ISSN:
1013-9826
Source:
Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
Topics:
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
Initial attachment and spreading of the inoculated cells determines the long-time viabilityof cells onto biomedical scaffolds designed for various orthopedic or other clinical applications. Theaim of this study was to investigate the influence of biomimetic thin film coating surfaces ofbio-derived bone scaffolds with collagen proteins and chitosan on bone marrow mesenchymal stemcells interactions in order to improve cell adhesion, spreading and proliferation. These two meritswere used synthetically to generate apatite-based materials that can function as allograft bone graftsin humans. In this study, the thin film coatings were operated by means of soaked, pre-frozen, andfreeze-dried step by step. All coatings were characterized using Raman spectra, inverted microscope,atomic force microscopy, and scanning electron microscopy. After that, the bio-derived bonescaffolds with or without thin film coatings were used in bone marrow mesenchymal stem cell cultureexperiments to study cell adhesion, spreading, viability, proliferation and morphology. Then, thebiological morphologies of the fabricated cell-scaffold constructs were detected by scanning electronmicroscope (SEM). The cell reactions were investigated concerning cell adhesion, migration,spreading, and proliferation under inverted microscope and fluorescence microscopy. The resultsshowed that the bio-derived bone scaffold treated with thin film coatings by using rat-tail type Icollagen and chitosan improved the adhesion and spreading of mesenchymal stem cells incomparison to the untreated one. Besides, cell viability and morphology were not affected by thepresence of either type of thin film coating. Still, the results assay revealed an increased proliferationof bone marrow mesenchymal stem cells on both types of thin film coatings compared to coating withnon-coated controls
Type of Medium:
Electronic Resource
URL:
http://www.tib-hannover.de/fulltexts/2011/0528/01/57/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.373-374.722.pdf
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