Publication Date:
1992-10-19
Description:
The development of high brightness and short pulse width (〈 200 picoseconds) x-ray lasers now offers biologists the possibility of high-resolution imaging of specimens in an aqueous environment without the blurring effects associated with natural motions and chemical erosion. As a step toward developing the capabilities of this type of x-ray microscopy, a tantalum x-ray laser at 44.83 angstrom wavelength was used together with an x-ray zone plate lens to image both unlabeled and selectively gold-labeled dried rat sperm nuclei. The observed images show approximately 500 angstrom features, illustrate the importance of x-ray microscopy in determining chemical composition, and provide information about the uniformity of sperm chromatin organization and the extent of sperm chromatin hydration.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Da Silva, L B -- Trebes, J E -- Balhorn, R -- Mrowka, S -- Anderson, E -- Attwood, D T -- Barbee, T W Jr -- Brase, J -- Corzett, M -- Gray, J -- New York, N.Y. -- Science. 1992 Oct 9;258(5080):269-71.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Physics, University of California, Berkeley 94720.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/1411525" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Cell Fractionation
;
Cell Nucleus/*ultrastructure
;
Chromatin/ultrastructure
;
DNA/ultrastructure
;
Epididymis/cytology
;
Immunohistochemistry
;
*Lasers
;
Male
;
Microscopy/*methods
;
Rats
;
Spermatozoa/*ultrastructure
;
X-Rays
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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