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  • 1
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    The systemic delivery of an oncolytic adenovirus expressing decorin inhibits bone metastasis in a mouse model of human prostate cancer (2014)
    Springer Nature
    Details
    Publication Date: 2014-12-11
    Print ISSN: 0969-7128
    Electronic ISSN: 1476-5462
    Topics: Biology , Medicine
    Published by Springer Nature
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    PAPER CURRENT
  • 2
    Electronic Resource
    Electronic Resource
    Two additive QTLs conferring broad-spectrum resistance in potato to Globodera pallida are localized on resistance gene clusters (2000)
    Springer
    Theoretical and applied genetics 101 (2000), S. 1122-1130 
    Details
    ISSN: 1432-2242
    Keywords: Key words AFLP ; CAPS ; G. rostochiensis ; Solanum tuberosum ; QTL mapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Broad-spectrum resistance in potato to the potato cyst nematode (PCN) is commonly regarded as a complex inherited trait. Yet, in this paper we show that, by use of a selected set of PCN test populations, broad-spectrum resistance to the species Globodera pallida can be fully ascribed to the action of two loci: Gpa5 and Gpa6. These loci were readily mapped by means of a strategy based on two steps. Firstly, the chromosomal localization of both loci was assessed by use of an online catalogue of AFLP markers covering a substantial part of the potato genome (http://www.spg.wau.nl/pv/aflp/catalog.htm). Subsequently the chromosomal regions of both loci were identified by means of CAPS markers based on RFLP insert sequences. Locus Gpa5 explains at least 61% of the genetic variation. This locus maps to chromosome 5 on a region which has previously been shown to harbor resistance factors to viral (Nb, Rx2), fungal (R1) and nematodal (Gpa, Grp1) pathogens. The Gpa6 locus exhibits a minor effect on the resistance (24%) and acts additively to Gpa5. Interestingly, the Gpa6 locus maps to a region on chromosome 9 where, in the homoeologous tomato genome, the virus resistance gene Sw-5 resides as part of a resistance gene cluster. In potato, resistance to potato virus X has been reported in the vicinity of this region. The map location of Gpa6 indicates the presence of a resistance gene cluster at the end of the long arm of chromosome 9 of potato.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051588
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  • 3
    Electronic Resource
    Electronic Resource
    Comparative performance of FDR and SDR progenies from reciprocal 4x-2x crosses in potato (1994)
    Springer
    Theoretical and applied genetics 89 (1994), S. 545-550 
    Details
    ISSN: 1432-2242
    Keywords: First division restitution ; Second division restitution ; Reciprocal 4x-2x crosses ; Solanum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Numerically unreduced (2n) gametes from first division restitution (FDR) are considered to be superior to 2n-gametes from second division restitution (SDR) because they transfer a larger proportion of the total parental heterozygosity and epistasis intact to the tetraploid progeny. This supposed superiority was investigated by comparing 12 sets of reciprocal 4x-2x crosses. Each diploid parent used in a reciprocal set produced 2n-pollen by FDR and 2n-eggs by SDR. Six agronomic characters were investigated. FDR progenies (from 4x.2x) were found to have higher mean yields due to more and bigger tubers. With respect to underwater weight, the overall progeny mean of FDR progenies was significantly higher than that of SDR progenies (from 2x.4x). However, the absolute difference found between both overall progeny means was too small to be of practical significance. No differences between FDR and SDR progeny means were found for vine maturity and chip colour. In addition to the progeny mean, within-progeny variation is important in potato breeding. For vine maturity a higher within-progeny variation was detected in SDR progenies, whereas within-progeny variations for yield, underwater weight and chip colour were not different in FDR and SDR progenies. With regard to vine maturity, we conclude that SDR 2n-gametes are superior to FDR 2n-gametes because, with the same progeny means of FDR and SDR progenies, the within-progeny variation was higher in SDR progenies. Therefore the assumed superiority of FDR 2n-gametes was confirmed for yield but was not observed for vine maturity, underwater weight and chip colour.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222446
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  • 4
    Electronic Resource
    Electronic Resource
    Mapping of the cyst nematode resistance locus Gpa2 in potato using a strategy based on comigrating AFLP markers (1997)
    Springer
    Theoretical and applied genetics 95 (1997), S. 874-880 
    Details
    ISSN: 1432-2242
    Keywords: Key words Gene-for-gene ; Globodera pallida ; G. rostochiensis ; Potato Virus X ; Solanum tuberosum ssp. andigena CPC 1673
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The nematode resistance locus Gpa2 was mapped on chromosome 12 of potato using information on the genomic positions of 733 known AFLP markers. The minimum number of AFLP primer combinations required to map Gpa2 was three. This demonstrates that a reference collection of potato AFLP markers may be a valuable tool for mapping studies in potato. By use of RFLP probes, Gpa2 was more precisely mapped at the distal end of chromosome 12. Gpa2 confers resistance to a distinct group of populations of the potato cyst nematode Globodera pallida and originates from the same potato accession as locus H1, conferring resistance to pathotype Ro1 of G. rostochiensis. This study shows that these two nematode resistance loci are unlinked and that Gpa2 is linked to the Rx1 locus conferring resistance to potato virus X. The efficiency of AFLPs for genetic mapping of a highly heterozygous crop like potato is discussed and compared with the RFLP technique.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050638
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  • 5
    Electronic Resource
    Electronic Resource
    A QTL for broad-spectrum resistance to cyst nematode species (Globodera spp.) maps to a resistance gene cluster in potato (1998)
    Springer
    Theoretical and applied genetics 96 (1998), S. 654-661 
    Details
    ISSN: 1432-2242
    Keywords: Key words AFLP ; CAPS ; G. pallida ; G. rostochiensis ; Solanum ; QTL mapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Broad-spectrum resistance in potato to the potato cyst nematode (PCN) species Globodera rostochiensis and G. pallida is commonly regarded as a polygenically inherited trait. Yet, by use of QTL analysis and a selected set of PCN populations, resistance to both PCN species could be ascribed to the action of locus Grp1. Grp1 confers major resistance to G. rostochiensis line Ro5-22 and G. pallida population Pa2-D383 and partial resistance to G. pallida population Pa3-Rookmaker. Grp1 was mapped on chromosome 5 using previously characterized AFLP markers. Cleaved amplified polymorphic sequence (CAPS) markers available for RFLP loci GP21 and GP179 revealed that Grp1 maps on a genomic region harboring other resistance factors to viral, fungal and nematodal pathogens. The present data indicate that Grp1 is a compound locus which contains multiple genes involved in PCN resistance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050785
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  • 6
    Electronic Resource
    Electronic Resource
    Evaluation of dihaploid populations from potato varieties and breeding lines (1995)
    Springer
    Potato research 38 (1995), S. 77-86 
    Details
    ISSN: 1871-4528
    Keywords: 2n-pollen ; Globodera rostochiensis Woll ; flowering behaviour ; mutants ; pollen stainability ; Solanum tuberosum L ; tuberization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Dihaploid populations from 20 varieties and 11 breeding lines ofSolanum tuberosum differed greatly in the occurrence and frequency of mutant phenotypes, in tuberization, flowering, pollen stainability, 2n-pollen production and resistance toGlobodera rostochiensis pathotype Ro-1. One population contained up to 61.7% lethal mutants: 39.1% of the total of 5377 dihaploids obtained did not tuberize, and 32.4% of 825 vigorous dihaploids did not flower. Of 825 vigorous dihaploids, 26.9 and 3.9% respectively had a pollen stainability higher than 20 and 60%. Among 222 dihaploids with a pollen stainability higher than 20%. 23.0% produced some 2n-pollen and none of these had a stainability higher than 60%. Among the dihaploid populations investigated the proportion of resistant to susceptible dihaploids ranged from 8:26 to 62:8.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02358072
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  • 7
    Electronic Resource
    Electronic Resource
    Use of allele specificity of comigrating AFLP markers to align genetic maps from different potato genotypes (1997)
    Springer
    Molecular genetics and genomics 255 (1997), S. 438-447 
    Details
    ISSN: 1617-4623
    Keywords: Key words Comparative mapping ; DNA markers ; Solanum tuberosum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The allele specificity of AFLP markers was assessed in five relatively unrelated potato genotypes. To this end, two diploid mapping populations of potato, F1SH × RH and F1AM × RH, were analysed using four and six AFLP primer combinations, respectively, recently applied to the analysis of the genetically well characterized backcross population BC_C × E. The AFLP profiles of the five parents revealed 733 AFLP markers and, when identical primer combinations were used, 131 comigrating AFLP markers were identified. After construction of five parental maps, the genomic positions of these comigrating AFLP markers were compared and 117 markers (89%) which targeted the same genomic region were assumed to be homologous. Of these putative homologues, 20 markers, each cloned from at least two genotypes, were sequenced and 19 sets of amplification products were shown to be nearly identical. The number of AFLP markers previously mapped in population BC_C × E ranged from three to eleven per chromosome, which allowed a reliable assessment of chromosome numbers from individual linkage groups obtained in populations F1SH × RH and F1AM × RH. The high incidence of corresponding AFLP alleles was confirmed by using an additional set of five primer combinations. The 733 AFLP markers localized provide a valuable reference collection for future mapping studies in potato. As a consequence AFLP analysis may replace more laborious locus-specific marker techniques.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004380050516
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  • 8
    Electronic Resource
    Electronic Resource
    Analysis of parental effects on mean vine maturity and chip colour of 4x.2x potato progenies (1996)
    Springer
    Euphytica 88 (1996), S. 175-179 
    Details
    ISSN: 1573-5060
    Keywords: chip colour ; diploids ; sexual polyploidization ; Solanum ; vine maturity ; potato
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The parental effects on progenies from potato 4x.2x(FDR) crosses were analyzed to determine anether and to what extent selection criteria used for selecting tetraploid breeding lines have to be adjusted when selecting diploid breeding lines. For vine maturity and chip colour multiple regression analysis of progeny means on means of diploid and tetraploid parents revealed high coefficients of determination (R2) for both characters, indicating good predicting power of the performance of both diploid and tetraploid parents on the performance of their 4x.2x progenies. For vine maturity the multiple regression slope for the tetraploid perents was significantly larger than for the diploid parents, and progeny means were towards the tetraploid parents. This indicates that selection criteria for vine maturity may be less stringent at the diploid level. However, diploids may be earlier maturing than tetraploids and therefore selection criteria can better be similar at both ploidy levels. For chip colour, multiple regression slopes for diploid and tetraploid parents were similar and progeny means were towards the darkest coloured parent irrespective of its ploidy level. This indicates that selection of breeding lines for chip colour can be conducted similarly at both ploidy levels.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023888
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  • 9
    Electronic Resource
    Electronic Resource
    Analysis of dihaploid induction and production ability and seed parent x pollinator interaction in potato (1993)
    Springer
    Euphytica 72 (1993), S. 61-64 
    Details
    ISSN: 1573-5060
    Keywords: dihaploid potato induction ; pseudogamy ; Solanum tuberosum L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The production of dihaploids is the first step in a potato breeding program at the diploid level. Dihaploid induction ability, dihaploid production ability and seed parent x pollinator interaction were analyzed for 28 × 3, seed parent x pollinator combinations. This is the first report on significant interaction between seed parents and pollinators. Despite this interaction, IVP 101 had a significantly higher dihaploid induction ability than the widely used pollinators IVP 35 and IVP 48. Previous findings on significant differences in dihaploid production ability between seed parents were clearly confirmed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023773
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  • 10
    Electronic Resource
    Electronic Resource
    Comparative performance of diploid and tetraploid progenies from 2x.2x crosses in potato (1995)
    Springer
    Euphytica 81 (1995), S. 187-192 
    Details
    ISSN: 1573-5060
    Keywords: chip colour ; diploids ; Solanum tuberosum ; under water weight ; vine maturity ; yield ; yield components
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Selection criteria for agronomic characters in a potato breeding program at the diploid (2x) level may differ from selection criteria used when selecting breeding lines at the tetraploid (4x) level. Differences between selection criteria are expected, (1) when expression of the characters is different at both ploidy levels and/or (2) when the effect of diploid breeding lines on agronomic characters of tetraploid progenies is different from the effect of tetraploid breeding lines. In this investigation sets of diploid and tetraploid progenies, each set derived from the same 2x.2x cross, were compared as to the expression of six agronomic characters. Diploid progenies had significantly lower yields (due to smaller tubers) and significantly higher under water weights than tetraploid progenies. Vine maturity and chip colour were similarly expressed at both ploidy levels. Correlations between yield and yield components, and between under water weight and chip colour were similar at both ploidy levels. The lower yields and higher under water weights found in diploids point to the need of different selection criteria for selecting diploid and tetraploid breeding lines.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00025432
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