Publication Date:
2012-10-19
Description:
Barley (Hordeum vulgare L.) is among the world's earliest domesticated and most important crop plants. It is diploid with a large haploid genome of 5.1 gigabases (Gb). Here we present an integrated and ordered physical, genetic and functional sequence resource that describes the barley gene-space in a structured whole-genome context. We developed a physical map of 4.98 Gb, with more than 3.90 Gb anchored to a high-resolution genetic map. Projecting a deep whole-genome shotgun assembly, complementary DNA and deep RNA sequence data onto this framework supports 79,379 transcript clusters, including 26,159 'high-confidence' genes with homology support from other plant genomes. Abundant alternative splicing, premature termination codons and novel transcriptionally active regions suggest that post-transcriptional processing forms an important regulatory layer. Survey sequences from diverse accessions reveal a landscape of extensive single-nucleotide variation. Our data provide a platform for both genome-assisted research and enabling contemporary crop improvement.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉International Barley Genome Sequencing Consortium -- Mayer, Klaus F X -- Waugh, Robbie -- Brown, John W S -- Schulman, Alan -- Langridge, Peter -- Platzer, Matthias -- Fincher, Geoffrey B -- Muehlbauer, Gary J -- Sato, Kazuhiro -- Close, Timothy J -- Wise, Roger P -- Stein, Nils -- BB/100663X/1/Biotechnology and Biological Sciences Research Council/United Kingdom -- England -- Nature. 2012 Nov 29;491(7426):711-6. doi: 10.1038/nature11543. Epub 2012 Oct 17.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/23075845" target="_blank"〉PubMed〈/a〉
Keywords:
Alternative Splicing/genetics
;
Codon, Nonsense/genetics
;
Crops, Agricultural/genetics
;
Evolution, Molecular
;
Gene Expression Regulation, Plant
;
Genes, Plant/genetics
;
Genome, Plant/*genetics
;
Genomics
;
Hordeum/classification/*genetics
;
Molecular Sequence Annotation
;
Physical Chromosome Mapping
;
Polymorphism, Single Nucleotide/genetics
;
Repetitive Sequences, Nucleic Acid/genetics
;
*Sequence Analysis, DNA
;
Transcriptome/genetics
Print ISSN:
0028-0836
Electronic ISSN:
1476-4687
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
,
Natural Sciences in General
,
Physics
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