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  • 1
    Electronic Resource
    Electronic Resource
    Major flowering time gene and polygene effects on chickpea seed weight (2003)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 122 (2003), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The effect of the major flowering gene (PPD) on seed weight of chickpea was studied on 450 F3 families from reciprocal crosses between a small-seeded, early-flowering (PPD/PPD) type and a large-seeded, late flowering (PPD/PPD) cultivar. F4 progeny tests were carried out to determine the PPD genotypes of each individual F3. The effects of the PPD gene and the polygenes on mean seed weight were both significant. Genetic correlations between time to flowering and seed weight were positive and relatively high, suggesting that in certain genetic backgrounds it might be difficult to breed early-flowering cultivars without compromising seed weight.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.2003.00895.x
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  • 2
    Electronic Resource
    Electronic Resource
    Genetic control of seed weight and calcium concentration in chickpea seed (2000)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 119 (2000), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Chickpea, Cicer arietinum L., is a staple protein source in many Asian and Middle Eastern countries. Hence, the mineral content of its seed, especially that of calcium, is of nutritional importance. Calcium is transported through plants and to legume pods almost exclusively via the xylem stream, with Ca accretion in developing seeds resulting primarily from diffusion of Ca from the adjoining pod wall. Thus, for seeds of differing surface-to-mass ratios, Ca concentration is expected to correlate inversely with seed weight. The relationship between seed weight and Ca concentration in chickpea seeds was studied using a range of germplasm and derivatives from crosses between types differing in seed Ca concentration. Among the cultivars tested, low seed mass was associated with high Ca concentration. However, the study of hybrid progeny indicated that seed Ca content was mainly determined by genetic factors other than grain weight genes. This finding may assist in future breeding of high nutritional quality chickpea cultivars.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1439-0523.2000.00527.x
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  • 3
    Electronic Resource
    Electronic Resource
    Genetic diversity for drought resistance in wild emmer wheat and its ecogeographical associations (2005)
    Oxford, UK : Blackwell Science Ltd
    Plant, cell & environment 28 (2005), S. 0 
    Details
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Wild emmer wheat (Triticum turgidum spp. dicoccoides (Körn.) Thell.), the tetraploid progenitor of cultivated wheat, is a potential source for various agronomical traits, including drought resistance. The objectives of this study were to characterize (1) the genetic diversity for drought resistance in wild emmer wheat, and (2) the relationship between drought responses of the wild emmer germplasm and the ecogeographical parameters of its collection sites. A total of 110 wild emmer accessions consisting of 25 populations and three control durum wheat cultivars were examined under two irrigation regimes, well-watered (’wet’) and water-limited (’dry’). Wide genetic diversity was found both between and within the wild emmer populations in most variables under each treatment. A considerable number of the wild emmer accessions exhibited an advantage in productivity (spike and total dry matter) over their cultivated counterparts. Most wild emmer wheat accessions exhibited a greater carbon isotope ratio (δ13C, indicating higher water-use efficiency) under the dry treatment and higher plasticity of δ13C relative to the cultivated controls, which may have contributed to the drought adaptations in the former. The most outstanding drought-tolerance capacity (in term of productivity under the dry treatment and susceptibility indices) was detected in wild emmer populations originated from hot dry locations. The results suggest that wild emmer has the potential to improve drought resistance in cultivated wheat.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3040.2005.01259.x
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  • 4
    Electronic Resource
    Electronic Resource
    Key Features of Cereal Genome Organization as Revealed by the Use of Cytosine Methylation-Sensitive Restriction Endonucleases
    Amsterdam : Elsevier
    Genomics 15 (1993), S. 472-482 
    Details
    ISSN: 0888-7543
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1006/geno.1993.1097
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  • 5
    Electronic Resource
    Electronic Resource
    Modified CTAB Procedure for DNA Isolation from Epiphytic Cacti of the Genera Hylocereus and Selenicereus (Cactaceae) (1999)
    Springer
    Plant molecular biology reporter 17 (1999), S. 249-254 
    Details
    ISSN: 1572-9818
    Keywords: cacti ; DNA extraction ; Hylocereus ; polysaccharides ; Selenicereus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We present a simple protocol for DNA isolation from climbing cacti, genera Hylocereus and Selenicereus. The abundant polysaccharides present in Hylocereus and Selenicereus species interfere with DNA isolation, and DNA extracts, rich in polysaccharides, are poor templates for amplification using polymerase chain reaction (PCR). We used roots as the source tissue due to the lower viscosity of the extracts relative to that of other tissues. The extraction and isolation procedure we devised consists of the following steps: (1) three washes of ground tissue with the extraction buffer to remove the polysaccharides; (2) extraction with high-salt (4 M NaCl) cetyltrimethylammonium bromide (CTAB) buffer to remove the remaining polysaccharides; (3) removal of RNA by RNase; (4) phenol:chloroform extraction to remove proteins; (5) chloroform extraction to remove remaining phenols. The yields ranged from 10 to 20 μg DNA/g fresh roots. DNA samples prepared by our method were consistently amplifiable in the RAPD reaction and gave reproducible profiles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1007656315275
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  • 6
    Electronic Resource
    Electronic Resource
    Identification of a chromosome-specific probe that maps within the Ph1 deletions in common and durum wheat (1997)
    Springer
    Theoretical and applied genetics 94 (1997), S. 968-970 
    Details
    ISSN: 1432-2242
    Keywords: Key words Chromosome-specific DNA sequence ; Ph1 gene ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The Ph1 (pairing homoeologous) gene is the major factor that determines the diploid-like chromosome behavior of polyploid wheat. This gene, which is located on the long arm of chromosome 5B (5BL), suppresses homoeologous pairing at meiosis while allowing exclusive homologous pairing. In an effort to tag the specific chromosomal region where this gene is located, we have previously microdissected chromosome arm 5BL from bread wheat and produced a plasmid library by random PCR amplification and cloning. In this work we isolated from this library a 5BL-specific probe, WPG90, and mapped it within the interstitial deleted chromosome fragments carrying Ph1 in common and durum wheat. A PCR assay of Ph1 based on WPG90 was developed that allows an easy identification of homozygous genotypes deficient for this gene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050502
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  • 7
    Electronic Resource
    Electronic Resource
    Characterization of the calmodulin gene family in wheat: structure, chromosomal location, and evolutionary aspects (1996)
    Springer
    Molecular genetics and genomics 252 (1996), S. 684-694 
    Details
    ISSN: 1617-4623
    Keywords: Key words Aneuploid ; Calcium ; Root ; Triticum ; Wild wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Calmodulin is a ubiquitous transducer of calcium signals in eukaryotes. In diploid plant species, several isoforms of calmodulin have been described. Here, we report on the isolation and characterization of calmodulin cDNAs corresponding to 10 genes from hexaploid (bread) wheat (Triticum aestivum). These genes encode three distinct calmodulin isoforms; one isoform is novel in that it lacks a conserved calcium binding site. Based on their nucleotide sequences, the 10 cDNAs were classified into four subfamilies. Using subfamily-specific DNA probes, calmodulin genes were identified and the chromosomal location of each subfamily was determined by Southern analysis of selected aneuploid lines. The data suggest that hexaploid wheat possesses at least 13 calmodulin-related genes. Subfamilies 1 and 2 were both localized to the short arms of homoeologous-group 3 chromosomes; subfamily 2 is located on all three homoeologous short arms (3AS, 3BS and 3DS), whereas subfamily 1 is located only on 3AS and 3BS but not on 3DS. Further analysis revealed that Aegilops tauschii, the presumed diploid donor of the D-genome of hexaploid wheat, lacks a subfamily-1 calmodulin gene homologue, whereas diploid species related to the progenitors of the A and B genomes do contain such genes. Subfamily 3 was localized to the short arm of homoeologous chromosomes 2A, 2B and 2D, and subfamily 4 was mapped to the proximal regions of 4AS, 4BL and 4DL. These findings suggest that the calmodulin genes within each subfamily in hexaploid wheat represent homoeoallelic loci. Furthermore, they also suggest that calmodulin genes diversified into subfamilies before speciation of Triticum and Aegilops diploid species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02173974
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  • 8
    Electronic Resource
    Electronic Resource
    A family of related sequences associated with (TTTAGGG)n repeats are located in the interstitial regions of wheat chromosomes (1994)
    Springer
    Molecular genetics and genomics 245 (1994), S. 349-354 
    Details
    ISSN: 1617-4623
    Keywords: Wheat ; TTTAGGG-associated Polymorphic sequences ; Interstitial repeats RFLP map
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A family of related sequences associated with (TTTAGGG) n repeats has been cloned from the wheat cultivar Chinese Spring. These sequences reveal a high level of polymorphism between wheat varieties when used as restriction fragment length polymorphism (RFLP) probes. Although this family of sequences contains motifs homologous to the repeats in the telomeres of wheat, they are located at interstitial sites on wheat chromosomes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00290115
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  • 9
    Electronic Resource
    Electronic Resource
    Characterization of the calmodulin gene family in wheat: structure, chromosomal location, and evolutionary aspects (1996)
    Springer
    Molecular genetics and genomics 252 (1996), S. 684-694 
    Details
    ISSN: 1617-4623
    Keywords: Aneuploid ; Calcium ; Root ; Triticum ; Wild wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Calmodulin is a ubiquitous transducer of calcium signals in eukaryotes. In diploid plant species, several isoforms of calmodulin have been described. Here, we report on the isolation and characterization of calmodulin cDNAs corresponding to 10 genes from hexaploid (bread) wheat (Triticum aestivum). These genes encode three distinct calmodulin isoforms; one isoform is novel in that it lacks a conserved calcium binding site. Based on their nucleotide sequences, the 10 cDNAs were classified into four subfamilies. Using subfamily-specific DNA probes, calmodulin genes were identified and the chromosomal location of each subfamily was determined by Southern analysis of selected aneuploid lines. The data suggest that hexaploid wheat possesses at least 13 calmodulin-related genes. Subfamilies 1 and 2 were both localized to the short arms of homoeologous-group 3 chromosomes; subfamily 2 is located on all three homoeologous short arms (3AS, 3BS and 3DS), whereas subfamily 1 is located only on 3AS and 3BS but not on 3DS. Further analysis revealed thatAegilops tauschii, the presumed diploid donor of the D-genome of hexaploid wheat, lacks a subfamily-1 calmodulin gene homologue, whereas diploid species related to the progenitors of the A and B genomes do contain such genes. Subfamily 3 was localized to the short arm of homoeologous chromosomes 2A, 2B and 2D, and subfamily 4 was mapped to the proximal regions of 4AS, 4BL and 4DL. These findings suggest that the calmodulin genes within each subfamily in hexaploid wheat represent homoeoallelic loci. Furthermore, they also suggest that calmodulin genes diversified into subfamilies before speciation ofTriticum andAegilops diploid species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02173974
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  • 10
    Electronic Resource
    Electronic Resource
    Genetic characterization of Lens ervoides from Ethiopia (1992)
    Springer
    Genetic resources and crop evolution 39 (1992), S. 51-54 
    Details
    ISSN: 1573-5109
    Keywords: dwarfing genes ; isozyme profile ; wild lentils ; genetic resource
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A single population of the Mediterranean species Lens ervoides was located in Ethiopia and seeds were collected. Based on chromosomal arrangement and isozyme profile the Ethiopian L. ervoides is similar to populations of this species in the Mediterranean region. All hybrids between L. ervoides from Ethiopia and an accession of this species from Israel were dwarf, whereas hybrids between the former and an accession from Algeria were normal. Segregation in F2 and BC generations indicated that the dwarf habit in lentil is governed by dominant alleles of two complementary genes designated df 1 and df 2. The dominant allele of another gene, dfi, inhibits the dwarf phenotype. The evolution of L. ervoides from Ethiopia and its potential as a genetic resource are briefly discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00052653
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