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  • 1
    Publication Date: 2021-05-19
    Description: Aquaculture of sturgeon species and their hybrids is being considered as an important substitution for sturgeon catch due to highly decrease of natural populations, artificial propagation and fingerling release in the sea. In this study, big bester, a new hybrid sturgeon (female beluga × male bester) was produced for the first time in IRAN. Sperm of 7350 ± 1682 kg male bester was used to fertilize the eggs of one 54 kg female Huso huso. The fries of big bester and control trestment of beluga were fed by artificial concentrated food (48-50% protein and 15-17% fat) after egg yolk absorbance, a period of feeding on Artemia and Daphnia. Results showed that rearing and feeding of bester broods was efficient to reach the fish to maturation stage and there is an opportunity to collect qualified ova and sperm from F1 generation. Meanwhile sex determination and maturity assessment of gonads were successfully done via laparoscopy method. The comparison of produced big bester fingerlings with control beluga fingerling showed that the weigth of big bester fingerlings has not significant difference with beluga's (p〈 0.05) at the age up to 2 months.At 3 months of age, beluga fingerlings showed higher weight (p〉0.05), but there are faster growth rate in big bester fingerlings from 3 months of age up to 5 months (p〉 0.05) in comparison with belugs fingerlings. Meanwhile no statistically significant difference was found between length of big bester and beluga fingerlings among any age. The results of current study showed the potential of rearing male bester to produce matured broods and collection of their sperm for big bester production.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Beluga ; Bester ; Hybridization ; Big bester ; Aquaculture ; Productions
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 44pp.
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  • 2
    Publication Date: 2021-05-19
    Description: This study was conducted to codify the guideline and indexes of broodstock management in salmonid fish and cryopreservation, selective breeding and sex reversal in hatchery to attain responsible aquaculture development. Cryopreservation includes of sperm and egg in term of economic is important in aquaculture. In this report mentioned the type and name of material. All female and sex reversal fish are produced by steroid hormone which mentioned in this report. Selective breeding include individual selection and family selection which could be used for genetic improvement of fish.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Economic ; Broodstock ; Reproduction ; Cryopreservation ; Genetic ; Breeding ; Aquaculture ; Salmonid fish ; Sex reversal ; Hatchery ; Sperm ; Egg ; Female ; Steroid hormones
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 46pp.
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  • 3
    Publication Date: 2021-05-19
    Description: Location of family Satellite DNA (HindIII SatDNA) on chromosomes of Acipenser persicus and Acipenser gueldenstaedtii was analyzed using Fluorescent In Situ Hybridization (FISH) technique to determine the genetic differences between these two species. After obtaining suitable metaphase plates using leukocyte culture and HindIII SatDNA extraction from genome of the fish under study, extracted SatDNA from genome of A. gueldenstaedtii was used as a FISH probe. The probe was labeled with spectrum orange (Orange-dUTP) and hybridized with chromosomes of the fish under study. Analysis of SatDNA sequences isolated from A. persicus and A. gueldenstaedtii showed the presence of 163bp for A. persicus and 168bp for A. gueldenstaedtii. In the study of metaphase plates 66±4 signals were detected from the hybridization of the probe used with chromosomes of A. persicus and 68±3 signals were detected from the hybridization of the probe with chromosomes of A. gueldenstaedtii. Due to presence of numerous microchromosomes and heterogenous and large hybridization signals, it was impossible to identify the precise position of signals on chromosomes. The following results are worth mentioning: 1. Developing leukocyte culture method for A. gueldenstaedtii and completing it for all of the Caspian Sea sturgeons. 2. Developing a suitable culture medium for the Caspian Sea sturgeons. 3. Isolating sturgeons HindIII Satellite DNA family from A. persicus and A. gueldenstaedtii, determining sequences and registering the sequences in the NCBI gene bank (FJ429174; FJ 94465). 4. Developing a protocol for FISH technique in A. persicus and A. gueldenstaedtii which has opened a new era in aquaculture genetic studies entitled Molecular cytogenetics in the country. Considering its potential applications, this technique can be applied to other aquatic species.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Acipenser persicus ; Acipenser gueldenstaedtii ; Fluorescent In Situ Hybridization ; HindIII SatDNA ; Molecular cytogenetics technique ; Chromosome ; Genetic ; Species ; Aquatic
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 68pp.
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