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  • 1
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    Early behavioral plasticity: perinatal development (1988)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1988-06-03
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Nelson, P G -- New York, N.Y. -- Science. 1988 Jun 3;240(4857):1351-2.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/17815857" target="_blank"〉PubMed〈/a〉
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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  • 2
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    Synaptic connections in vitro: modulation of number and efficacy by electrical activity (1989)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1989-05-05
    Description: The functional architecture of synaptic circuits is determined to a crucial degree by the patterns of electrical activity that occur during development. Studies with an in vitro preparation of mammalian sensory neurons projecting to ventral spinal cord neurons slow that electrical activity induces competitive processes that regulate synaptic efficacy so as to favor activated pathways over inactive convergent pathways. At the same time, electrical activity initiates noncompetitive processes that increase the number of axonal connections between these sensory and spinal cord neurons.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Nelson, P G -- Yu, C -- Fields, R D -- Neale, E A -- New York, N.Y. -- Science. 1989 May 5;244(4904):585-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Laboratory of Developmental Neurobiology, National Institute of Child Health and Human Development, Bethesda, MD 20892.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2717942" target="_blank"〉PubMed〈/a〉
    Keywords: Action Potentials ; Afferent Pathways/physiology ; Animals ; Axons/physiology ; Electric Stimulation ; Ganglia, Spinal/cytology ; Mice ; Neurons/*physiology ; Neurons, Afferent/*physiology ; Spinal Cord/*cytology/embryology ; Synapses/*physiology
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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  • 3
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    Specific-opiate-induced depression of transmitter release from dorsal root ganglion cells in culture (1978)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1978-03-31
    Description: The opiate etorphine depresses monosynaptic excitatory postsynaptic potentials (EPSP's) elicited in spinal cord cells by activation of dorsal root ganglion cells in murine neuronal cell culture. The depression is reversed by naloxone. Statistical analysis of the synaptic responses reveals that the opiate reduces EPSP quantal content at this synapse without altering quantal size. Therefore, the opiate action is presynaptic and affects transmitter release rather than postsynaptic responsiveness.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Macdonald, R L -- Nelson, P G -- New York, N.Y. -- Science. 1978 Mar 31;199(4336):1449-51.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/204015" target="_blank"〉PubMed〈/a〉
    Keywords: Cells, Cultured ; Depression, Chemical ; Dose-Response Relationship, Drug ; Etorphine/*pharmacology ; Ganglia, Spinal/*drug effects ; Membrane Potentials/drug effects ; Morphinans/*pharmacology ; Naloxone/pharmacology ; Nerve Endings/drug effects ; Spinal Cord/drug effects ; Synapses/drug effects ; Synaptic Transmission/*drug effects
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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  • 4
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    Axonal proteins of presynaptic neurons during synaptogenesis (1983)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1983-09-23
    Description: Changes occur in the synthesis and axonal transport of neuronal proteins in dorsal-root ganglia axons as a result of contact with cells from the spinal cord during synapse formation. Dorsal-root ganglia cells were cultured in a compartmental cel culture system that allows separate access to neuronal cell bodies and their axons. When cells from the ventral spinal cord were cultured with the dorsal-root ganglia axons, synapses were established within a few days. Metabolic labeling and two-dimensional electrophoresis revealed that four of more than 300 axonal proteins had changed in their expression by the time synapses were established. The highly selective nature of these changes suggests that the proteins involved may be important in the processes of axon growth and synapse formation and their regulation by the regional environment.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Sonderegger, P -- Fishman, M C -- Bokoum, M -- Bauer, H C -- Nelson, P G -- New York, N.Y. -- Science. 1983 Sep 23;221(4617):1294-7.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6612344" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Axons/*metabolism ; Cells, Cultured ; Chick Embryo ; Isoelectric Point ; Molecular Weight ; Nerve Tissue Proteins/*biosynthesis ; Synapses/*metabolism
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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  • 5
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    A coeruleo-spinal system in culture (1981)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1981-07-17
    Description: In combined cultures of dissociated spinal neurons and explants from the region of locus coeruleus, rich catecholamine-containing fiber projections from the explant to the surrounding regions of spinal neurons were demonstrated by fluorescence histochemistry. Electrical stimulation of the explant resulted in slow depolarizing responses in many of the spinal neurons. Cells exhibiting this type of response were also usually depolarized by local application of noradrenaline, whereas other, unresponsive neurons usually were not. The depolarizing responses to electrical stimulation and to noradrenaline were both increased by depolarizing current injection and decreased by hyperpolarizing current. These and other data suggest that the depolarizing responses of the spinal neurons to explant stimulation are mediated by noradrenaline released from axons of locus coeruleus neurons.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Marshall, K C -- Pun, R Y -- Hendelman, W J -- Nelson, P G -- New York, N.Y. -- Science. 1981 Jul 17;213(4505):355-7.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7244621" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Animals, Newborn ; Desipramine/pharmacology ; Locus Coeruleus/cytology/drug effects/*physiology ; Membrane Potentials/drug effects ; Mice ; Microscopy, Fluorescence ; Neurons/physiology ; Norepinephrine/pharmacology ; Organ Culture Techniques ; Spinal Cord/cytology/drug effects/*physiology
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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  • 6
    Electronic Resource
    Electronic Resource
    An ultra-low-noise, low-frequency, six degrees of freedom active vibration isolator (1997)
    [S.l.] : American Institute of Physics (AIP)
    Review of Scientific Instruments 68 (1997), S. 3211-3219 
    Details
    ISSN: 1089-7623
    Source: AIP Digital Archive
    Topics: Physics , Electrical Engineering, Measurement and Control Technology
    Notes: To extend the operating frequency band of earth-based interferometric gravitational wave (GW) detectors down to 1 Hz, an unconventional system is required that provides approximately a factor of one million vibration isolation for the horizontal and more for the vertical, starting at 1 Hz. To this end, a three-stage ultra-low-noise, high-performance active and passive isolation system is being designed and constructed in order to demonstrate that a high degree of reduction can be achieved for vibrations at the support points of a GW detector's final pendulum system. The first stage of this system has been fully characterized and is the main subject of this article. It is an equilateral triangular platform, 1.1 m on a side, with a total mass of 460 kg, including the vacuum system that will contain the other two stages. Active isolation is achieved by six servocontrol loops, using signals from low-frequency displacement sensors to feed back to noncontacting force transducers. The first stage has been actively isolated from vibrations in all six degrees of freedom by at least a factor of 100 at 1 Hz and above. The system noise limitations at 2 Hz and above are roughly 2×10−11 and 5×10−11 m/Hz for vertical and horizontal translations, respectively. © 1997 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.1148269
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  • 7
    Electronic Resource
    Electronic Resource
    Electrogenesis in mouse neuroblastoma cells In vitro (1971)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 77 (1971), S. 337-352 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Intracellular microelectrode studies of passive membrane properties and action potential generation were carried out on cloned and uncloned mouse neuroblastoma cells in tissue culture. The cloned cells were studied between the eighth and tenth months and the uncloned cells between the third and fifth weeks after primary dissociation. Electrophysiologic measurements of cell membrane properties were made by passing stimulating current pulses across the cell membrane from an intracellular microelectrode and recording simultaneously from the same electrode, by means of a bridge circuit, the changes in membrane potential. The range of responses to electrical stimulation varied from passive increases in membrane potential to repetitive firing of action potentials. A 20 fold range in spike generating capability was found. Passive membrane properties (membrane potential, specific membrane resistivity, and specific membrane capacitance) were similar to those of sympathetic neurons in intact preparations. Seventy-nine percent of the cloned cell line compared to 94% of the uncloned line were capable of generating action potentials. Less than 2% of the cloned cells showed repetitive firing whereas 23% of the uncloned cells had this property. As in several types of normal neurons, the action potential mechanism was largely, although not completely, blocked by iontophoretic and bath applied tetrodotoxin.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040770308
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  • 8
    Electronic Resource
    Electronic Resource
    Responses of neuroblastoma cells to iontophoretically applied acetylcholine (1971)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 77 (1971), S. 353-362 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Dissociated mouse neuroblastoma cells were studied in vitro by using intracellular microelectrodes for electrical stimulation and recording. Some, but not all cells, which exhibited well developed action potentials to electrical stimulation also showed changes in membrane potential to iontophoretically applied acetylcholine (ACh). The types of responses to ACh varied. Short latency depolarizing responses to pulses of ACh (similar to those obtained with skeletal muscle) as well as sustained depolarization to steady ACh application (D response) occurred. A longer latency prolonged hyperpolarizing response (H response) and bi- and triphasic combinations of H and D responses were also seen.Pairs of cells showing morphologic contact were tested for the occurrence of effective synaptic coupling by placing intracellular microelectrodes in each cell. In none of 95 cases tested did spike activity produced by direct electrical stimulation of one cell elicit a synaptic potential of 200 μv or more in the other.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040770309
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  • 9
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    Cerebral cortical astroglia from the trisomy 16 mouse, a model for Down syndrome, produce neuronal cholinergic deficits in cell culture (1997)
    National Academy of Sciences
    Details
    Publication Date: 1997-11-11
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
    Published by National Academy of Sciences
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  • 10
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    An ultra-low-noise, low-frequency, six degrees of freedom active vibration isolator (1997)
    American Institute of Physics
    Details
    Publication Date: 1997-08-01
    Print ISSN: 0034-6748
    Electronic ISSN: 1089-7623
    Topics: Electrical Engineering, Measurement and Control Technology , Physics
    Published by American Institute of Physics
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