Publication Date:
2009-07-04
Description:
Recent reports of mild to severe influenza-like illness in humans caused by a novel swine-origin 2009 A(H1N1) influenza virus underscore the need to better understand the pathogenesis and transmission of these viruses in mammals. In this study, selected 2009 A(H1N1) influenza isolates were assessed for their ability to cause disease in mice and ferrets and compared with a contemporary seasonal H1N1 virus for their ability to transmit to naive ferrets through respiratory droplets. In contrast to seasonal influenza H1N1 virus, 2009 A(H1N1) influenza viruses caused increased morbidity, replicated to higher titers in lung tissue, and were recovered from the intestinal tract of intranasally inoculated ferrets. The 2009 A(H1N1) influenza viruses exhibited less efficient respiratory droplet transmission in ferrets in comparison with the highly transmissible phenotype of a seasonal H1N1 virus. Transmission of the 2009 A(H1N1) influenza viruses was further corroborated by characterizing the binding specificity of the viral hemagglutinin to the sialylated glycan receptors (in the human host) by use of dose-dependent direct receptor-binding and human lung tissue-binding assays.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2953552/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2953552/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Maines, Taronna R -- Jayaraman, Akila -- Belser, Jessica A -- Wadford, Debra A -- Pappas, Claudia -- Zeng, Hui -- Gustin, Kortney M -- Pearce, Melissa B -- Viswanathan, Karthik -- Shriver, Zachary H -- Raman, Rahul -- Cox, Nancy J -- Sasisekharan, Ram -- Katz, Jacqueline M -- Tumpey, Terrence M -- GM 57073/GM/NIGMS NIH HHS/ -- R01 GM057073/GM/NIGMS NIH HHS/ -- R01 GM057073-09/GM/NIGMS NIH HHS/ -- R37 GM057073/GM/NIGMS NIH HHS/ -- U54 GM062116/GM/NIGMS NIH HHS/ -- U54 GM062116-09/GM/NIGMS NIH HHS/ -- U54 GM62116/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2009 Jul 24;325(5939):484-7. doi: 10.1126/science.1177238. Epub 2009 Jul 2.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19574347" target="_blank"〉PubMed〈/a〉
Keywords:
Adult
;
Animals
;
Disease Models, Animal
;
Female
;
Ferrets
;
Hemagglutinin Glycoproteins, Influenza Virus/chemistry/metabolism
;
Humans
;
Influenza A Virus, H1N1 Subtype/*pathogenicity
;
Influenza, Human/transmission/*virology
;
Intestines/virology
;
Male
;
Mice
;
Mice, Inbred BALB C
;
Models, Molecular
;
Orthomyxoviridae Infections/*transmission/*virology
;
Protein Binding
;
Receptors, Virus/metabolism
;
Respiratory System/virology
;
Swine
;
Virus Replication
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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