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  • Solanum tuberosum  (25)
  • Mutant  (3)
  • plant regeneration  (3)
  • 1
    Electronic Resource
    Electronic Resource
    Molecular cloning and partial characterization of the gene for granule-bound starch synthase from a wildtype and an amylose-free potato (Solanum tuberosum L.)
    Amsterdam : Elsevier
    Plant Science 64 (1989), S. 185-192 
    Details
    ISSN: 0168-9452
    Keywords: GBSS RNA ; Solanum tuberosum ; amylose-free potato ; granule-bound starch synthase genomic clones
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0168-9452(89)90023-X
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  • 2
    Electronic Resource
    Electronic Resource
    Efficient transformation of potato (Solanum tuberosum L.) using a binary vector in Agrobacterium rhizogenes (1989)
    Springer
    Theoretical and applied genetics 78 (1989), S. 594-600 
    Details
    ISSN: 1432-2242
    Keywords: Agrobacterium rhizogenes ; Solanum tuberosum ; Binary vector ; Neomycin phosphotransferase II ; β-Glucuronidase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We transformed three potato (Solanum tuberosum L.) genotypes by using A. rhizogenes or a mixture of A. rhizogenes and A. tumefaciens. Inoculations of potato stem segments were performed with Agrobacterium rhizogenes AM8703 containing two independent plasmids: the wild-type Ri-plasmid, pRI1855, and the binary vector plasmid, pBI121. In mixed inoculation experiments, Agrobacterium rhizogenes LBA1334 (pRI1855) and Agrobacterium tumefaciens AM8706 containing the disarmed Ti-plasmid (pAL4404) and the binary vector plasmid (pBI121) were mixed in a 1∶1 ratio. The T-DNA of the binary vector plasmid pBI121 contained two marker genes encoding neomycin phosphotransferase, which confers resistance to kanamycin, and β-glucuronidase. Both transformation procedures gave rise to hairy roots on potato stem segments within 2 weeks. With both procedures it was possible to obtain transformed hairy roots, able to grow on kanamycin and possessing β-glucuronidase activity, without selection pressure. The efficiency of the A. rhizogenes AM8703 transformation, however, was much higher than that of the “mixed” transformation. Up to 60% of the hairy roots resulting from the former transformation method were kanamycin resistant and possessed β-glucuronidase activity. There was no correlation between the height of the kanamycin resistance and that of the β-glucuronidase activity in a root clone. Hairy roots obtained from a diploid potato genotype turned out to be diploid in 80% of the cases. Transformed potato plants were recovered from Agrobacterium rhizogenes AM8703-induced hairy roots.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00290847
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  • 3
    Electronic Resource
    Electronic Resource
    Expression and inheritance of inserted markers in binary vector carrying Agrobacterium rhizogenes-transformed potato (Solanum tuberosum L.) (1989)
    Springer
    Theoretical and applied genetics 78 (1989), S. 705-714 
    Details
    ISSN: 1432-2242
    Keywords: Agrobacterium rhizogenes ; Solanum tuberosum ; Binary vector ; Neomycin phosphotransferase ; β-Glucuronidase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Transgenic shoots were regenerated from eight diploid potato hairy root clones obtained by transformation with Agrobacterium rhizogenes harboring next to its wild-type Ri-plasmid a binary vector containing the neomycin phosphotransferase and the β-glucuronidase genes. The plants exhibited the typical hairy root phenotype. Of the plants isolated, 58% were tetraploid and 38% were diploid. Flowering and tuberization was much better in the diploid than in the tetraploid plants. Transgenic plants formed a significantly larger root system when grown on kanamycin-containing medium as compared to growth on kanamycin-free medium. Direct evidence for genetic transformation was obtained by opine, neomycin phosphotransferase and β-glucuronidase assays, and by molecular hybridization. Fourteen flowering diploid plants were reciprocally crossed with untransformed S. tuberosum plants, but only six were successful. Seedlings obtained from four crosses showed that all traits were transmitted to the offspring. Molecular analysis confirmed the presence of multiple integrations (copies) of both vector T-DNA and Ri-T-DNA. The genetic data, furthermore, suggest that the traits derived from Ri-T-DNA and binary vector T-DNA are linked, as no recombination between the different traits was observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00262567
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  • 4
    Electronic Resource
    Electronic Resource
    Genetic localisation of transformation competence in diploid potato (1995)
    Springer
    Theoretical and applied genetics 91 (1995), S. 557-562 
    Details
    ISSN: 1432-2242
    Keywords: Solanum tuberosum ; Phytophthora infestans ; Transposable element ; Transformation competence ; Linkage analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In the course of improving diploid potato genotypes for transformation ability, selection for specific components affecting regeneration and transformation was carried out. From a segregating population between two good regenerating clones a selection was made to yield an optimal well-transforming and fertile genotype J92-6400-A16. This plant yielded predominantly diploid transformants and was heterozygous for the gene R1, conferring resistance to Phytophthora infestans. The speed of, and competence for, regeneration and transformation on both sides of the stem explant were improved. A competence factor for tranformation was found to be linked with the R1 locus and a molecular marker on chromosome 5. The male fertility of transformants was frequently decreased to a great extent, whereas female fertility was not so markedly affected.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223279
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  • 5
    Electronic Resource
    Electronic Resource
    R6 and R7 alleles of potato conferring race-specific resistance to Phytophthora infestans (Mont.) de Bary identified genetic loci clustering with the R3 locus on chromosome XI (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 880-884 
    Details
    ISSN: 1432-2242
    Keywords: Solanum tuberosum ; P. infestans ; Resistance ; Dihaploid induction ; RFLP mapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In potato, 11 resistance alleles (R1–R11) are known which confer race-specific resistance to the fungus Phytophthora infestans. R1 has been mapped previously to potato chromosome V and R3 to chromosome XI. Here we report on the localization of the R6 and R7 alleles on the genetic map of potato. Differential resistant strains of tetraploid Solanum tuberosum, clones MaR6 and MaR7, were used as parental plants for the parthenogenetic induction and selection of diploid genotypes containing the R6 or the R7 resistance allele to P. infestans. One resistant dihaploid from MaR7 could be used directly as a parent to produce diploid F1 progeny suitable for phenotypic and RFLP analysis. MaR6 did not produce useful dihaploids directly. After crossing MaR6 with a tetraploid susceptible genotype, resistant F1 clones were selected. The resistant genotypes were then used as parents for the induction of dihaploids. Six dihaploids bearing R6 were identified that could be crossed with a diploid susceptible genotype. Two diploid F1 populations, segregating for R6 and R7, respectively, were analysed with RFLP markers known to be linked with previously identified R genes. Markers linked with R3 were found also to be linked with R6 and R7. The resistance alleles R6 and R7 mapped to a similar distal position on chromosome XI as the R3 allele.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00221901
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  • 6
    Electronic Resource
    Electronic Resource
    Autotetraploids and genetic mapping using common AFLP markers: the R2 allele conferring resistance to Phytophthora infestans mapped on potato chromosome 4 (1998)
    Springer
    Theoretical and applied genetics 96 (1998), S. 1121-1128 
    Details
    ISSN: 1432-2242
    Keywords: Key words Tetrasomic inheritance ; Solanum tuberosum ; AFLP ; Bulked segregant analysis ; R2 resistance gene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Due to the complexity of tetrasomic inheritance, mapping studies in potato (Solanum tuberosum L.) are generally conducted at the diploid level. In the present study we tested the feasibility of Bulked Segregant Analysis (BSA) using a tetraploid offspring for the identification of AFLP markers linked to the R2 allele, which confers race-specific resistance to Phytophthora infestans. Eleven bulk-specific AFLP markers, detected in fingerprints of 205 AFLP primer combinations, could be mapped in a linkage group encompassing the R2 locus. The efficiency of BSA at the tetraploid level, determined by the frequency of single-dose restriction fragments (SDRF), was much higher than expected on the basis of overall genetic dissimilarity between the parental clones. The fortuitous detection of AFLPs with linkage to the R2 allele is explained on the basis of specific genetic dissimilarity between cultivated potato and the chromosomal segment introgressed from S. demissum carrying the resistant R2 allele. AFLP markers common to those with linkage to R2 were visually recognized by their electrophoretic mobility in the AFLP fingerprint in a parental clone of a reference mapping population. Using these common AFLP markers we anchored the linkage group comprising the R2 allele to potato chromosome 4.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050847
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  • 7
    Electronic Resource
    Electronic Resource
    Isolation of a nitrate reductase deficient mutant of Pisum sativum by means of selection for chlorate resistance (1980)
    Springer
    Theoretical and applied genetics 58 (1980), S. 39-42 
    Details
    ISSN: 1432-2242
    Keywords: Pea ; Chlorate resistance ; Nitrate reductase deficient ; Mutant
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary After EMS treatment of seeds of the Pisum variety ‘Rondo’ a chlorate resistant mutant was isolated which showed a decrease in the in vitro activity of the enzyme nitrate reductase of roughly 95%. The mutation is monogenic and recessive. The mutant shows a decrease in protein content, and an increase in the amount of nitrate accumulated and in the activity of the enzyme nitrite reductase. On a liquid nutrient medium containing nitrate as the sole nitrogen source and in soil, the mutant grows very poorly due to necrosis of the leaves. On liquid medium containing ammonium, either with or without nitrate, growth is as good as that of the parent variety.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00264667
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  • 8
    Electronic Resource
    Electronic Resource
    Isolation and characterization of potato-tomato somatic hybrids using an amylose-free potato mutant as parental genotype (1992)
    Springer
    Theoretical and applied genetics 85 (1992), S. 159-164 
    Details
    ISSN: 1432-2242
    Keywords: Solanum tuberosum ; Lycopersicon esculentum ; Protoplast fusion ; Amylose-free potato
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Using different genotypes of tomato and diploid potato, possessing alien selectable markers as well as endogenous markers, very high frequencies of protoplast fusion hybrids were obtained. One endogenous genetic marker, the amylose-free (amf) mutant of potato, was helpful not only for the confirmation of fusion products but also for the study of genetic complementation and the segregation of amylose-free starch in microspores. Cytological analysis of the fusion hybrids indicated that except for one which was hexaploid, all of them had a perfectly balanced chromosome number of allotetraploid constitution (2n = 4x = 48). Despite normal chromosome pairing and a diploid behaviour, the microspores in some of the fusion hybrids segregated for the recessive amf-locus. This anomalous segregation of a recessive character in these hybrids was shown not to be due to chromosome elimination or to the absence of the wild-type tomato Amf gene. Although all fusion hybrids were totally sterile, the hexaploid produced stainable pollen and berries with badly developed seeds. Embryo rescue has so far failed to produce backcross progeny.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222854
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  • 9
    Electronic Resource
    Electronic Resource
    Modification of symbiotic interaction of pea (Pisum sativum L.) andRhizobium leguminosarum by induced mutations (1984)
    Springer
    Plant and soil 82 (1984), S. 427-438 
    Details
    ISSN: 1573-5036
    Keywords: Mutant ; Mutation ; Nitrate ; Nitrate reductase ; Nodulation ; Pisum sativum L. ; Rhizobium leguminosarum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In pea (Pisum sativum L.), mutants could be induced, modified in the symbiotic interaction withRhizobium leguminosarum. Among 250 M2-families, two nodulation resistant mutants (K5 and K9) were obtained. In mutant K5 the nodulation resistance was monogenic recessive and not Rhizobium strain specific. Out of 220 M2-families one mutant nod3 was found which could form nodules at high nitrate concentrations (15 mM KNO3). This mutant nodulated abundantly with severalRhizobium strains, both in the absence and presence of nitrate. Probably as the result of a pleiotropic effect, its root morphology was also changed. Among 1800 M2-families, five nitrate reductase deficient mutants were obtained and one of them (mutant E1) was used to study the inhibitory effect of nitrate on nodulation and nitrogen fixation. The results of the present investigation show that pea mutants which are modified in their symbiosis withRhizobium leguminosarum, can readily be obtained. The significance of such mutants for fundamental studies of the legume-Rhizobium symbiosis and for applications in plant breeding is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02184280
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  • 10
    Electronic Resource
    Electronic Resource
    Phenotypic and genotypic characterization of an amylose-free starch mutant of the potato (1989)
    Springer
    Euphytica 44 (1989), S. 43-48 
    Details
    ISSN: 1573-5060
    Keywords: Solanum tuberosum ; potato ; mutant ; starch composition ; amylose-free ; embryo rescue ; inheritance ; phenotype ; breeding ; karyotype
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The amylose-free (amf) potato mutant 86.040 has been characterized phenotypically and genotypically. Not only storage starch in tubers and metabolic starch in leaves but also starch in cells with specific functions, such as columella cells in the root cap and guard cells of stomata, was amylose-free. Doubled amf-clones of 86.040 flowered well, but the percentage of stainable pollen was low (8.2%). Therefore, they were used as female parent in crosses with diploid amylose-containing plants. Over 400 pollinations gave 13 berries, all dropping off prematurely; they contained 78 ovules with embryos at different developmental stages. Twenty F1 plants were obtained by embryo rescue, all having amylose starch. Three of them were characterized more extensively. They flowered well and pollen stainabilitity as well as seed set were high. Analysis of the F1 plants and of F1 × F1 progeny plants showed that the amf-character is monogenic and recessive. The locus involved was designated amf +and the mutated allele amf.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00022597
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