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  • 1
    Publication Date: 2016-02-01
    Print ISSN: 0378-1119
    Electronic ISSN: 1879-0038
    Topics: Biology
    Published by Elsevier
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  • 2
    Publication Date: 2021-05-19
    Description: In this study lusiferse gene extracted from Vibrio fischeri. V. fischeri strain was kindly provided by Iranian Research Organization for Science and Technology (IROST). Genomic DNA extraction was carried out by Phenol-chloroform. A DNA fragment encoding the luxA and LuxB was amplified by PCR using sense and antisense primers, specific restriction sites for BamH1 and Kpn1 were introduced into 5´ end of forward and reverse primer, respectively. The PCR product was purified from agarose gel and ligated into cleaved PT257R cloning vector. Following the confirmation of the cloned Luciferase transformed into E. coli. Recombinant clones were confirmed by specific PCR and restriction enzyme digestion analysis. The luxA and LuxB fragment was released subcloned in to the PcDNA3.1\hug and PcDNA3.1\neo expression vectors, respectively. recombinant plasmid was confirmed through restriction digestion using BamH1 and Kpn1 enzymes and subsequently, transformation procedure continued into NIH3T3 eukaryote cells by specific kit. Luminescence ability of recombinant clones was tested by NIH3T3 cells and dechanal (substrate) and Neomysin and hygromysin. The results showed that luminescence start after 2 hours and then increase after 6 hours. Inaddition, the protein identity was verified by western blot analysis, the protein bands 76 kD were detected. , which indicates protein expression of luxB, luxA.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Lusifrase ; Vibrio fischeri ; Transfer gene ; DNA ; Lusiferase gene ; Investihation ; Genetic ; Cyprins carpio ; Rutilus frisii kutum
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 58pp.
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  • 3
    Publication Date: 2021-05-19
    Description: The Common carp is the most important culture fish in the word, that can be consider as domestic fish. The Common carp of Caspian Sea is one of the economic fish, that have some morphenological differences, resistance in salt water and better quality in meet with domestic once and for using this fish in culture system, it is necessary to establish genetically parameters especially the heritability for selection purpose. The present study have been started from 2007 and lasted 3 years. The breeders were collected from the Gomishan station in south-east of Caspian Sea by fisherman and stocked in S.Rajaei fish restocking center and propagate based on the routine propagation technique in this farm. For heritability estimation a factorial crosses by 7×7 fish were done. Therefore, the fingerlings of 49 crosses were achieved, that kept first separately fiberglass tank and then in pond separately. Weight and length of fish were choice for heritability estimation. The average weight of juvenile common carp in 3 sampling stage of 90, 180 and 360 days, were 5.9, 35.7 and 84.7 gram and length of 9.6, 14.7 and 19.2 cm respectively. The heritability estimation for weight was 0.26 and length 0.25. Selection response based on selection difference by collection the more average breeders among their progeny were 36.4 g that means by selection every generation, there is about 4 percent improving in fish weight.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Morphological ; Common carp ; Cyprinus carpio ; Heritability ; Selection Response ; Culture ; Weight ; Length ; Fish ; Sampling ; Generation
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 46pp.
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  • 4
    Publication Date: 2021-05-19
    Description: Shrimp culture is one of the important fields in aquaculture which supply a part of necessary animal protein needed by the human being. Due to the limited catch of shrimp from the sea, its culture increasingly was considered. Shrimp culture industry was spreading quickly in developed and developing countries. The aim of this study was determine the bionormative parameters of vannamei shrimp culture using Caspian Sea brakish water in Mazandaran province, comparing the growth parameters of vannamei (Litopenaeus vannamei) shrimp culture with Caspian Sea brackish water and saline water of Persian Gulf and also possibility of introduction a new aquatic spciese for rereaing in Mazandaran province. The Shrimp Litopenaeus vannamei reared with the brackish water (10±0.43ppt) of the Caspian Sea in Mazandaran province and the Caspian Sea Ecological Research Iinstitute in summer 1391. In this study, post larvae-12 was prepared from Boshehr province. Initially, post larvae in brackish water were gradually adopted. The density of 35 per square pieces in an earthen pond (1000m2) was stocking.The rearing time was 85 days. Average daily growths: 0.31±0.037g; final mean weight: 21.11±0.71; Feed conversion ratio: 0.79 and final yield: 531kg were determined. In addition the experiment of 2012, the results of Mazandaran province vannamei shrimp culture, were observed in 2014 and 2015, the average weight average daily growing and FCR % were 20-18 g and 0.68 - 0.77 respectively during 90 days culture period with harvesting of 40 PL /m2 at cement culture pond. In 2014, average weight, average daily growing and FCR % were 22.6 g and 0.7, respectively during 60 days culture period with harvesting of 18 PL /m2 at earthen pond in Mazandaran province. Then the results of this study, wwere compared with scientific- research reports in field of vannamei shrimp culture from institute and fisheries research centers of south (Bushehr province) and north (Golestan province) of Iran. Important shrimp culture parameters were used in this comparing specially, growth parameters (average weight, average daily growing and survival rate), FCR, final crop, production per hectare and the best harvesting. Therefore, based on initial hypothesis and acquired results, was observred that, vannamie shrimp was reared well using Caspian Sea brakish water in Mazandaran province. In addition, it seems that, vannamei shrimp was gained better results in Mazandaran province compared to two other provinces, because of suitable climate conditions specially water salinity.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Vannamei shrimp ; Rereaing ; Saline water ; Brackish water ; Litopenaeus vannamei ; Shrimp culture
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 54pp.
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  • 5
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25304 | 18721 | 2018-09-08 16:49:09 | 25304 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The Common carp is the most important culture fish in the word, that can be consider as domestic fish. The Common carp of Caspian Sea is one of the economic fish, that have some morphenological differences, resistance in salt water and better quality in meet with domestic once and for using this fish in culture system, it is necessary to establish genetically parameters especially the heritability for selection purpose. The present study have been started from 2007 and lasted 3 years. The breeders were collected from the Gomishan station in south-east of Caspian Sea by fisherman and stocked in S.Rajaei fish restocking center and propagate based on the routine propagation technique in this farm. For heritability estimation a factorial crosses by 7×7 fish were done. Therefore, the fingerlings of 49 crosses were achieved, that kept first separately fiberglass tank and then in pond separately. Weight and length of fish were choice for heritability estimation. The average weight of juvenile common carp in 3 sampling stage of 90, 180 and 360 days, were 5.9, 35.7 and 84.7 gram and length of 9.6, 14.7 and 19.2 cm respectively. The heritability estimation for weight was 0.26 and length 0.25. Selection response based on selection difference by collection the more average breeders among their progeny were 36.4 g that means by selection every generation, there is about 4 percent improving in fish weight.
    Keywords: Aquaculture ; Iran ; Caspian Sea ; Gomishan ; Common carp ; Cyprinus carpio ; Heritability ; Selection Response ; Culture ; Weight ; Length ; Fish ; Sampling ; Generation
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 46
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  • 6
    facet.materialart.
    Unknown
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25639 | 18721 | 2018-10-08 05:54:55 | 25639 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: In this study lusiferse gene extracted from Vibrio fischeri. V. fischeri strain was kindly provided by Iranian Research Organization for Science and Technology (IROST). Genomic DNA extraction was carried out by Phenol-chloroform. A DNA fragment encoding the luxA and LuxB was amplified by PCR using sense and antisense primers, specific restriction sites for BamH1 and Kpn1 were introduced into 5´ end of forward and reverse primer, respectively. The PCR product was purified from agarose gel and ligated into cleaved PT257R cloning vector. Following the confirmation of the cloned Luciferase transformed into E. coli. Recombinant clones were confirmed by specific PCR and restriction enzyme digestion analysis. The luxA and LuxB fragment was released subcloned in to the PcDNA3.1\hug and PcDNA3.1\neo expression vectors, respectively. recombinant plasmid was confirmed through restriction digestion using BamH1 and Kpn1 enzymes and subsequently, transformation procedure continued into NIH3T3 eukaryote cells by specific kit. Luminescence ability of recombinant clones was tested by NIH3T3 cells and dechanal (substrate) and Neomysin and hygromysin. The results showed that luminescence start after 2 hours and then increase after 6 hours. Inaddition, the protein identity was verified by western blot analysis, the protein bands 76 kD were detected which indicates protein expression of luxB, luxA.
    Keywords: Biology ; Iran ; Lusifrase ; Vibrio fischeri ; Transfer gene ; DNA ; Lusiferase gene ; Investihation ; Genetic ; Cyprins carpio ; Rutilus frisii kutum
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 58
    Location Call Number Expected Availability
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  • 7
    Publication Date: 2021-07-16
    Description: Shrimp culture is one of the important fields in aquaculture which supply a part of necessary animal protein needed by the human being. Due to the limited catch of shrimp from the sea, its culture increasingly was considered. Shrimp culture industry was spreading quickly in developed and developing countries. The aim of this study was determine the bio normative parameters of vannamei shrimp culture using Caspian Sea brackish water in Mazandaran province, comparing the growth parameters of vannamei (Litopenaeus vannamei) shrimp culture with Caspian Sea brackish water and saline water of Persian Gulf and also possibility of introduction a new aquatic species for rereading in Mazandaran province. The Shrimp Litopenaeus vannamei reared with the brackish water (10±0.43ppt) of the Caspian Sea in Mazandaran province and the Caspian Sea Ecological Research Institute in summer 1391. In this study, post larvae-12 was prepared from Boshehr province. Initially, post larvae in brackish water were gradually adopted. The density of 35 per square pieces in an earthen pond (1000m2) was stocking. The rearing time was 85 days. Average daily growths: 0.31±0.037g; final mean weight: 21.11±0.71; Feed conversion ratio: 0.79 and final yield: 531kg were determined. In addition the experiment of 2012, the results of Mazandaran province vannamei shrimp culture, were observed in 2014 and 2015, the average weight average daily growing and FCR % were 20-18 g and 0.68 - 0.77 respectively during 90 days culture period with harvesting of 40 PL /m2 at cement culture pond. In 2014, average weight, average daily growing and FCR % were 22.6 g and 0.7, respectively during 60 days culture period with harvesting of 18 PL /m2 at earthen pond in Mazandaran province. Then the results of this study, were compared with scientific- research reports in field of vannamei shrimp culture from institute and fisheries research centers of south (Bushehr province) and north (Golestan province) of Iran. Important shrimp culture parameters were used in this comparing specially, growth parameters (average weight, average daily growing and survival rate), FCR, final crop, production per hectare and the best harvesting. Therefore, based on initial hypothesis and acquired results, was observed that, vannamei shrimp was reared well using Caspian Sea brackish water in Mazandaran province. In addition, it seems that, vannamei shrimp was gained better results in Mazandaran province compared to two other provinces, because of suitable climate conditions specially water salinity.
    Keywords: Aquaculture ; Iran ; Persian Gulf ; Caspian Sea ; Mazandaran province ; Vannamei shrimp ; Rereaing ; Saline water ; Brackish water ; Litopenaeus vannamei ; Shrimp culture
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 54
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