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  • 1
    Electronic Resource
    Electronic Resource
    Calcium regulation in the human myocardium affected by dilated cardiomyopathy: A structural basis for impaired Ca2+-sensitivity (1999)
    Springer
    Molecular and cellular biochemistry 194 (1999), S. 301-313 
    Details
    ISSN: 1573-4919
    Keywords: ATPase activity ; cardiomyopathy ; heart failure ; myosin light chains ; troponin-tropomyosin ; mekratin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Calcium regulation in the human heart is impaired during idiopathic dilated cardiomyopathy (IDC). Here, we analyze the structural basis for impairment in the regulatory mechanism. Regulation of contractility was monitored by MgATPase and Ca2+-binding assays as a function of calcium. Myofibrillar proteolysis and expression of troponin T isoforms were established by gel electrophoresis and by Western blots. Myofibrillar ATPase assays in low salt however, revealed a drastic lowering of calcium sensitivity in IDC myofibrils as indicated by reductions in both activation by high calcium and in EGTA-mediated inhibition of MgATPase. Structural changes in myofilament proteins were found in most IDC hearts, specifically proteolysis of myosin light chain 2 (LC2), troponin T and I (TnT and TnI), and sometimes large isoform shift in TnT. IDC did not induce mutations in LC2 and troponin C (TnC), as established by cDNA sequence data from IDC cases, thus, calcium binding to IDC myofibrils was unaffected. Reassociation of IDC myofibrils with native LC2 raised MgATPase activation at high Ca2+ to control levels, while repletion with intact, canine TnI/TnT restored inhibition at low Ca2+. A model, identifying possible steps in the steric blocking mechanism of regulation, is proposed to explain IDC-induced changes in Ca2+-regulation. Moreover, shifts in TnT isoforms may imply either a genetic or a compensatory factor in the development and pathogenesis of some forms of IDC.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006980405359
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Myofibrillar protein structure and assembly during idiopathic dilated cardiomyopathy (1999)
    Springer
    Molecular and cellular biochemistry 195 (1999), S. 1-10 
    Details
    ISSN: 1573-4919
    Keywords: myosin light chains ; native thick filaments ; dilated cardiomyopathy ; heart failure ; immunohistochemistry ; mekratin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract A neutral protease, mekratin, active in human hearts at end stage idiopathic dilated cardiomyopathy (IDC), mediates the breakdown of cardiac myosin LC2. Myosin purified from IDC heart tissue forms unusually short synthetic thick filaments. Therefore, determination of filament length and mekratin distribution in IDC heart muscle were initiated. Native thick filaments were prepared directly from control and IDC tissues and analyzed. Also, paraffin-embedded tissue sections were stained with a fluorescently-labeled anti-protease antibody to establish its distribution in myocardial tissues. Control sections had only very weak, background levels of fluorescence whereas IDC sections stained intensely throughout, indicating a wide ranging distribution of the protease within the myocyte cytoplasm. SDS-PAGE revealed LC2 to be present in stoichiometric amounts in control but greatly reduced in IDC heart muscle. Native thick filaments from control myocardium were structurally stable. They had a median length of 1.65 μm with well-defined bare zones and displayed the 43 nm helical periodicity typical of the relaxed arrangement of myosin heads close to the filaments' shafts. In contrast, native IDC filaments were less stable, and had a median length of 0.9 μm. These filaments were highly disordered: they had no surface periodicity and myosin heads were positioned away from the filaments' shafts. The shorter, less stable, aperiodic thick filaments from IDC hearts appear to result from depletion of LC2 caused by increased activity of mekratin in the IDC myocardium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006940513097
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  • 3
    Electronic Resource
    Electronic Resource
    Cloning of the cDNA and nucleotide sequence of a skeletal muscle protease from myopathic hamsters (1998)
    Springer
    Molecular and cellular biochemistry 181 (1998), S. 125-135 
    Details
    ISSN: 1573-4919
    Keywords: cardiomyopathy ; serine proteases ; myosin light chain 2 ; cDNA cloning ; mast cells ; mekratin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract A neutral protease with an estimated Mr of about 26 kD and responsible for cleavage of myosin LC2 was isolated from hamster skeletal muscle. Complementary DNAs were generated by RT-PCR using total hamster muscle RNA and degenerate oligonucleotide primers based on the sequences of two internal peptides. The nucleotide sequences of the resultant cDNAs were subsequently determined and the complete amino acid sequence of the protease deduced. Although the hamster protein shared 63-85% identity in nucleotide and amino acid sequences with rat and mouse mast cell proteases, it had a higher degree of specificity for myosin LC2 than mast cell proteases which also digested myosin LC1 and myosin heavy chains. As a result, the hamster protease was designated mekratin because of its unique enzymatic specificities to distinguish it from other mast cell proteases. A polyclonal antibody was raised specific to the hamster muscle and human cardiac muscle mekratins without apparent cross-reaction with rat mast cell proteases. We have earlier demonstrated the presence in excess of a neutral protease that specifically cleaves LC2 in human hearts obtained at end stage idiopathic dilated cardiomyopathy (IDC). Western analyses revealed that heart tissue from patients with IDC contained 5-10 fold more mekratin than control samples. Furthermore, the level of the protease in human IDC tissues was similar to that seen in myopathic hamster skeletal muscle. No bands were recognized by the antibody when IDC myofibrils were probed due to the removal of soluble proteins during sample preparation. Thus, these results strongly suggest that the anti-mekratin antibody will provide positive identification of IDC in many cases and diagnosis by exclusion may be replaced.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006842332340
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  • 4
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    Use of Smoothed Measured Winds to Predict and Assess Launch Environments (2011)
    In:  CASI
    Details
    Publication Date: 2019-07-13
    Description: Since many of the larger launch vehicles are operated near their design limits during the ascent phase of flight to optimize payload to orbit, it often becomes necessary to verify that the vehicle will remain within certification limits during the ascent phase as part of the go/no-go review made prior to launch. This paper describes the approach used to predict Ares I-X launch vehicle structural air loads and controllability prior to launch which represents a distinct departure from the methodology of the Space Shuttle and Evolved Expendable Launch Vehicle (EELV) programs. Protection for uncertainty of key environment and trajectory parameters is added to the nominal assessment of launch capability to ensure that critical launch trajectory variables would be within the integrated vehicle certification envelopes. This process was applied by the launch team as a key element of the launch day go/no-go recommendation. Pre-launch assessments of vehicle launch capability for NASA's Space Shuttle and the EELV heavy lift versions require the use of a high-resolution wind profile measurements, which have relatively small sample size compared with low-resolution profile databases (which include low-resolution balloons and radar wind profilers). The approach described in this paper has the potential to allow the pre-launch assessment team to use larger samples of wind measurements from low-resolution wind profile databases that will improve the accuracy of pre-launch assessments of launch availability with no degradation of mission assurance or launch safety.
    Keywords: Launch Vehicles and Launch Operations
    Type: JSC-CN-24016 , AIAA Atmospheric Flight Mechanics Conference; Aug 08, 2011 - Aug 11, 2011; Portland, OR; United States
    Format: application/pdf
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    NASA TECHNICAL REPORTS
  • 5
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    Use of Smoothed Measured Winds to Predict and Assess Launch Environments (2011)
    In:  CASI
    Details
    Publication Date: 2019-07-13
    Description: This paper describes an approach which was used to predict Ares I-X launch vehicle structural airloads and controllability prior to launch. This prediction along with the proper application of protection for variations in key environment and trajectory parameters was used to predict the potential for the launch trajectory to stay within the integrated vehicle certification envelopes. This data was applied by the launch team as a key element of the launch day go/no-go recommendation. NASA s Space Shuttle and the heavy lift versions of the Evolved Expendable Launch Vehicles assessments require the use of a high fidelity wind measurement which limits the number of wind measurement sources which can be used for a pre-launch go/no-go assessment. The approach described in this paper has the potential to allow the pre-launch assessment team to use wind measurements from a number of additional sources which will enhance launch availability and also has the potential to improve mission assurance and enhance safety
    Keywords: Launch Vehicles and Launch Operations
    Type: JSC-CN-22564 , JSC-CN-24381 , AIAA Atmospheric Flight Mechanics Conference; Aug 08, 2011 - Aug 11, 2011; Portland, OR; United States
    Format: application/pdf
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    NASA TECHNICAL REPORTS
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