Skip to main content
SpringerLink
Log in

A genetic map of potato (Solanum tuberosum) integrating molecular markers, including transposons, and classical markers

  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

A genetic map of potato (Solanum tuberosum L.) integrating molecular markers with morphological and isozyme markers was constructed using a backcross population of 67 diploid potato plants. A general method for map construction is described that differs from previous methods employed in potato and other outbreeding plants. First, separate maps for the female and male parents were constructed. The female map contained 132 markers, whereas the male map contained 138 markers. Second, on the basis of the markers in common the two integrated parental maps were combined into one with the computer programme JoinMap. This combined map consisted of 175 molecular markers, 10 morphological markers and 8 isozyme markers. Ninety-two of the molecular markers were derived from DNA sequences flanking either T-DNA inserts in potato or reintegrated maize transposable elements originating from these T-DNA constructs. Clusters of distorted segregation were found on chromosomes 1,2,8 and 11 for the male parent and chromosome 5 for both parents. The total length of the combined map is 1120 cM.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Anonymous (1988) 63e Beschrijvende Rassenlijst voor Landbouwgewassen. Leiter-Nypels b.v., Maastricht

  • Bonierbale MW, Plaisted RL, Tanksley SD (1988) RFLP maps based on a common set of clones reveal modes of chromosomal evolution in potato and tomato. Genetics 120:1095–1103

    Google Scholar 

  • Bonierbale MW, Plaisted RL, Pineda O, Tanksley SD (1994) QTL analysis of trichome-mediated insect resistance in potato. Theor Appl Genet 87:973–987

    Google Scholar 

  • De Jong H (1983) Inheritance of sensitivity to the herbicide metribuzin in cultivated diploid potatoes. Euphytica 32:41–48

    Google Scholar 

  • De Jong H, Rowe PR (1972) Genetic markers in inbred clones of cultivated diploid potatoes. Potato Res 15:200–208

    Google Scholar 

  • Dodds KS, Long DH (1956) The inheritance of colour in diploid potatoes. II. A three-factor linkage group. J Genet 54:27–41

    Google Scholar 

  • Douches DS, Quiros CF (1987) Use of 4x-2x crosses to determine gene-centromere map distances of isozyme loci in Solanum species. Genome 29:519–527

    Google Scholar 

  • Douches DS, Quiros CF (1988a) Genetic recombination in a diploid synaptic mutant and a Solanum tuberosumxS. chacoense diploid hybrid. Heredity 60:183–191

    Google Scholar 

  • Douches DS, Quiros CF (1988b) Additional isozyme loci in tuberbearing Solanums: inheritance and linkage relationships. J Hered 79:377–384

    Google Scholar 

  • Dower WJ, Miller JF, Ragsdale CW (1988) High efficiency transformation of E. coli by high voltage electroporation. Nucleic Acids Res 16:6127–6145

    CAS  PubMed  Google Scholar 

  • Gebhardt C, Ritter E, Debener T, Schachtschabel U, Walkemeier B, Uhrig H, Salamini F (1989) RFLP analysis and linkage mapping in Solanum tuberosum. Theor Appl Genet 78:65–75

    Google Scholar 

  • Gebhardt C, Ritter E, Barone A, Debener T, Walkemeier B, Schachtschabel U, Kaufmann H, Thompson RD, Bonierbale MW, Ganal MW, Tanksley SD, Salamini F (1991) RFLP maps of potato and their alignment with the homologous tomato genome. Theor Appl Genet 83:49–57

    Google Scholar 

  • Gebhardt C, Ritter E, Salamini F (1994) RFLP map of the potato. In: Phillips RL, Vasil IK (eds) DNA-based markers in plants. Kluwer, Dordrecht/Boston, pp 271–285

    Google Scholar 

  • Giraudat J, Hauge BM, Valon C, Snalle J, Parcy F, Goodman HM (1992) Isolation of the Arabidopsis ABI3 gene by positional cloning. Plant Cell 4:1251–1261

    Article  CAS  PubMed  Google Scholar 

  • Hanneman RE, Peloquin SJ (1967) Crossability of 24-chromosome potato hybrids with 48-chromosome cultivars. Eur Potato J 10:62–73

    Google Scholar 

  • Hermsen JGTh, Verdenius J (1973) Selection from Solanum tuberosum Group phureja of genotypes combining high frequency haploid induction with homozygosity for embryo-spot. Euphytica 22:244–259

    Google Scholar 

  • Howard HW (1970) The genetics of the potato Solanum tuberosum. Logos Press, London

    Google Scholar 

  • Jacobs JME, Te Lintel Hekkert B, El-Kharbotly A, Jacobsen E, Stiekema WJ, Pereira A (1994) Ac-Ds transposons mapped near disease resistance loci for targeted tagging in potato. In: Belknap WR, Vayda ME, Park WD (eds) Molecular and cellular biology of the potato, 2nd edn. CAB Int, Wallingford, UK, pp 21–30

    Google Scholar 

  • Jacobsen E (1978) Die Chromosomen-Verdopplung in der Züchtung dihaploider Kartoffeln. PhD thesis, Rheinische Friedrich-Wilhelm-Universität, Bonn

    Google Scholar 

  • Jacobsen E (1980) Increase of diplandroid formation and seed set in 4x×2x crosses in potatoes by genetical manipulation of dihaploids and some theoretical consequences. Z Pflanzenzuecht 85:110–121

    Google Scholar 

  • Jongedijk E, Ramanna MS (1988) Synaptic mutants in potato, Solanum tuberosum L. I. Expression and identity of genes for desynapsis. Genome 30:664–670

    Google Scholar 

  • Jongedijk E, Ramanna MS (1989) Synaptic mutants in potato, Solanum tuberosum L. II. Concurrent reduction of chiasma frequencies in male and female meiosis of ds-1 (desynapsis) mutants. Genome 32:1054–1062

    Google Scholar 

  • Jongedijk E, VanderWolk JMASA, Suurs LCJM (1990) Analysis of glutamate oxaloacetate transaminase (GOT) isozyme variants in diploid tuberous Solanum; inheritance and linkage relationships to ds (desynapsis), y (tuber flesh colour), cr (crumpled) and yc (yellow cotyledon). Euphytica 45:155–167

    Google Scholar 

  • Knapp S, Coupland G, Uhrig H, Starlinger P, Salamini F (1988) Transposition of the maize transposable element Ac in Solanum tuberosum. Mol Gen Genet 213:285–290

    Google Scholar 

  • Kreike CM, De Koning JRA, Vinke JH, Van Ooijen JW, Gebhardt C, Stiekema WJ (1993) Mapping of loci involved in quantitatively inherited resistance to the potato cyst-nematode Globodera rostochiensis pathotype Ro1. Theor Appl Genet 87:464–470

    Google Scholar 

  • Leonards-Schippers C, Gieffers W, Schäfer-Pregl R, Ritter E, Knapp SJ, Salamini F, Gebhardt C (1994) Quantitative resistance to Phytophthora infestans in potato: a case study for QTL mapping in an allogamous plant species. Genetics 137:67–77

    Google Scholar 

  • Martinez-Zapater JM, Olivier JL (1985) Isozyme gene duplication in diploid and tetraploid potatoes. Theor Appl Genet 70:172–177

    Google Scholar 

  • Nap JP, Van Spanje M, Dirkse WG, Baarda G, Mlynarova L, Loonen A, Grondhuis P, Stiekema WJ (1993) Activity of the promoter of the Lhca3.St.1 gene, encoding the potato apoprotein 2 of the light-harvesting complex of Photosystem I, in transgenic potato and tobacco plants. Plant Mol Biol 23:605–612

    Google Scholar 

  • Osborne BI, Corr CA, Prince JP, Hehl R, Tanksley SD, McCormick S, Baker B (1991) Ac transposition from a T-DNA can generate linked and unlinked clusters of insertions in the tomato genome. Genetics 129:833–844

    Google Scholar 

  • Pereira A, Aarts M, Van Agtmaal S, Stiekema WJ, Jacobsen E (1991) Waxy variegation in transgenic potato. Maydica 36:323–327

    Google Scholar 

  • Pereira A, Jacobs JME, Te Lintel Hekkert W, Rutgers E, Jacobsen E, Stiekema WJ (1992) Towards the isolation of resistance genes by transposon targeting in potato. Neth J Plant Pathol 98 [Suppl 2]:215–221

    Google Scholar 

  • Ramanna MS, Wagenvoort M (1976) Identification of the trisomic series in diploid Solanum tuberosum L. Group Tuberosum. I. Chromosome identification. Euphytica 25:233–240

    Google Scholar 

  • Ritter E, Gebhardt C, Salamini F (1990) Estimation of recombination frequencies and construction of RFLP linkage maps in plants from crosses between heterozygous parents. Genetics 125:645–654

    CAS  PubMed  Google Scholar 

  • Simmonds NW (1965) Mutant expression in diploid potatoes. Heredity 20:65–72

    Google Scholar 

  • Stam P (1993) Construction of integrated genetic linkage maps by means of a new computer package: Joinmap. Plant J 3:739–744

    Article  CAS  Google Scholar 

  • Tanskley SD, Loaiza-Figueroa F (1985) Gametophytic self-incompatibility is controlled by a single major locus on chromosome 1 in Lycopersicon peruvianum. Proc Natl Acad Sci USA 82:5093–5096

    CAS  Google Scholar 

  • Tanksley SD, Ganal MW, Prince JP, De Vicente MC, Bonierbale MW, Broun P, Fulton TM, Giovannoni JJ, Grandillo S, Martin GB, Messeguer R, Miller JC, Miller L, Paterson AH, Pineda O, Röder MS, Wing RA, Wu W, Young ND (1992) High-density molecular linkage maps of the tomato and potato genomes. Genetics 132:1141–1160

    CAS  PubMed  Google Scholar 

  • Thompson RD, Uhrig H, Hermsen JGTh, Salamini F, Kaufmann H (1991) Investigation of a self-compatible mutation in Solanum tuberosum clones inhibiting S-allele activity in pollen differentially. Mol Gen Genet 226:283–288

    CAS  PubMed  Google Scholar 

  • Triglia T, Peterson MG, Kemp DJ (1988) A procedure for in vitro amplification of DNA segments that lie outside the boundaries of known sequences. Nucleic Acids Res 16:8186

    Google Scholar 

  • Vallejos CJ (1983) Enzyme activity stains. In: Tanksley SD, Orton TJ (eds) Isozymes in plant genetics and breeding, part A. Elsevier, Amsterdam New York, pp 469–516

    Google Scholar 

  • Van Eck HJ, Jacobs JME, Van Dijk J, Stiekema WJ, Jacobsen E (1993) Identification and mapping of three flower colour loci of potato (S. tuberosum L.) by RFLP analysis. Theor Appl Genet 86:295–300

    Google Scholar 

  • Van Eck HJ, Jacobs JME, Stam P, Ton J, Stiekema WJ, Jacobsen E (1994a) Multiple alleles for tuber shape in diploid potato detected by qualitative and quantitative genetic analysis using RFLPs. Genetics 137:303–309

    Google Scholar 

  • Van Eck HJ, Jacobs JME, Van den Berg PMMM, Stiekema WJ, Jacobsen E (1994b) The inheritance of anthocyanin pigmentation in potato (S. tuberosum L.) and mapping of tuber skin colour loci using RFLP's. Heredity 73:410–421

    Google Scholar 

  • Van Ooijen JW (1994) Drawmap: A computer program for drawing genetic linkage maps. J Hered 85:66

    Google Scholar 

  • Wagenvoort M (1982) Location of the recessive gene ym (yellow margin) on chromosome 12 of diploid Solanum tuberosum by means of trisomic analysis. Theor Appl Genet 61:239–243

    Google Scholar 

  • Walbot V (1992) Strategies for mutagenesis and gene cloning using transposon tagging and T-DNA insertional mutagenesis. Annu Rev Plant Physiol Plant Mol Biol 43:49–82

    Google Scholar 

  • Weide R, Van Wordragen MF, Klein Lankhorst R, Verkerk R, Hanhart C, Liharska T, Pap E, Stam P, Zabel P, Koornneef M (1993) Integration of the classical and molecular linkage maps of tomato chromosome 6. Genetics 135:1175–1186

    Google Scholar 

  • Young ND, Tanksley SD (1989) Restriction fragment length polymorphism maps and the concept of graphical genotypes. Theor Appl Genet 77:95–101

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Molecular Biology, DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, NL-6700, AA Wageningen, The Netherlands

    J. M. E. Jacobs, P. Arens, B. Verkerk-Bakker, B. te Lintel Hekkert, A. Pereira & W. J. Stiekema

  2. Department of Plant Breeding, Wageningen Agricultural University, P.O. Box 386, NL-6700, AJ Wageningen, The Netherlands

    H. J. Van Eck, H. J. M. Bastiaanssen, A. El-Kharbotly & E. Jacobsen

Authors
  1. J. M. E. Jacobs
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. J. Van Eck
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. P. Arens
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. B. Verkerk-Bakker
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. B. te Lintel Hekkert
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. H. J. M. Bastiaanssen
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. A. El-Kharbotly
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. A. Pereira
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. E. Jacobsen
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. W. J. Stiekema
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by F. Salamini

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jacobs, J.M.E., Van Eck, H.J., Arens, P. et al. A genetic map of potato (Solanum tuberosum) integrating molecular markers, including transposons, and classical markers. Theoret. Appl. Genetics 91, 289–300 (1995). https://doi.org/10.1007/BF00220891

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00220891

Key words

Search

Navigation