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Myofibrillar protein structure and assembly during idiopathic dilated cardiomyopathy

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Abstract

A neutral protease, mekratin, active in human hearts at end stage idiopathic dilated cardiomyopathy (IDC), mediates the breakdown of cardiac myosin LC2. Myosin purified from IDC heart tissue forms unusually short synthetic thick filaments. Therefore, determination of filament length and mekratin distribution in IDC heart muscle were initiated. Native thick filaments were prepared directly from control and IDC tissues and analyzed. Also, paraffin-embedded tissue sections were stained with a fluorescently-labeled anti-protease antibody to establish its distribution in myocardial tissues. Control sections had only very weak, background levels of fluorescence whereas IDC sections stained intensely throughout, indicating a wide ranging distribution of the protease within the myocyte cytoplasm. SDS-PAGE revealed LC2 to be present in stoichiometric amounts in control but greatly reduced in IDC heart muscle. Native thick filaments from control myocardium were structurally stable. They had a median length of 1.65 μm with well-defined bare zones and displayed the 43 nm helical periodicity typical of the relaxed arrangement of myosin heads close to the filaments' shafts. In contrast, native IDC filaments were less stable, and had a median length of 0.9 μm. These filaments were highly disordered: they had no surface periodicity and myosin heads were positioned away from the filaments' shafts. The shorter, less stable, aperiodic thick filaments from IDC hearts appear to result from depletion of LC2 caused by increased activity of mekratin in the IDC myocardium.

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Authors and Affiliations

  1. Departments of Neurobiology and Anatomy, MCP Hahnernann School of Medicine, Allegheny University of the Health Sciences, Philadelphia, USA

    Rhea J.C. Levine

  2. Department of Pathology, University of Alabama at Birmingham, Birmingham, USA

    James B. Caulfield & Paul Norton

  3. Basic Veterinary Sciences, Royal Veterinary College, London, UK

    Peter D. Chantler

  4. Departments of Medicine and Biochemistry and Molecular Biology, Albany Medical College, Albany, USA

    Mark R. Deziel & Sarkis S. Margossian

  5. Dana-Farber Cancer Institute, Boston, USA

    Henry S. Slayter

Authors
  1. Rhea J.C. Levine
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  2. James B. Caulfield
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  3. Paul Norton
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  4. Peter D. Chantler
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  6. Henry S. Slayter
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  7. Sarkis S. Margossian
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Levine, R.J., Caulfield, J.B., Norton, P. et al. Myofibrillar protein structure and assembly during idiopathic dilated cardiomyopathy. Mol Cell Biochem 195, 1–10 (1999). https://doi.org/10.1023/A:1006940513097

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