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  • Lymnaea Stagnalis  (1)
  • Lymnaea stagnalis  (1)
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  • 1
    ISSN: 1432-0878
    Schlagwort(e): Immunocytochemistry ; ACTH ; Lymnaea stagnalis ; Neurohormone ; Neurotransmitter
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Two giant neurons (diameter 130 μm) were identified immunocytochemically by means of the unlabeled antibody enzyme technique with anti ACTH 1–39 and 1–24 in the pond snail Lymnaea stagnalis. The cells are located in the visceral and the right parietal ganglion, respectively. They contain moderately electron dense elementary granules (diameter 150–160 nm). By means of the intracellular horseradish peroxidase injection technique it was shown that the cells send fibres into the neuropiles of various ganglia and into nerves. Synapses occur on the fine fibre branches in the neuropile. Synapse-like structures were found on the cell bodies and on the major fibres. The giant neurons are electrotonically coupled. With toluidine blue staining for small peptides it was demonstrated that in the central nervous system of the pond snail numerous peptidergic neurons occur in addition to those identified with the classical staining methods for neurosecretion.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    ISSN: 1432-0878
    Schlagwort(e): HRP injection ; Lymnaea Stagnalis ; Neurohaemal areas ; Neurosecretory cell ; Ultrastructure
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The paired, electrotonically coupled neurosecretory Canopy Cells (CC) of the pond snail Lymnaea stagnalis were microiontophoretically injected with horseradish peroxidase (HRP). Whole mount preparations and ultrathin sections of injected CC were studied to describe in detail the morphology of the CC, their axon tracts and neurohaemal areas. The CC release their secretory product at three different sites, viz. from the soma and from axon terminals in the intercerebral commissure and in the median lip nerve. Neural control over the CC occurs by few synapses found exclusively on the CC axon, not on the cell body. One type of “en passant” synapse was identified. Two morphologically distinct types of synapselike structures (SLS) are numerous. The site of electrotonic coupling between the two CC is most probably located in the cerebral commissure. Serial sectioning showed that the axons contact each other over more than 130 μm. The contact is very intimate and the axon membranes interdigitate in a complex manner. Gap junctions, which are often described as the sites of electrotonic coupling, were not found.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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