Summary
Two giant neurons (diameter 130 μm) were identified immunocytochemically by means of the unlabeled antibody enzyme technique with anti ACTH 1–39 and 1–24 in the pond snail Lymnaea stagnalis. The cells are located in the visceral and the right parietal ganglion, respectively. They contain moderately electron dense elementary granules (diameter 150–160 nm). By means of the intracellular horseradish peroxidase injection technique it was shown that the cells send fibres into the neuropiles of various ganglia and into nerves. Synapses occur on the fine fibre branches in the neuropile. Synapse-like structures were found on the cell bodies and on the major fibres. The giant neurons are electrotonically coupled. With toluidine blue staining for small peptides it was demonstrated that in the central nervous system of the pond snail numerous peptidergic neurons occur in addition to those identified with the classical staining methods for neurosecretion.
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References
Barry, J., Dubois, M.P.: Etude en immunofluorescence des structures hypothalamiques à compétence gonadotrope. Ann. Endocrinol. (Paris) 34, 735–742 (1973)
Benjamin, P.R., Swindale, N.V.: Electrical properties of “dark green” and “yellow” neurosecretory cells in the snail Lymnaea stagnalis L. Nature 258, 622–623 (1975)
Blankenship, J.E., Haskins, J.T.: Electrotonic coupling among neuroendocrine cells in Aplysia. J. Neurophysiol. 42, 356–367 (1977)
Boer, H.H., Roubos, E.W., Dalen, H. van, Groesbeek, J.R.F.Th.: Neurosecretion in the basommatophoran snail Bulinus truncatus (Gastropoda, Pulmonata). Cell Tissue Res. 176, 57–67 (1977)
Buijs, R.M.: Intra and extrahypothalamic vasopressin and oxytocin pathways in the rat. Pathways to the limbic system, medulla oblongata and spinal cord. Cell Tissue Res. 192, 423–435 (1978)
Buijs, R.M., Swaab, D.F.: Immunoelectronmicroscopical demonstration of vasopressin and oxytocin synapses in the rat limbic system. Cell Tissue Res. (submitted) (1980)
Buijs, R.M., Swaab, D.F., Dogterom, J., van Leeuwen, F.: Intra and extrahypothalamic vasopressin and oxytocin pathways in the rat. Cell Tissue Res. 186, 423–433 (1978)
Donk, J.A. van der, Lips, C.J.M., Hackeng, W.H.L., Dam, R.H. van, Goudswaard, J.: The synthesis of calcitonin: some characteristics of the precursors in man and rat. J. Molec. Medic. 3, 95–104 (1978)
Dunn, A.J., Green, E.J., Isaacson, R.L.: Intracerebral adrenocorticotropic hormone mediates noveltyinduced grooming in the rat. Science 203, 281–283 (1979)
Fritsch, H.A.R., Sprang, R.E.: On the ultrastructure of polypeptide hormone-producing cells in the gut of the ascidian Ciona intestinalis L. and the bivalve Mytilus edulis L. Cell Tissue Res. 177, 407–413 (1977)
Grimm-Jørgensen, Y.: Immunoreactive thyrotropin-releasing factor in a gastropod. Distribution in the central nervous system and haemolymph of Lymnaea stagnalis. Gen. Comp. Endocrinol. 35, 387–390 (1978)
Guillemin, R.: Peptides in the brain: the new endocrinology of the neuron. Science 202, 390–402 (1978)
Hökfelt, T., Efendic, D., Hellerström, C., Johansson, O., Luft, R., Arimura, A.: Cellular localization of somatostatin in endocrine-like cells and neurons of the rat with special references to the A1-cells of the pancreatic islets and to the hypothalamus. Acta Endocrinol. (Kbh.) 80 (Suppl. 200), 1–41 (1975)
Kiss, I., Benedeczky, I.: Physiological and ultrastructural investigations of an identified neurosecretory cell of Lymnaea stagnalis. Acta Biol. Sci. Hung. 28, 355–360 (1977)
Kiss, L., Salánki, J.: Examination of some morphological properties and axonal pathways of giant neurones in the central nervous system of Lymnaea stagnalis. Comp. Biochem. Physiol. (A) 57, 107–114 (1977)
Lips, C.J.M., Sluijs Veer, J. van der, Donk, J.A. van der, Dam, R.H. van, Hackeng, W.H.L.: Common precursor molecule as origin for the ectopic-hormone-producing-tumour syndrome. Lancet, Jan. 7, pp 16–18 (1978)
Mattheij, J.A.M.: The cell types in the adenohypophysis of the blind mexican cave fish Anoptichthys jordani (Hubbs and Innes). Z. Zellforsch. 90, 542–553 (1968)
Pearse, A.G.E.: The cytochemistry and ultrastructure of polypeptide hormone-producing cells of the APUD series and the embryologic, physiologic and pathologic implications of the concept. J. Histochem. Cytochem. 17, 303–313 (1969)
Pearse, A.G.E., Takor Takor, T.: Neuroendocrine embryology and the APUD concept. Clin. Endocrinol. 5, Suppl., 229–244 (1976)
Plesch, B.: An ultrastructural study of the innervation of the musculature of the pond snail Lymnaea stagnalis (L.) with reference to peripheral neurosecretion. Cell Tissue Res. 183, 353–369 (1977)
Pogorelaya, N.K., Elekes, K., Kiss, I.: Electron microscopic investigation of a giant neuron identified in the right parietal ganglion of Lymnaea stagnalis (L.) Acta Biol. Sci. Hung. 28, 451–460 (1977)
Rémy, C., Girardie, J., Dubois, M.P.: Exploration immunocytologiques des ganglions cérébroides et sous-oesphagien du phasme Clitumnus extradentatus: existence d'une neurosécrétion apparantée à la vasopressine-neurophysine. C.R. Acad. Sci. Paris, 285D, 1495–1497 (1977)
Rémy, C., Girardie, J., Dubois, M.P.: Vertebrate neuropeptide-like substances in the sub-oesophageal ganglion of two insects: Locusta migratoria R. and F. (Orthoptera) and Bombyx mori L. (Lepidoptera). Immunocytological investigation. Gen. Comp. Endocrinol. 37, 93–100 (1979)
Roubos, E.W.: Neuronal and non-neuronal control of the neurosecretory caudo-dorsal cells of the freshwater snail Lymnaea stagnalis (L.). Cell Tissue Res. 168, 11–31 (1976)
Scharrer, B.: Peptidergic neurons: facts and trends. Gen. Comp. Endocrinol. 34, 50–62 (1978)
Solcia, E., Vasallo, G., Capella, C.: Selective staining of endocrine cells by basic dyes after acid hydrolysis. Stain Technol. 43, 257–263 (1968)
Sternberger, L.A.: Immunocytochemistry. Foundation of immunology series (A. Osler and L. Weiss, eds.). Englewood Cliffs, New Jersey: Prentice Hall, Inc. 1974
Strambi, C., Strambi, A., Cupo, A., Rougon-Rapuzzi, G., Martin, N.: Étude des taux d'une substance apparantée à la vasopressine dans le système nerveux de Grillons à différentes conditions hygrométriques. C.R. Acad. Sci. Paris, 287D, 1227–1230 (1978)
Strambi, C., Rougon-Rapuzzi, G., Cupo, A., Martin, N., Strambi, A.: Mise en évidence immunocytologique d'un composé apparenté à la vasopressine dans le système nerveux du Grillon Acheta domesticus. C.R. Acad. Sci. Paris, 288D, 131–133 (1979)
Straus, E., Yalow, R.S., Gainer, H.: Molluscan gastrin: concentration and molecular forms. Science 190, 687–689 (1975)
Stretton, A.O.W., Kravitz, E.A.: Neuronal geometry: determination with a technique of intracellular dye injection. Science 162, 132–134 (1968)
Swaab, D.F.: Neuropeptides and brain development. A working hypothesis. In: A multidisciplinary approach to brain development. Proc. Internat. Meeting, Selva di Fasano, Italy, April 18–20, 1979. (C. Di Benedetta, ed.) (In press) (1979)
Swaab, D.F., Fisser, B.: Immunocytochemical localization of α-melanocyte stimulating hormone (α-MSH)-like compounds in the rat nervous system. Neurose. Letters 7, 313–317 (1978)
Swaab, D.F., Pool, C.W., Leeuwen, F. van: Can specificity ever be proved in immunocytochemical staining? J. Histochem. Cytochem. 25, 388–391 (1977)
Visser, M., Swaab, D.F.: Life span changes in the presence of α-MSH containing cells in the human pituitary. J. Developm. Physiol. 1 (In press) (1979)
Vlieger, T.A. de, Kits, K.S., Maat, A. ter, Lodder, J.C.: Morphology and electrophysiology of the ovulation hormone producing neuroendocrine cells of the freshwater snail Lymnaea stagnalis (L.). J. Exp. Biol. (In press) (1979)
Wendelaar Bonga, S.E.: Ultrastructure and histochemistry of neurosecretory cells and neurohaemal areas in the pond snail Lymnaea stagnalis (L.). Z. Zellforsch. 108, 190–224 (1970)
Wied, D. de, Gispen, W.H.: Behavioral effects of peptides.In: Peptides in Neurobiology (H. Gainer, ed.) pp. 397–448. New York and London: Plenum Press 1977
Winlow, W., Benjamin, P.R.: Neuronal mapping of the pond snail, Lymnaea stagnalis (L.). Neurobiology of Invertebrates. Gastropoda Brain. Tihany, 1975, pp. 41–59, 1976
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The authors wish to thank Prof. J. Lever for reading the manuscript and Mr. G.W.H. van den Berg for drawing Fig. 13
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Boer, H.H., Schot, L.P.C., Roubos, E.W. et al. ACTH-Iike immunoreactivity in two electrotonically coupled giant neurons in the pond snail Lymnaea stagnalis . Cell Tissue Res. 202, 231–240 (1979). https://doi.org/10.1007/BF00232237
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DOI: https://doi.org/10.1007/BF00232237