Abstract
A backcross population, derived from the cross (S. tuberosumxS. spegazzinii)xS. tuberosum was used to map QTLs involved in nematode resistance, tuber yield and root development. Complete linkage maps were available for the interspecific hybrid parent as well as the S. tuberosum parent, and interval mapping for all traits was performed for both. Additionally, the intra- and inter-locus interactions of the QTLs were examined. The Gro1.2 locus, involved in resistance to G. rostochiensis pathotype Ro1, that was previously mapped in the S. tuberosumxS. spegazzinii F1 population, was located more precisely on chromosome 10. A new resistance locus, Gro1.4, also conferring resistance to G. rostochiensis pathotype Ro1, was found on chromosome 3. Different alleles of this locus originating from both parents contributed to the resistant phenotype, indicating multiallelism at this locus. No interlocus interactions were observed between these two resistance loci. For resistance to G. pallida no QTLs were detected. One minor QTL involved in tuber yield was located on chromosome 4. Two QTLs involved in root development and having large effects were mapped on chromosomes 2 and 6 and an epistatic interaction was found between these two loci.
Similar content being viewed by others
References
Arntzen FK, Van Eeuwijk FA (1992) Variation in the resistance level of the potato genotype and the virulence level of potato cystnematode populations. Euphytica 62:135–143
Barone A, Ritter E, Schachtschabel U, Debener T, Salamini F, Gebhardt C (1990) Localization by restriction fragment length polymorphism mapping in potato of a major dominant gene conferring resistance to the potato cyst-nematode Globodera rostochiensis. Mol Gen Genet 224:177–182
Botstein D, White RL, Skolnick M, Davis RW (1980) Construction of a genetic map in man using restriction fragment length polymorphisms. Am J Hum Genet 32:314–331
De Vicente MC, Tanksley SD (1993) QTL analysis of transgressive segregation in an interspecific tomato cross. Genetics 134:585–596
East EM (1915) Studies on size inheritance in Nicotiana. Genetics 1:164–176
Edwards MD, Stuber CW, Wendel JF (1987) Molecular-marker-facilitated investigations of quantitative trait loci in maize. 1. Numbers, genomic distribution, and types of gene action. Genetics 116:113–125.
Fatokun CA, Menancio-Hautea DI, Danesh D, Young ND (1992) Evidence for orthologous seed-weight genes in cowpea and mung bean based on RFLP mapping. Genetics 132:841–846
Gebhardt C, Mugniery D, Ritter E, Salamini F, Bonnel E (1993) Identification of RFLP markers closely linked to the Hl gene conferring resistance to Globodera rostochiensis in potato. Theor Appl Genet 85:541–544
Jacobs JME, Van Eck HJ, Arens PEP, Verkerk-Bakker B, Te Lintel Hekkert B et al. (1995) A genetic map of potato (Solanum tuberosum) integrating molecular markers, including transposons, and classical markers. Theor Appl Genet (in press)
Johanssen W (1909) Elemente der exakten Erblichkeitslehre. Fischer, Jena
Keim P, Diers BW, Olson TC, Schoemaker RC (1990) RFLP mapping in soybean: associations between marker loci and variation in quantitative traits. Genetics 126:735–742
Kreike CM, De Koning JRA, Vinke JH, Van Ooijen JW, Gebhardt C, Stiekema WJ (1993) Mapping of loci involved in quantitatively inherited resistance to the potato cyst-nematode Globodera rostochiensis pathotype Ro1. Theor Appl Genet 87:464–470
Kreike CM, De Koning JRA, Vinke JH, Van Ooijen JW, Stiekema WJ (1994) Quantitatively inherited resistance to Globodera pallida is dominated by one major locus in Solanum spegazzinii. Theor Appl Genet 88:764–769
Lander ES, Botstein D (1989) Mapping Mendelian factors underlying quantitative traits using RFLP linkage maps. Genetics 121:185–756
Nilsson-Ehle H (1919) Kreuzunguntersuchungen an Hafer und Weizen. Lunds Univ Aarskr NF5:1–122
Leonards-Schippers C, Gieffen W, Schäfer-Pregl R, Ritter E, Knapp SJ et al. (1994) Quantitative resistance to Phytophthora infestons in potato: a case study for QTL maping in an allogamous species. Genetics 137:67–77
Paterson AH, Lander ES, Hewitt JD, Peterson S, Lincoln SE, Tanksley SD (1988) Resolution of quantitative traits into Mendelian factors, using a complete linkage map of restriction fragment length polymorphisms. Nature 335:721–726
Paterson AH, Damon S, Hewitt JD, Zamir D, Rabinowitch HD, Lincoln SE, Lander ES, Tanksley SD (1991) Mendelian factors underlying quantitative traits in tomato: comparison across species, generations, and environments. Genetics 127:181–197
Pineda O, Bonierbale MW, Plaisted RL, Brodie BB, Tanksley SD (1993) Identification of RFLP markers linked to the H1 gene conferring resistance to the potato cyst nematode Globodera rostochiensis. Genome 36:152–156
Sax K (1923) The association of size differences with seed coat pattern and pigmentation in Phaseolus vulgaris. Genetics 8:552–560
Stam P (1993) Construction of integrated linkage maps by means of a new computer package: JoinMap. The Plant Journal 3:739–744
Stuber CW, Lincoln SE, Wolff DW, Helentjaris T, Lander ES (1992) Identification of genetic factors contributing to heterosis in a hybrid from two elite maize inbred lines using molecular markers. Genetics 132:823–839
Thoday JM (1961) Location of polygenes. Nature 191:368–370
Van Eck HJ, Jacobs JME, Stam P, Ton J, Stiekema WJ, Jacobsen E (1994) Multiple alleles for tuber shape in diploid potato detected by qualitative and quantitative genetic analysis using RFLPs. Genetics 137:303–309
Van Ooijen JW (1992) Accuracy of mapping quantitative trait loci in autogamous species. Theor Appl Genet 84:803–811
Author information
Authors and Affiliations
Additional information
Communicated by F. Salamini
Rights and permissions
About this article
Cite this article
Kreike, C.M., Kok-Westeneng, A.A., Vinke, J.H. et al. Mapping of QTLs involved in nematode resistance, tuber yield and root development in Solanum sp.. Theoret. Appl. Genetics 92, 463–470 (1996). https://doi.org/10.1007/BF00223694
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00223694