Publikationsdatum:
2013-03-23
Beschreibung:
Developmental signals such as Wnts are often presented to cells in an oriented manner. To examine the consequences of local Wnt signaling, we immobilized Wnt proteins on beads and introduced them to embryonic stem cells in culture. At the single-cell level, the Wnt-bead induced asymmetric distribution of Wnt-beta-catenin signaling components, oriented the plane of mitotic division, and directed asymmetric inheritance of centrosomes. Before cytokinesis was completed, the Wnt-proximal daughter cell expressed high levels of nuclear beta-catenin and pluripotency genes, whereas the distal daughter cell acquired hallmarks of differentiation. We suggest that a spatially restricted Wnt signal induces an oriented cell division that generates distinct cell fates at predictable positions relative to the Wnt source.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3966430/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3966430/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Habib, Shukry J -- Chen, Bi-Chang -- Tsai, Feng-Chiao -- Anastassiadis, Konstantinos -- Meyer, Tobias -- Betzig, Eric -- Nusse, Roel -- 102513/Wellcome Trust/United Kingdom -- GM063702/GM/NIGMS NIH HHS/ -- NS069375/NS/NINDS NIH HHS/ -- R01 GM030179/GM/NIGMS NIH HHS/ -- R01 GM063702/GM/NIGMS NIH HHS/ -- Howard Hughes Medical Institute/ -- New York, N.Y. -- Science. 2013 Mar 22;339(6126):1445-8. doi: 10.1126/science.1231077.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Developmental Biology, Howard Hughes Medical Institute, Institute for Stem Cell Biology and Regenerative Medicine, Stanford University, 265 Campus Drive, Stanford, CA 94305, USA. shabib@stanford.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/23520113" target="_blank"〉PubMed〈/a〉
Schlagwort(e):
Animals
;
*Asymmetric Cell Division
;
*Cell Differentiation
;
Cells, Cultured
;
Centrosome/physiology
;
Cytokinesis
;
Embryonic Stem Cells/*cytology/*metabolism
;
Gene Expression
;
Homeodomain Proteins/genetics/metabolism
;
Mice
;
Mitosis
;
Octamer Transcription Factor-3/genetics/metabolism
;
Pluripotent Stem Cells/physiology
;
Recombinant Proteins/metabolism
;
Single-Cell Analysis
;
Transcription Factors/genetics/metabolism
;
Wnt Proteins/metabolism
;
*Wnt Signaling Pathway
;
Wnt3A Protein/*metabolism
;
beta Catenin/metabolism
Print ISSN:
0036-8075
Digitale ISSN:
1095-9203
Thema:
Biologie
,
Chemie und Pharmazie
,
Informatik
,
Medizin
,
Allgemeine Naturwissenschaft
,
Physik
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