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  • Polymer and Materials Science  (5)
  • Goats  (3)
  • Genetic diversity  (2)
  • Life Sciences (General)  (2)
  • Microsatellite  (2)
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  • 1
    Electronic Resource
    Electronic Resource
    Sequential pathological studies in the udder of goats intramammarily infected withAspergillus fumigatus (1994)
    Springer
    Mycopathologia 126 (1994), S. 9-14 
    Details
    ISSN: 1573-0832
    Keywords: Aspergillosis ; Goats ; Pathology ; Udder
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Intramammary inoculation of goats withAspergillus fumigatus spores resulted in the development of mastitis with characteristic gross and microscopic lesions. The mastitis and the lesions were restricted to the infected udder halves only and there was no dissemination of infection to other tissues of the body. The experiment was continued for 45 days. Gross changes in the infected udder were observed up to the 45th day post-infection. The lesions, in general, included variable sized abscesses in the first 15 days followed by development of varying sized greyish-white nodules in the infected udders. Microscopic changes consisted of granulomatous reaction with well developed granulomas in the infected udders. Hyphae and spores ofAspergillus fumigatus could be demonstrated in sections of the infected udders up to 45 days after infection. Reisolation of the fungus consistently was achieved up to 45 days. It is concluded that intramammary inoculation ofAspergillus fumigatus spores in goats leads to chronic granulomatous mastitis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01371167
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  • 2
    Electronic Resource
    Electronic Resource
    Sequential pathological studies in goats infected intratracheally withAspergillus fumigatus (1993)
    Springer
    Mycopathologia 121 (1993), S. 77-81 
    Details
    ISSN: 1573-0832
    Keywords: Aspergillosis ; Goats ; Pathology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Intratracheal inoculation of goats withAspergillus fumigatus spores resulted in the development of characteristic gross and microscopic lesions. The lesions were restricted to lungs and there was no dissemination of infection to other tissues of the body except liver in one goat 16 days after infection. The experiment was continued for 37 days. Gross changes in lungs were observed up to the 24th day post-infection. The lesions, in general, included congestion and oedema in the first 6 days followed by the development of varying greyish-white nodules in the lungs. Microscopic changes consisted of granulomatous reaction with well developed granulomas in lungs. Hyphae and conidiophores with fruiting bodies ofAspergillus fumigatus could be demonstrated in sections up to 24 days of infection. Reisolation of the fungus consistently was achieved up to 24 days. It is concluded that intratracheal inoculation ofAspergillus fumigatus spores in goats leads to pulmonary aspergillosis up to 24 days.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01103574
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  • 3
    Electronic Resource
    Electronic Resource
    Clinico-pathological studies on experimental cryptococcal mastitis in goats (1994)
    Springer
    Mycopathologia 126 (1994), S. 147-155 
    Details
    ISSN: 1573-0832
    Keywords: Cryptococcus neoformans ; Goats ; Mastitis ; Pathology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Unilateral intramammary inoculation of 10 goats withCryptococcus neoformans (2×106 yeast cells) resulted in the development of mastitis, with gross and microscopic lesions being restricted to the infected udder halves only and there was no dissemination of infection to the opposite uninfected udder halves as well as to other organs of the body. The experiment was continued for 40 days, with 2 animals each from the infected and control groups being killed on 5th, 10th, 20th, 30th and 40th day postinoculation (DPI). Initial enlargement of the infected udder halves was followed by marked decrease in size leading to very small, firm and nodular udder halves. After infection, there was also sharp fall in the milk yield. Cryptococcal organisms were demonstrated in the mastitic milk and udder impression smears with special stains.C. neoformans was reisolated from the milk of only infected udder halves up to 25th DPI. Microsopically, there was initially acute diffuse purulent mastitis which later on became chronic, characterised by marked infiltration of lymphocytes, macrophages, extensive fibrosis and development of multiple granulomas. The cryptococcal organisms could be demonstrated in the udder sections only up to 30th DPI. It is concluded that intramammary inoculation ofCryptococcus neoformans in goats leads to severe mastitis with sharp fall in milk yield.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01103768
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  • 4
    Electronic Resource
    Electronic Resource
    The use of microsatellites for detecting DNA polymorphism, genotype identification and genetic diversity in wheat (2000)
    Springer
    Theoretical and applied genetics 100 (2000), S. 584-592 
    Details
    ISSN: 1432-2242
    Keywords: Key words Microsatellite markers ; Wheat ; Genetic diversity ; Genotype identification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A set of 20 wheat microsatellite markers was used with 55 elite wheat genotypes to examine their utility (1) in detecting DNA polymorphism, (2)in the identifying genotypes and (3) in estimating genetic diversity among wheat genotypes. The 55 elite genotypes of wheat used in this study originated in 29 countries representing six continents. A total of 155 alleles were detected at 21 loci using the above microsatellite primer pairs (only 1 primer amplified 2 loci; all other primers amplified 1 locus each). Of the 20 primers amplifying 21 loci, 17 primers and their corresponding 18 loci were assigned to 13 different chromosomes (6 chromosomes of the A genome, 5 chromosomes of the B genome and 2 chromosomes of the D genome). The number of alleles per locus ranged from 1 to 13, with an average of 7.4 alleles per locus. The values of average polymorphic information content (PIC) and the marker index (MI) for these markers were estimated to be 0.71 and 0.70, respectively. The (GT)n microsatellites were found to be the most polymorphic. The genetic similarity (GS) coefficient for all possible 1485 pairs of genotypes ranged from 0.05 to 0.88 with an average of 0.23. The dendrogram, prepared on the basis of similarity matrix using the UPGMA algorithm, delineated the above genotypes into two major clusters (I and II), each with two subclusters (Ia, Ib and IIa, IIb). One of these subclusters (Ib) consisted of a solitary genotype (E3111) from Portugal, so that it was unique and diverse with respect to all other genotypes belonging to cluster I and placed in subcluster Ia. Using a set of only 12 primer pairs, we were able to distinguish a maximum of 48 of the above 55 wheat genotypes. The results demonstrate the utility of microsatellite markers for detecting polymorphism leading to genotype identification and for estimating genetic diversity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050077
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  • 5
    Electronic Resource
    Electronic Resource
    A microsatellite marker associated with a QTL for grain protein content on chromosome arm 2DL of bread wheat (1999)
    Springer
    Theoretical and applied genetics 99 (1999), S. 341-345 
    Details
    ISSN: 1432-2242
    Keywords: Key words Bread wheat ; Grain protein content ; Microsatellite ; STMS ; QTL analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  This study was undertaken with a view to tag gene(s) controlling grain protein content (GPC) using molecular markers in bread wheat. For this purpose, the genotype PH132 with high protein content (13.5%) was crossed with genotype WL711 with significantly lower protein content (9.7%), and 100 RILs were derived. These RILs showed normal distribution for protein content. The parental genotypes were analysed with 232 STMS primer pairs for detection of polymorphism. Of these, 167 primer pairs gave scorable amplification products, and 57 detected polymorphism between the parents. Using each of these 57 primer pairs, we carried out bulked segregant analysis on RILs representing the two extremes of the distribution. One primer pair for the locus wmc41 showed association with protein content. This was further confirmed through selective genotyping. The co-segregation data on the molecular marker (wmc41) and protein content on 100 RILs was analysed by means of a single-marker linear regression approach. Significant regression suggested linkage between wmc41 and a QTL (designated as QGpc.ccsu-2D.) for protein content. The results showed that this marker-linked QTL accounted for 18.73% of the variation for protein content between the parents. The marker has been located on chromosome arm 2DL using nulli-tetrasomic lines and two ditelocentric stocks for chromosome 2D.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051242
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  • 6
    Electronic Resource
    Electronic Resource
    Selection of diverse genotypes for heterosis in yield and response in toria (Brassica campestris L.) (1985)
    Springer
    Theoretical and applied genetics 69 (1985), S. 515-517 
    Details
    ISSN: 1432-2242
    Keywords: Toria ; Genetic diversity ; D2-correlation coefficient ; Heterosis ; Basic yield potential ; Brassica
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Thirty-one genotypes of toria (Brassica campestris L.) were grown in twelve environments and subsequently analysed in order to select potential parents which expressed diversity for both 12 different characters (estimated by Mahalanobis' D2 technique) and response to the environments (estimated on the basis of negative correlation between deviations in seed yield of a pair of genotypes from their respective environmental means). Coefficients of determination (r2) were also used to measure the reliability of correlation, which is the basis of diversity of response. Stability parameters (b and S2d) and mean seed yield were also considered in selecting potential parents. On the basis of these criteria, three pairs of genotypes (ITSA and TCSU-1, TCSU-7 and TH-8 and Ludhiana Composite-1 and TH-4) are recommended to be used as parents for hybridization programmes so that heterosis both in seed yield and response may be exploited.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00251095
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  • 7
    Electronic Resource
    Electronic Resource
    Identification of a microsatellite on chromosomes 6B and a STS on 7D of bread wheat showing an association with preharvest sprouting tolerance (1999)
    Springer
    Theoretical and applied genetics 99 (1999), S. 336-340 
    Details
    ISSN: 1432-2242
    Keywords: Key words Preharvest sprouting ; Microsatellite ; STMS ; STS ; Linkage ; Bread wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  In bread wheat, the transfer of tolerance to preharvest sprouting (PHS) that is associated with genotypes having red kernel colour to genotypes with amber kernels is difficult using conventional methods of plant breeding. The study here was undertaken to identify DNA markers linked with tolerance to PHS as these would allow indirect marker-assisted selection of PHS-tolerant genotypes with amber kernels. For this purpose, a set of 100 recombinant inbred lines (RILs) was developed using a cross between a PHS-tolerant genotype, SPR8198, with red kernels and a PHS-susceptible cultivar, ‘HD2329’, with white kernels. The two parents were analysed with 232 STMS (sequence-tagged microsatellite site) and 138 STS (sequence-tagged site) primer pairs. A total of 300 (167 STMSs and 133 STSs) primer pairs proved functional by giving scorable PCR products. Of these, 57 (34%) STMS and 30 (23%) STS primer pairs detected reproducible polymorphism between the parent genotypes. Using these primer pairs, we carried out bulked segregant analysis on two bulked DNAs, one obtained by pooling DNA from 5 PHS-tolerant RILs and the other similarly derived by pooling DNA from 5 PHS-susceptible RILs. Two molecular markers, 1 STMS primer pair for the locus wmc104 anda STS primer pair for the locus MST101, showed apparent linkage with tolerance to PHS. This was confirmed following selective genotyping of individual RILs included in the bulks. Chi-square contingency tests for independence were conducted on the cosegregation data collected on 100 RILs involving each of the two molecular markers (wmc104 and MST101) and PHS. The tests revealed a strong association between each of the markers and tolerance to PHS. Using nullisomic-tetrasomic lines, we were able to assign wmc104 and MST101 to chromosomes 6B and 7D, respectively. The results also indicated that the tolerance to PHS in SPR8198 is perhaps governed by two genes (linked with two molecular markers) exhibiting complementary interaction.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051241
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  • 8
    Electronic Resource
    Electronic Resource
    Particulate precipitation polymerization: A convenient procedure for the synthesis of crosslinked polymers useful as polymeric supports (1980)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 25 (1980), S. 2559-2566 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Suitable choice of monomer/nonsolvent and monomer plus solvent/nonsolvent ratios affords a very simple and convenient laboratory scale method for the synthesis of functionalized crosslinked polymers suitable as polymeric supports in a wide variety of applications. The method involves an initially homogeneous solution polymerization which, because of the presence of nonsolvents ultimately produces insoluble, particulate (i.e., nonswollen) resin. Advantages over the usual suspension polymerization processes include the absence of stabilizers and suspension agents and complete freedom to utilize water soluble or water insoluble monomers, or mixtures of the two. Reaction conditions must be determined experimentally for individual systems and a number of representative examples are given.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1980.070251112
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  • 9
    Electronic Resource
    Electronic Resource
    Molecular weight-intrinsic viscosity relationship of trans-16-hydroxy-9-enoate hexadecenoic acid polyesters (1986)
    Weinheim : Wiley-Blackwell
    Angewandte Makromolekulare Chemie 144 (1986), S. 167-173 
    Details
    ISSN: 0003-3146
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Description / Table of Contents: Die nichtkatalysierte Kinetik der Selbstveresterung von trans-16-Hydroxy-9-enoathexadecenoylsäure folgt einer Reaktion 3. Ordnung. Eine Beziehung zwischen der Grenzviskosität und dem Molekulargewicht ihrer Polyester wurde zu [η] = 14,12 · 10-5 M0,95 (25°C, Chloroform) bestimmt. Die Untersuchungen zur Polymolekularität der Polyester zeigten die übliche Form der Integral- und Differentialkurven.
    Notes: The uncatalysed kinetics of self-esterification of trans-16-hydroxy-9-enoate hexadecenoic acid has been found to follow third order. A relationship between intrinsic viscosity and molecular weight of its polyesters [η] = 14.2 · 10-5 M0.95 (25°C, chloroform) has been put forward. The polymolecularity studies of the polyester have shown usual characteristics of integral and differential curves.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/apmc.1986.051440114
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  • 10
    Electronic Resource
    Electronic Resource
    Fluoroalkylethyl silane coating as a moisture barrier for dental ceramics (1993)
    Hoboken, NJ : Wiley-Blackwell
    Journal of Biomedical Materials Research 27 (1993), S. 415-417 
    Details
    ISSN: 0021-9304
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: The performance of dental ceramics is reduced in wet environments due to stress-corrosion limiting restoration lifetime. As analysis suggests that fracture is typically initiated from the internal surface, placement of a thin coating before a restoration is cemented to place may prevent fracture if it acts as a moisture barrier. Of coating we tested, a fluoroalkylethyl silane (du Pont MPD-7680) showed promise. This study aimed to test a range of concentrations on glass and to determine any strength improvement on dental ceramics. Glass slides (n = 8-9) were indented (29-N load) stored 24 h, etched, coated with 100, 50, and 25% fluorosilane in isopropyl alcohol, and air-cured for 24 h. The slides were fractured on a biaxial fixture in water at 0.5 mm/min and maximum stresses calculated. Uncoated controls were tested in water and liquid N2 to determine the inert strength without stress-corrosion. Results showed increasing mean strength from uncoated (53.6 MPa) to 100% (68.0 MPa) which compared to the 80.4 MPa inert strength. ANOVA showed P 〈 .05. Subsequently the experiment was repeated on discs of three dental ceramics (n = 7-10): feldspathic (Ceramco), glass-ceramic (Dicor MGC), high-leucite (Optec) with the 100% solution only. Mean strength was significantly (P 〈 .01) enhanced in the feldspathic (56.2 MPa, untreated, 70.7 MPa, treated) but not the high-leucite (83.1, 83.0) or the glass-ceramic (283.9, 271.4). Fluoroalkylethyl silane coating reduced stres-corrosion in glass and feldspathic porcelain. It may be a practical approach to reducing fracture of ceramic restorations. © 1993 John Wiley & Sons, Inc.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jbm.820270315
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