Abstract
Addition of the l-proline analogue l-azetidine 2-carboxylic acid to growing cultures of Saccharomyces cerevisiae var. ellipsoideus promoted fast deactivation of the general aminoacid permease, measured as l-valine uptake, without an immediate decrease in the growth rate. Cells preincubated with the analogue for 3 h were unable to restore either growth ability or general aminoacid permease activity in analogue-free medium. Eadie-Hofstee plots of l-valine uptake in the presence of the analogue are consistent with a strong reduction in the number of active molecules of the general amino-acid permease located in the plasma membrane. Inhibitory effects on protein synthesis were seen after preincubations of the yeast with the analogue for 3 h although a 30 min preincubation had no effect.
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Abbreviations
- GAP:
-
general amino-acid permease
- AZC:
-
l-azetidine 2-carboxylic acid
- YNB:
-
yeast nitrogen base
- YE:
-
Yeast extract
References
Dubois E, Grenson M (1979) Methylamine/Ammonia Uptake Systems in Saccharomyces cerevisiae: Multiplicity and Regulation. Mol. Gen Genet 175: 67–76
Eddy AA (1982) Mechanisms of solute transport in selected eukaryotic microorganisms. Adv Microb Physiol 24: 1–78
Ferreras JM, Iglesias R, Girbés T (1989) Effect of the chronic ethanol action on the activity of the general amino acid permease from Saccharomyces cerevisiae var. ellipsoideus. Biochim Biophys Acta 979: 375–377
Ferreras JM, Iglesias R, Muñoz R, Arias FJ, Girbés T (1990) Influence of the structure of alkanols on their inhibition of protein synthesis in Saccharomyces cerevisiae var. ellipsoideus. Cell Mol Biol 36: 337–344
Girbés T, Parrilla R (1983) Effect of ethanol on the uptake and utilization of nutrients by alcohol-resistant yeasts. Cell Mol Biol 29: 443–449
Girbés T, Parrilla R (1986) Correlation between ethanol resistance and incorporation of nutrients by must fermenting yeast. Cell Mol Biol 32: 545–549
Girbés T, Susin A, Ayuso MS, Parrilla R (1983) Acute effects of ethanol in the control of protein synthesis. Arch Biochem Biophys 226: 37–49
Grenson M, Hou C (1972) Ammonia inhibition of the general amino acid permease and its suppression in NADPH-specific glutamate dehydrogenaseless mutants of Saccharomyces cerevisiae. Biochem Biophys Res Commun 48: 749–756
Leao C, Van Uden N (1984) Effects of ethanol and other alkanols on the general amino acid permease of Saccharomyces cerevisiae. Biotechnol Bioeng 26: 403–405
Mizzen LA, Welch WJ (1988) Characterization of the thermotolerant cell. I. Effects on protein synthesis activity and the regulation of heat-shock protein to expression. J Cell Biol 106: 1105–1116
Rytka J (1975) Positive selection of general amino acid permease mutants in Saccharomyces cerevisiae. J Bacteriol 121: 562–570
Wiame JM, Grenson M, Arst Jr HN (1985) Nitrogen catabolite repression in yeasts and filamentous fungi. Adv Microb Physiol 26: 1–88
Woodward JR, Kornberg HL (1981) Changes in membrane proteins associated with inhibition of the general amino acid permease of yeast (Saccharomyces cerevisiae). Biochem J 196: 531–536
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Iglesias, R., Ferreras, J.M., Arias, F.J. et al. Effect of l-azetidine 2-carboxilic acid on the activity of the general amino-acid permease from Saccharomyces cerevisiae var. ellipsoideus . Arch. Microbiol. 155, 320–324 (1991). https://doi.org/10.1007/BF00243449
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DOI: https://doi.org/10.1007/BF00243449