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  • 11
    Publication Date: 1990-05-25
    Description: An active site, cofactor-containing peptide has been obtained in high yield from bovine serum amine oxidase. Sequencing of this pentapeptide indicates: Leu-Asn-X-Asp-Tyr. Analysis of the peptide by mass spectrometry, ultraviolet-visible spectroscopy, and proton nuclear magnetic resonance leads to the identification of X as 6-hydroxydopa. This result indicates that, contrary to previous proposals, pyrroloquinoline quinone is not the active site cofactor in mammalian copper amine oxidases. Although 6-hydroxydopa has been implicated in neurotoxicity, the data presented suggest that this compound has a functional role at an enzyme active site.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Janes, S M -- Mu, D -- Wemmer, D -- Smith, A J -- Kaur, S -- Maltby, D -- Burlingame, A L -- Klinman, J P -- GM 39296/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 1990 May 25;248(4958):981-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Chemistry, University of California, Berkeley 94720.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2111581" target="_blank"〉PubMed〈/a〉
    Keywords: *Amine Oxidase (Copper-Containing) ; Amino Acid Sequence ; Animals ; Binding Sites ; Cattle ; Copper ; Dihydroxyphenylalanine/*analogs & derivatives/metabolism ; Magnetic Resonance Spectroscopy ; Mass Spectrometry ; Molecular Sequence Data ; Oxidoreductases/metabolism ; Oxidoreductases Acting on CH-NH Group Donors/blood/*metabolism ; Peptide Fragments/analysis/chemical synthesis ; Quinones/metabolism ; Spectrophotometry, Ultraviolet
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 12
    Publication Date: 2007-01-16
    Description: We describe the genome sequence of the protist Trichomonas vaginalis, a sexually transmitted human pathogen. Repeats and transposable elements comprise about two-thirds of the approximately 160-megabase genome, reflecting a recent massive expansion of genetic material. This expansion, in conjunction with the shaping of metabolic pathways that likely transpired through lateral gene transfer from bacteria, and amplification of specific gene families implicated in pathogenesis and phagocytosis of host proteins may exemplify adaptations of the parasite during its transition to a urogenital environment. The genome sequence predicts previously unknown functions for the hydrogenosome, which support a common evolutionary origin of this unusual organelle with mitochondria.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2080659/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉   〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2080659/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Carlton, Jane M -- Hirt, Robert P -- Silva, Joana C -- Delcher, Arthur L -- Schatz, Michael -- Zhao, Qi -- Wortman, Jennifer R -- Bidwell, Shelby L -- Alsmark, U Cecilia M -- Besteiro, Sebastien -- Sicheritz-Ponten, Thomas -- Noel, Christophe J -- Dacks, Joel B -- Foster, Peter G -- Simillion, Cedric -- Van de Peer, Yves -- Miranda-Saavedra, Diego -- Barton, Geoffrey J -- Westrop, Gareth D -- Muller, Sylke -- Dessi, Daniele -- Fiori, Pier Luigi -- Ren, Qinghu -- Paulsen, Ian -- Zhang, Hanbang -- Bastida-Corcuera, Felix D -- Simoes-Barbosa, Augusto -- Brown, Mark T -- Hayes, Richard D -- Mukherjee, Mandira -- Okumura, Cheryl Y -- Schneider, Rachel -- Smith, Alias J -- Vanacova, Stepanka -- Villalvazo, Maria -- Haas, Brian J -- Pertea, Mihaela -- Feldblyum, Tamara V -- Utterback, Terry R -- Shu, Chung-Li -- Osoegawa, Kazutoyo -- de Jong, Pieter J -- Hrdy, Ivan -- Horvathova, Lenka -- Zubacova, Zuzana -- Dolezal, Pavel -- Malik, Shehre-Banoo -- Logsdon, John M Jr -- Henze, Katrin -- Gupta, Arti -- Wang, Ching C -- Dunne, Rebecca L -- Upcroft, Jacqueline A -- Upcroft, Peter -- White, Owen -- Salzberg, Steven L -- Tang, Petrus -- Chiu, Cheng-Hsun -- Lee, Ying-Shiung -- Embley, T Martin -- Coombs, Graham H -- Mottram, Jeremy C -- Tachezy, Jan -- Fraser-Liggett, Claire M -- Johnson, Patricia J -- 072031/Wellcome Trust/United Kingdom -- G0000508/Medical Research Council/United Kingdom -- G0000508(56841)/Medical Research Council/United Kingdom -- G9722968/Medical Research Council/United Kingdom -- G9722968(65078)/Medical Research Council/United Kingdom -- R01 LM006845/LM/NLM NIH HHS/ -- R01 LM006845-08/LM/NLM NIH HHS/ -- R01 LM007938/LM/NLM NIH HHS/ -- R01 LM007938-04/LM/NLM NIH HHS/ -- U01 AI050913/AI/NIAID NIH HHS/ -- U01 AI050913-01A1/AI/NIAID NIH HHS/ -- U01 AI050913-02/AI/NIAID NIH HHS/ -- UO1 AI50913-01/AI/NIAID NIH HHS/ -- New York, N.Y. -- Science. 2007 Jan 12;315(5809):207-12.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Institute for Genomic Research, 9712 Medical Research Drive, Rockville, MD 20850, USA. jane.carlton@med.nyu.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/17218520" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Biological Transport/genetics ; DNA Transposable Elements ; DNA, Protozoan/genetics ; Gene Transfer, Horizontal ; Genes, Protozoan ; *Genome, Protozoan ; Humans ; Hydrogen/metabolism ; Metabolic Networks and Pathways/genetics ; Molecular Sequence Data ; Multigene Family ; Organelles/metabolism ; Oxidative Stress/genetics ; Peptide Hydrolases/genetics/metabolism ; Protozoan Proteins/genetics/physiology ; RNA Processing, Post-Transcriptional ; Repetitive Sequences, Nucleic Acid ; *Sequence Analysis, DNA ; Sexually Transmitted Diseases/parasitology ; Trichomonas Infections/parasitology/transmission ; Trichomonas vaginalis/cytology/*genetics/metabolism/pathogenicity
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 13
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 2010-06-12
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Smith, Andrew J -- Schwappach, Blanche -- New York, N.Y. -- Science. 2010 Jun 11;328(5984):1364-5. doi: 10.1126/science.1191529.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Faculty of Life Sciences, University of Manchester, Oxford Road, Manchester M13 9PT, UK.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/20538939" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Chloride Channels/genetics/*metabolism ; Chlorides/*metabolism ; Endosomes/*metabolism ; Genetic Engineering ; Hydrogen-Ion Concentration ; Kidney Diseases/metabolism ; Lysosomes/*metabolism ; Membrane Potentials ; Mice ; *Protons
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 14
    Publication Date: 2012-04-24
    Description: Cell transplantation is a potential strategy for treating blindness caused by the loss of photoreceptors. Although transplanted rod-precursor cells are able to migrate into the adult retina and differentiate to acquire the specialized morphological features of mature photoreceptor cells, the fundamental question remains whether transplantation of photoreceptor cells can actually improve vision. Here we provide evidence of functional rod-mediated vision after photoreceptor transplantation in adult Gnat1-/- mice, which lack rod function and are a model of congenital stationary night blindness. We show that transplanted rod precursors form classic triad synaptic connections with second-order bipolar and horizontal cells in the recipient retina. The newly integrated photoreceptor cells are light-responsive with dim-flash kinetics similar to adult wild-type photoreceptors. By using intrinsic imaging under scotopic conditions we demonstrate that visual signals generated by transplanted rods are projected to higher visual areas, including V1. Moreover, these cells are capable of driving optokinetic head tracking and visually guided behaviour in the Gnat1-/- mouse under scotopic conditions. Together, these results demonstrate the feasibility of photoreceptor transplantation as a therapeutic strategy for restoring vision after retinal degeneration.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3888831/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉   〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3888831/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Pearson, R A -- Barber, A C -- Rizzi, M -- Hippert, C -- Xue, T -- West, E L -- Duran, Y -- Smith, A J -- Chuang, J Z -- Azam, S A -- Luhmann, U F O -- Benucci, A -- Sung, C H -- Bainbridge, J W -- Carandini, M -- Yau, K-W -- Sowden, J C -- Ali, R R -- 082217/Wellcome Trust/United Kingdom -- EY016805/EY/NEI NIH HHS/ -- EY06837/EY/NEI NIH HHS/ -- EY11307/EY/NEI NIH HHS/ -- G03000341/Medical Research Council/United Kingdom -- G0901550/Medical Research Council/United Kingdom -- MR/J004553/1/Medical Research Council/United Kingdom -- NIHR-RP-011-003/Department of Health/United Kingdom -- R01 EY011307/EY/NEI NIH HHS/ -- R01 EY011307-18/EY/NEI NIH HHS/ -- R01 EY016805/EY/NEI NIH HHS/ -- R01 EY016805-07/EY/NEI NIH HHS/ -- Department of Health/United Kingdom -- England -- Nature. 2012 May 3;485(7396):99-103. doi: 10.1038/nature10997.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Genetics, UCL Institute of Ophthalmology, University College London, 11-43 Bath Street, London, EC1V 9EL, UK. rachael.pearson@ucl.ac.uk〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/22522934" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; GTP-Binding Protein alpha Subunits/deficiency/genetics ; Light ; Maze Learning ; Mice ; Retinal Bipolar Cells/ultrastructure ; Retinal Horizontal Cells/ultrastructure ; Retinal Rod Photoreceptor Cells/cytology/*physiology/radiation ; effects/*transplantation ; Transducin/deficiency/genetics ; Vision, Ocular/*physiology/radiation effects ; Visual Cortex/physiology/radiation effects
    Print ISSN: 0028-0836
    Electronic ISSN: 1476-4687
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
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  • 15
    Publication Date: 2013-04-20
    Description: Massive present-day early-type (elliptical and lenticular) galaxies probably gained the bulk of their stellar mass and heavy elements through intense, dust-enshrouded starbursts--that is, increased rates of star formation--in the most massive dark-matter haloes at early epochs. However, it remains unknown how soon after the Big Bang massive starburst progenitors exist. The measured redshift (z) distribution of dusty, massive starbursts has long been suspected to be biased low in z owing to selection effects, as confirmed by recent findings of systems with redshifts as high as ~5 (refs 2-4). Here we report the identification of a massive starburst galaxy at z = 6.34 through a submillimetre colour-selection technique. We unambiguously determined the redshift from a suite of molecular and atomic fine-structure cooling lines. These measurements reveal a hundred billion solar masses of highly excited, chemically evolved interstellar medium in this galaxy, which constitutes at least 40 per cent of the baryonic mass. A 'maximum starburst' converts the gas into stars at a rate more than 2,000 times that of the Milky Way, a rate among the highest observed at any epoch. Despite the overall downturn in cosmic star formation towards the highest redshifts, it seems that environments mature enough to form the most massive, intense starbursts existed at least as early as 880 million years after the Big Bang.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Riechers, Dominik A -- Bradford, C M -- Clements, D L -- Dowell, C D -- Perez-Fournon, I -- Ivison, R J -- Bridge, C -- Conley, A -- Fu, Hai -- Vieira, J D -- Wardlow, J -- Calanog, J -- Cooray, A -- Hurley, P -- Neri, R -- Kamenetzky, J -- Aguirre, J E -- Altieri, B -- Arumugam, V -- Benford, D J -- Bethermin, M -- Bock, J -- Burgarella, D -- Cabrera-Lavers, A -- Chapman, S C -- Cox, P -- Dunlop, J S -- Earle, L -- Farrah, D -- Ferrero, P -- Franceschini, A -- Gavazzi, R -- Glenn, J -- Solares, E A Gonzalez -- Gurwell, M A -- Halpern, M -- Hatziminaoglou, E -- Hyde, A -- Ibar, E -- Kovacs, A -- Krips, M -- Lupu, R E -- Maloney, P R -- Martinez-Navajas, P -- Matsuhara, H -- Murphy, E J -- Naylor, B J -- Nguyen, H T -- Oliver, S J -- Omont, A -- Page, M J -- Petitpas, G -- Rangwala, N -- Roseboom, I G -- Scott, D -- Smith, A J -- Staguhn, J G -- Streblyanska, A -- Thomson, A P -- Valtchanov, I -- Viero, M -- Wang, L -- Zemcov, M -- Zmuidzinas, J -- England -- Nature. 2013 Apr 18;496(7445):329-33. doi: 10.1038/nature12050.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉California Institute of Technology, 1200 East California Boulevard, MC 249-17, Pasadena, California 91125, USA. dr@astro.cornell.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/23598341" target="_blank"〉PubMed〈/a〉
    Print ISSN: 0028-0836
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  • 16
    Publication Date: 2012-09-11
    Description: Transcription-coupled DNA repair uses components of the transcription machinery to identify DNA lesions and initiate their repair. These repair pathways are complex, so their mechanistic features remain poorly understood. Bacterial transcription-coupled repair is initiated when RNA polymerase stalled at a DNA lesion is removed by Mfd, an ATP-dependent DNA translocase. Here we use single-molecule DNA nanomanipulation to observe the dynamic interactions of Escherichia coli Mfd with RNA polymerase elongation complexes stalled by a cyclopyrimidine dimer or by nucleotide starvation. We show that Mfd acts by catalysing two irreversible, ATP-dependent transitions with different structural, kinetic and mechanistic features. Mfd remains bound to the DNA in a long-lived complex that could act as a marker for sites of DNA damage, directing assembly of subsequent DNA repair factors. These results provide a framework for considering the kinetics of transcription-coupled repair in vivo, and open the way to reconstruction of complete DNA repair pathways at single-molecule resolution.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3475728/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉   〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3475728/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Howan, Kevin -- Smith, Abigail J -- Westblade, Lars F -- Joly, Nicolas -- Grange, Wilfried -- Zorman, Sylvain -- Darst, Seth A -- Savery, Nigel J -- Strick, Terence R -- BB/I001859/1/Biotechnology and Biological Sciences Research Council/United Kingdom -- BB/I003142/1/Biotechnology and Biological Sciences Research Council/United Kingdom -- GM073829/GM/NIGMS NIH HHS/ -- R01 GM073829/GM/NIGMS NIH HHS/ -- England -- Nature. 2012 Oct 18;490(7420):431-4. doi: 10.1038/nature11430. Epub 2012 Sep 9.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Institut Jacques Monod, CNRS, UMR 7592, University Paris Diderot, Sorbonne Paris Cite F-75205 Paris, France.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/22960746" target="_blank"〉PubMed〈/a〉
    Keywords: Adenosine Triphosphate/metabolism ; Bacterial Proteins/*metabolism ; Biocatalysis ; DNA Damage ; *DNA Repair ; DNA-Directed RNA Polymerases/metabolism ; Escherichia coli/enzymology/genetics/metabolism ; Hydrolysis ; Kinetics ; Promoter Regions, Genetic/genetics ; Pyrimidine Dimers/chemistry/metabolism ; Transcription Elongation, Genetic ; Transcription Factors/*metabolism ; Transcription Initiation, Genetic ; Transcription Termination, Genetic ; *Transcription, Genetic
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  • 17
    Publication Date: 2011-02-18
    Description: The extragalactic background light at far-infrared wavelengths comes from optically faint, dusty, star-forming galaxies in the Universe with star formation rates of a few hundred solar masses per year. These faint, submillimetre galaxies are challenging to study individually because of the relatively poor spatial resolution of far-infrared telescopes. Instead, their average properties can be studied using statistics such as the angular power spectrum of the background intensity variations. A previous attempt at measuring this power spectrum resulted in the suggestion that the clustering amplitude is below the level computed with a simple ansatz based on a halo model. Here we report excess clustering over the linear prediction at arcminute angular scales in the power spectrum of brightness fluctuations at 250, 350 and 500 mum. From this excess, we find that submillimetre galaxies are located in dark matter haloes with a minimum mass, M(min), such that log(10)[M(min)/M(middle dot in circle)] = 11.5(+0.7)(-0.2) at 350 mum, where M(middle dot in circle) is the solar mass. This minimum dark matter halo mass corresponds to the most efficient mass scale for star formation in the Universe, and is lower than that predicted by semi-analytical models for galaxy formation.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Amblard, Alexandre -- Cooray, Asantha -- Serra, Paolo -- Altieri, B -- Arumugam, V -- Aussel, H -- Blain, A -- Bock, J -- Boselli, A -- Buat, V -- Castro-Rodriguez, N -- Cava, A -- Chanial, P -- Chapin, E -- Clements, D L -- Conley, A -- Conversi, L -- Dowell, C D -- Dwek, E -- Eales, S -- Elbaz, D -- Farrah, D -- Franceschini, A -- Gear, W -- Glenn, J -- Griffin, M -- Halpern, M -- Hatziminaoglou, E -- Ibar, E -- Isaak, K -- Ivison, R J -- Khostovan, A A -- Lagache, G -- Levenson, L -- Lu, N -- Madden, S -- Maffei, B -- Mainetti, G -- Marchetti, L -- Marsden, G -- Mitchell-Wynne, K -- Nguyen, H T -- O'Halloran, B -- Oliver, S J -- Omont, A -- Page, M J -- Panuzzo, P -- Papageorgiou, A -- Pearson, C P -- Perez-Fournon, I -- Pohlen, M -- Rangwala, N -- Roseboom, I G -- Rowan-Robinson, M -- Portal, M Sanchez -- Schulz, B -- Scott, Douglas -- Seymour, N -- Shupe, D L -- Smith, A J -- Stevens, J A -- Symeonidis, M -- Trichas, M -- Tugwell, K -- Vaccari, M -- Valiante, E -- Valtchanov, I -- Vieira, J D -- Vigroux, L -- Wang, L -- Ward, R -- Wright, G -- Xu, C K -- Zemcov, M -- England -- Nature. 2011 Feb 24;470(7335):510-2. doi: 10.1038/nature09771. Epub 2011 Feb 16.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Physics & Astronomy, University of California, Irvine, California 92697, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/21326201" target="_blank"〉PubMed〈/a〉
    Print ISSN: 0028-0836
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  • 18
    Publication Date: 2012-05-12
    Description: The old, red stars that constitute the bulges of galaxies, and the massive black holes at their centres, are the relics of a period in cosmic history when galaxies formed stars at remarkable rates and active galactic nuclei (AGN) shone brightly as a result of accretion onto black holes. It is widely suspected, but unproved, that the tight correlation between the mass of the black hole and the mass of the stellar bulge results from the AGN quenching the surrounding star formation as it approaches its peak luminosity. X-rays trace emission from AGN unambiguously, whereas powerful star-forming galaxies are usually dust-obscured and are brightest at infrared and submillimetre wavelengths. Here we report submillimetre and X-ray observations that show that rapid star formation was common in the host galaxies of AGN when the Universe was 2-6 billion years old, but that the most vigorous star formation is not observed around black holes above an X-ray luminosity of 10(44) ergs per second. This suppression of star formation in the host galaxy of a powerful AGN is a key prediction of models in which the AGN drives an outflow, expelling the interstellar medium of its host and transforming the galaxy's properties in a brief period of cosmic time.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Page, M J -- Symeonidis, M -- Vieira, J D -- Altieri, B -- Amblard, A -- Arumugam, V -- Aussel, H -- Babbedge, T -- Blain, A -- Bock, J -- Boselli, A -- Buat, V -- Castro-Rodriguez, N -- Cava, A -- Chanial, P -- Clements, D L -- Conley, A -- Conversi, L -- Cooray, A -- Dowell, C D -- Dubois, E N -- Dunlop, J S -- Dwek, E -- Dye, S -- Eales, S -- Elbaz, D -- Farrah, D -- Fox, M -- Franceschini, A -- Gear, W -- Glenn, J -- Griffin, M -- Halpern, M -- Hatziminaoglou, E -- Ibar, E -- Isaak, K -- Ivison, R J -- Lagache, G -- Levenson, L -- Lu, N -- Madden, S -- Maffei, B -- Mainetti, G -- Marchetti, L -- Nguyen, H T -- O'Halloran, B -- Oliver, S J -- Omont, A -- Panuzzo, P -- Papageorgiou, A -- Pearson, C P -- Perez-Fournon, I -- Pohlen, M -- Rawlings, J I -- Rigopoulou, D -- Riguccini, L -- Rizzo, D -- Rodighiero, G -- Roseboom, I G -- Rowan-Robinson, M -- Sanchez Portal, M -- Schulz, B -- Scott, D -- Seymour, N -- Shupe, D L -- Smith, A J -- Stevens, J A -- Trichas, M -- Tugwell, K E -- Vaccari, M -- Valtchanov, I -- Viero, M -- Vigroux, L -- Wang, L -- Ward, R -- Wright, G -- Xu, C K -- Zemcov, M -- England -- Nature. 2012 May 9;485(7397):213-6. doi: 10.1038/nature11096.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Mullard Space Science Laboratory, University College London, Holmbury St Mary, Dorking, Surrey RH5 6NT, UK. mjp@mssl.ucl.ac.uk〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/22575961" target="_blank"〉PubMed〈/a〉
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  • 19
    Publication Date: 1996-05-10
    Description: The development of the thymus depends initially on epithelial-mesenchymal and subsequently on reciprocal lympho-stromal interactions. The genetic steps governing development and differentiation of the thymic microenvironment are unknown. With the use of a targeted disruption of the whn gene, which recapitulates the phenotype of the athymic nude mouse, the WHN transcription factor was shown to be the product of the nude locus. Formation of the thymic epithelial primordium before the entry of lymphocyte progenitors did not require the activity of WHN. However, subsequent differentiation of primitive precursor cells into subcapsular, cortical, and medullary epithelial cells of the postnatal thymus did depend on activity of the whn gene. These results define the first genetically separable steps during thymic epithelial differentiation.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Nehls, M -- Kyewski, B -- Messerle, M -- Waldschutz, R -- Schuddekopf, K -- Smith, A J -- Boehm, T -- New York, N.Y. -- Science. 1996 May 10;272(5263):886-9.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Deutsches Krebsforschungszentrum, Im Neuenheimer Feld, Heidelberg, Germany.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/8629026" target="_blank"〉PubMed〈/a〉
    Keywords: Alleles ; Animals ; Animals, Newborn ; Base Sequence ; Cell Differentiation/*genetics ; Crosses, Genetic ; DNA-Binding Proteins/*genetics/physiology ; Epithelial Cells ; Female ; Forkhead Transcription Factors ; Gene Expression Regulation, Developmental ; Gene Targeting ; Genetic Complementation Test ; Male ; Mice ; Mice, Nude ; Molecular Sequence Data ; T-Lymphocytes/*cytology ; Thymus Gland/*cytology/embryology/metabolism ; Transcription Factors/*genetics/physiology
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    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 20
    Publication Date: 1996-08-23
    Description: A previously unknown redox cofactor has been identified in the active site of lysyl oxidase from the bovine aorta. Edman sequencing, mass spectrometry, ultraviolet-visible spectra, and resonance Raman studies showed that this cofactor is a quinone. Its structure is derived from the crosslinking of the epsilon-amino group of a peptidyl lysine with the modified side chain of a tyrosyl residue, and it has been designated lysine tyrosylquinone. This quinone appears to be the only example of a mammalian cofactor formed from the crosslinking of two amino acid side chains. This discovery expands the range of known quino-cofactor structures and has implications for the mechanism of their biogenesis.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Wang, S X -- Mure, M -- Medzihradszky, K F -- Burlingame, A L -- Brown, D E -- Dooley, D M -- Smith, A J -- Kagan, H M -- Klinman, J P -- GM27659/GM/NIGMS NIH HHS/ -- GM39296/GM/NIGMS NIH HHS/ -- P41 RR01614/RR/NCRR NIH HHS/ -- etc. -- New York, N.Y. -- Science. 1996 Aug 23;273(5278):1078-84.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Chemistry, University of California, Berkeley, CA 94720, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/8688089" target="_blank"〉PubMed〈/a〉
    Keywords: Amino Acid Sequence ; Animals ; Aorta/enzymology ; Binding Sites ; Cattle ; Chromatography, High Pressure Liquid ; Lysine/*analogs & derivatives/chemistry/metabolism ; Mass Spectrometry ; Molecular Sequence Data ; Molecular Weight ; Mutagenesis, Site-Directed ; Oxidation-Reduction ; Protein-Lysine 6-Oxidase/*chemistry/genetics/isolation & purification/metabolism ; Quinones/*chemistry/metabolism ; Spectrophotometry, Ultraviolet ; Spectrum Analysis, Raman
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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