BF00243449
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DOI: https://doi.org/10.1007/BF00243449