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  • 1
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    Adaptation of Spodoptera exigua larvae to plant proteinase inhibitors by induction of gut proteinase activity insensitive to inhibition. (1995)
    National Academy of Sciences
    Details
    Publication Date: 1995-08-15
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
    Published by National Academy of Sciences
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    PAPER CURRENT
  • 2
    Electronic Resource
    Electronic Resource
    Thionins: properties, possible biological roles and mechanisms of action (1994)
    Springer
    Plant molecular biology 26 (1994), S. 25-37 
    Details
    ISSN: 1573-5028
    Keywords: Antimicrobial ; ion channel ; phosphoinositides ; plant defence ; thionins ; toxins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Thionins are low-molecular-weight proteins (M r ca. 5000) occurring in seeds, stems, roots and leaves of a number of plant species. The different members of this family of plant proteins show both sequence and structural homology, and are toxic to bacteria, fungi, yeasts and various naked cells in vitro. Toxicity requires an electrostatic interaction of the positively charged thionin with the negatively charged phospholipids making up the membrane, followed by either pore formation or a specific interaction with a certain lipid domain. This domain might be composed of phosphoinositides, which mediate transduction of environmental signals in eukaryotes. Their in vitro toxicity to plant pathogenic bacteria and fungi could reflect a direct role in plant defence, although, in view of the many divergent activities displayed by thionins both in vitro and in vivo, a biological role other than inhibition of microbial growth is equally plausible.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00039517
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  • 3
    Electronic Resource
    Electronic Resource
    Fate of introduced genetic markers in transformed root clones and regenerated plants of monohaploid and diploid potato genotypes (1989)
    Springer
    Theoretical and applied genetics 78 (1989), S. 185-193 
    Details
    ISSN: 1432-2242
    Keywords: Potato ; Agrobacterium transformation ; Plant regeneration ; Monohaploid, diploid ; Genetic markers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Agrobacterium transformation of stem internodes of four monohaploid (839-79, 849-7, 851-23, 855-1) and two diploid (M9 and HH260) potato genotypes using hairy root-inducing single (LBA 1020, LBA 9365, LBA 9402) and binary (LBA 1060KG) vectors is reported. Various media and successive culture steps were tested for plant regeneration from different transformed root clones. The fate of introduced genetic markers in root clones and regenerated plants (hairy root phenotype, hormone autotrophy, opine production, kanamycin resistance, β-glucuronidase activity), the ploidy stability and protoplast yield were analysed. The transformation efficiency of stem internodes (hairy root production) and the regeneration capacity of the transformed root clones greatly differed within and between the various potato genotypes. The regenerated plants obtained after transformation with both types of vectors often showed the absence of one or more genetic markers. However, transformation with the binary Agrobacterium vector generally resulted in the stable presence of the opines in all transformed root clones and most regenerated plants. In HH260, transformation efficiency, plant regeneration of transformed root clones, protoplast yield and ploidy stability were the highest as compared to the other genotypes. The application of these transformed plants as marker lines in gene mapping and gene expression studies is indicated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00288798
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  • 4
    Electronic Resource
    Electronic Resource
    Isolation and characterization of RAPD-based markers linked to the beet cyst nematode resistance locus (Hs1 pat-1) on chromosome 1 of B. patellaris (1995)
    Springer
    Theoretical and applied genetics 90 (1995), S. 885-891 
    Details
    ISSN: 1432-2242
    Keywords: Randomly amplified polymorphic DNA (RAPD) ; Deletion mapping ; Sequence Tagged Site (STS) ; Monosomic fragment additions ; Beet cyst nematode resistance ; Heterodera schachtii Schm ; Beta patellaris
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A beet cyst nematode (BCN)-resistant telosomic addition of B. patellaris chromosome 1 in B. vulgaris was used to isolate 6 RAPD markers linked to the BCN resistance locus Hs1 pat-1. Southern analysis showed that the analyzed RAPD products contain either low-, middle or high-repetitive DNA. The relative positions of the random amplified polymorphic DNA (RAPD) markers and of the restriction fragment length polymorphism (RFLP) loci corresponding to the low-repetitive RAPD products were determined by deletion mapping using a panel of seven nematode-resistant B. patellaris chromosome-1 fragment additions. One RAPD marker, OPB11800, was found to be present in two copies on the long arm telosome of B. patellaris chromosome 1. These copies are closely linked to the BCN resistance gene and flank the gene on both sides. On the basis of the nucleotide sequence of OPB11800, sequence-tagged site (STS) primers were developed that amplify specific fragments derived from the two OPB11800 loci. These STS markers can be used in the map-based cloning of the BCN gene, as they define start and finishing points of a chromosomal walk towards the Hs1 pat-1 locus. Two copies of the middle-repetitive OPX21100 marker were mapped in the same interval of the deletion mapping panel as the resistance gene locus and thereby belong to the nearest markers as yet found for the BCN gene in B. patellaris.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222027
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  • 5
    Electronic Resource
    Electronic Resource
    A genetic map of potato (Solanum tuberosum) integrating molecular markers, including transposons, and classical markers (1995)
    Springer
    Theoretical and applied genetics 91 (1995), S. 289-300 
    Details
    ISSN: 1432-2242
    Keywords: Morphological markers ; Isozymes ; Non-inbred species ; Combined map ; JoinMap
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A genetic map of potato (Solanum tuberosum L.) integrating molecular markers with morphological and isozyme markers was constructed using a backcross population of 67 diploid potato plants. A general method for map construction is described that differs from previous methods employed in potato and other outbreeding plants. First, separate maps for the female and male parents were constructed. The female map contained 132 markers, whereas the male map contained 138 markers. Second, on the basis of the markers in common the two integrated parental maps were combined into one with the computer programme JoinMap. This combined map consisted of 175 molecular markers, 10 morphological markers and 8 isozyme markers. Ninety-two of the molecular markers were derived from DNA sequences flanking either T-DNA inserts in potato or reintegrated maize transposable elements originating from these T-DNA constructs. Clusters of distorted segregation were found on chromosomes 1,2,8 and 11 for the male parent and chromosome 5 for both parents. The total length of the combined map is 1120 cM.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220891
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  • 6
    Electronic Resource
    Electronic Resource
    Mapping of QTLs involved in nematode resistance, tuber yield and root development in Solanum sp. (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 463-470 
    Details
    ISSN: 1432-2242
    Keywords: Key words  RFLPs ; Solanum spegazzinii ; Globodera sp. ; Interlocus interaction ; Intralocus interaction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract   A backcross population, derived from the cross (S. tuberosum×S. spegazzinii)×S. tuberosum was used to map QTLs involved in nematode resistance, tuber yield and root development. Complete linkage maps were available for the interspecific hybrid parent as well as the S. tuberosum parent, and interval mapping for all traits was performed for both. Additionally, the intra- and inter-locus interactions of the QTLs were examined. The Gro1.2 locus, involved in resistance to G. rostochiensis pathotype Ro1, that was previously mapped in the S. tuberosum×S. spegazzinii F1 population, was located more precisely on chromosome 10. A new resistance locus, Gro1.4, also conferring resistance to G. rostochiensis pathotype Ro1, was found on chromosome 3. Different alleles of this locus originating from both parents contributed to the resistant phenotype, indicating multiallelism at this locus. No interlocus interactions were observed between these two resistance loci. For resistance to G. pallida no QTLs were detected. One minor QTL involved in tuber yield was located on chromosome 4. Two QTLs involved in root development and having large effects were mapped on chromosomes 2 and 6 and an epistatic interaction was found between these two loci.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050150
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  • 7
    Electronic Resource
    Electronic Resource
    Mapping of loci involved in quantitatively inherited resistance to the potato cyst-nematode Globodera rostochiensis pathotype Ro1 (1993)
    Springer
    Theoretical and applied genetics 87 (1993), S. 464-470 
    Details
    ISSN: 1432-2242
    Keywords: Solanum spegazzinii ; Globodera rostochi ensis ; Nematode resistance ; QTL ; RFLP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We report the identification and mapping of two quantitative trait loci (QTLs) of Solanum spegazzinii BGRC, accession 8218-15, involved in resistance to the potato cyst-nematode Globodera rostochiensis pathotype Ro1, by means of restriction fragment length polymorphisms (RFLPs). For this purpose we crossed a susceptible diploid S. tuberosum with the resistant S. spegazzinii, and tested the F1 population for resistance to the Ro1 pathotype. Since the F1 segregated for the resistance, the S. spegazzinii parent was concluded to be heterozygous at the nematode resistance loci. For the mapping of the resistance loci we made use of RFLP markers segregating for S. spegazzinii alleles in the F1. One hundred and seven RFLP markers were tested in combination with four different restriction enzymes; 29 of these displayed a heterozygous RFLP pattern within S. spegazzinii and were used for mapping. Analysis of variance (ANOVA) was applied to test the association of the RFLP patterns of these markers with nematode resistance. Two QTLs involved in disease resistance to Globodera rostochiensis pathotype Ro1 were identified and mapped to chromosomes 10 and 11 respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00215092
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  • 8
    Electronic Resource
    Electronic Resource
    Quantitatively-inherited resistance toGlobodera pallida is dominated by one major locus inSolanum spegazzinii (1994)
    Springer
    Theoretical and applied genetics 88 (1994), S. 764-769 
    Details
    ISSN: 1432-2242
    Keywords: Potato cyst-nematode ; Quantitative trait loci RFLP ; Distorted segregation ; Solanum spegazzinii
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A high level of resistance toGlobodera pallida pathotypes Pa2 and Pa3 exists inSolanum spegazzinii, a wild relative of potato (S. tuberosum ssp.tuberosum). Here we report the mapping of loci involved in quantitatively-inherited nematode resistance with the use of RFLPs. One major locus,Gpa, was mapped on chromosome 5 and two minor loci on chromosomes 4 and 7 ofS. spegazzinii. Additionally, the contribution of the susceptible parent to nematode resistance was determined. TheGpa locus was solely responsible for the high resistance level found in the segregating population. However, the RFLP marker closely linked to this resistance locus showed a distorted segregation, with a shortage of plants having the resistance linked allele. Our results indicate that a prediction of the genetic constitution of a quantitative trait based solely on phenotypic observations can lead to erroneous conclusions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01253983
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  • 9
    Electronic Resource
    Electronic Resource
    Mapping of QTLs involved in nematode resistance, tuber yield and root development in Solanum sp. (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 463-470 
    Details
    ISSN: 1432-2242
    Keywords: RFLPs ; Solanum spegazzinii ; Globodera sp ; Interlocus interaction ; Intralocus interaction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A backcross population, derived from the cross (S. tuberosumxS. spegazzinii)xS. tuberosum was used to map QTLs involved in nematode resistance, tuber yield and root development. Complete linkage maps were available for the interspecific hybrid parent as well as the S. tuberosum parent, and interval mapping for all traits was performed for both. Additionally, the intra- and inter-locus interactions of the QTLs were examined. The Gro1.2 locus, involved in resistance to G. rostochiensis pathotype Ro1, that was previously mapped in the S. tuberosumxS. spegazzinii F1 population, was located more precisely on chromosome 10. A new resistance locus, Gro1.4, also conferring resistance to G. rostochiensis pathotype Ro1, was found on chromosome 3. Different alleles of this locus originating from both parents contributed to the resistant phenotype, indicating multiallelism at this locus. No interlocus interactions were observed between these two resistance loci. For resistance to G. pallida no QTLs were detected. One minor QTL involved in tuber yield was located on chromosome 4. Two QTLs involved in root development and having large effects were mapped on chromosomes 2 and 6 and an epistatic interaction was found between these two loci.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223694
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  • 10
    Electronic Resource
    Electronic Resource
    The impact on biosafety of the phosphinothricin-tolerance transgene in inter-specific B. rapa×B. napus hybrids and their successive backcrosses (1997)
    Springer
    Theoretical and applied genetics 95 (1997), S. 442-450 
    Details
    ISSN: 1432-2242
    Keywords: Key words Biosafety ; Brassica rapa ; Inter-specific hybridization ; Phosphinothricin-tolerance gene ; Transgenic Brassica napus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  There is strong evidence indicating that gene flow from transgenic B. napus into weedy wild relatives is inevitable following commercial release. Research should now focus on the transmission, stability, and impact of transgene expression after the initial hybridization event. The present study investigated the transfer of a phosphinothricin-tolerance transgene by inter-specific hybridization between B. rapa and two transgenic B. napus lines. The expression of the transgene was monitored in the F1 hybrids and in subsequent backcross generations. The transgene was transmitted relatively easily into the F1 hybrids and retained activity. Large differences in the transmission frequency of the transgene were noted between offspring of the two transgenic lines during backcrossing. The most plausible explanation of these results is that the line showing least transmission during backcrossing contains a transgene integrated into a C-genome chromosome. Approximately 10% of offspring retained the tolerant trait in the BC3 and BC4 generations. The implications of these findings for the stable introgression of transgenes carried on one of the chromosomes of the C-genome from B. napus and into B. rapa are briefly discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050581
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