Publikationsdatum:
2007-02-01
Beschreibung:
HIV-1 Nef is a small protein (approx. 25 kDa) that is posttranslationally modified by myristoylation. To explain its complex activities, a ‘Nef-cycle’ is discussed, which postulates different molecular conformations of Nef. Using recombinant full-length non-myristoylated Nef and synthetic peptides, we demonstrate by fluorescence titration experiments that a peptide representing the myristoylated N-terminus of Nef is specifically bound by Nef. A non-myristoylated N-terminal fragment of Nef or a myristoylated control peptide does not bind to Nef. These results are the first direct experimental evidence of the existence of a myristate-binding pocket in Nef, a prerequisite of the postulated ‘closed’ Nef conformation.
Print ISSN:
1431-6730
Digitale ISSN:
1437-4315
Thema:
Biologie
,
Chemie und Pharmazie
Permalink