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  • SSCP  (2)
  • Filled seeds  (1)
  • Genetic response  (1)
  • 1
    Electronic Resource
    Electronic Resource
    The beta-binomial model for estimating heritabilities of binary traits (1995)
    Springer
    Theoretical and applied genetics 91 (1995), S. 544-552 
    Details
    ISSN: 1432-2242
    Keywords: Simulation ; Genetic response ; Family mean heritability ; Sib analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Calculations of individual narrow-sense heritability and family mean heritability of a binary trait in stochastically simulated sib trials in completely randomized block experiments showed that in some situations estimates of “realized” heritabilities obtained from the mixed linear threshold model could be improved by application of a proposed beta-binomial model. The proposed model adopts the beta-binomial as the conjugate-prior for the distribution of probabilities of observing the binary trait in a genetic entry. Estimation of the beta parameters allows an estimation of selection response and, by linkage to a threshold model for the individual observations, the desired heritabilities can be obtained. The average bias in the betabinomial estimates of heritability and family mean heritability was less than 2%. Improvements over existing procedures were especially manifest at heritabilities above 0.3 and at low overall probabilities of observing the trait (p 〈 0.30). The lowest root mean square errors were consistently obtained with the algorithm proposed by Harville and Mee (1984). The beta-binomial framework, although restricted to a single random additive genetic effect, further facilitates general analysis, estimation of selection response, and calculation of reliable family mean heritability. Intraclass correlations can be estimated directly from the beta-binomial parameters.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222986
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  • 2
    Electronic Resource
    Electronic Resource
    Inheritance and molecular variations of PCR-SSCP fragments in pedunculate oak (Quercus robur L.) (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 348-354 
    Details
    ISSN: 1432-2242
    Keywords: Quercus robur ; PCR ; SSCP ; Co-dominant marker ; Mendelian inheritance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Single-strand conformaiton polymorphism (SSCP) profiles of six PCR-amplified fragments (250–800 bp) were analyzed in three full-sib families of pedunculate oak (Quercus robur L.) and their parents. Among the six fragments, four were polymorphic and one exhibited complex patterns that were not changed by varying the SSCP conditions. The number of bands for the analyzed fragments varied between two and four among individuals regardless of fragment size. As shown by segregation data, the variation in the number of bands between trees could only be attributed to the allelic composition (homozygotes vs heterozygotes): a genotype that exhibited two bands was presumptively homozygous, wheras a genotype exhibiting three or four bands was heterozygous. Mendelian proportions were observed in all crosses for each polymorphic fragment. In one cross, we could clearly identify a null allele due to a possible mutation at a primer site. Single-base mutations and short insertion-deletions were shown to be the molecular causes of the SSCP polymorphism observed between different alleles. The use of SSCP as a technique to identify co-dominant markers of PCR fragments (up to 800 bp) is recommended for gene diversity studies or for gene mapping.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223175
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  • 3
    Electronic Resource
    Electronic Resource
    Relationship between inbreeding depression and inbreeding coefficient in maritime pine (Pinus pinaster) (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 347-356 
    Details
    ISSN: 1432-2242
    Keywords: Pinus pinaster ; Inbreeding depression ; Filled seeds ; Adult growth ; Genetic load
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The relationship between inbreeding depression and inbreeding coefficient (F) for several important traits was investigated in an 11-year trial of maritime pine (Pinus pinaster). Five levels of inbreeding (F=0; 0.125; 0.25; 0.5; 0.75) were obtained in a mating design involving ten plus-trees, or their progenies, as parents (total of 51 families). For F=0.75, the mean inbreeding depressions were 27% for height, 37% for circumference at breast height (63% for bole volume), 23% for basal straightness (better straightness of the inbred trees), and 89% for female fertility (number of cones). Large differences were observed among inbred families for the same level of inbreeding. The evolution of depression with F was more or less linear, depending on the traits. Significant differences among F-levels appeared very early for height (from 5-years of age). Inbreeding depression was much more expressed during unfavorable years than during favorable years for yearly height growth. When compared with other Pinus species, maritime pine appears to be less affected by inbreeding, especially for the percentage of filled seeds and general vigor. A reduced genetic load in maritime pine may result from the evolutionary history of the species and its scattered distribution.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223678
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  • 4
    Electronic Resource
    Electronic Resource
    Developing SSCP markers in two Pinus species (1999)
    Springer
    Molecular breeding 5 (1999), S. 21-31 
    Details
    ISSN: 1572-9788
    Keywords: SSCP ; Pinus pinaster ; Pinus sylvestris ; linkage map
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract This study demonstrates the feasibility of generating sequence- based markers in Pinus species, from data available in electronic databases. Nucleotide sequences from 23 partially or fully characterized cDNAs or genomic sequences of pines were used to design PCR primers for amplifying targeted fragments of genomic DNA from Maritime and Scots pine. Various template DNA and MgCl2 concentrations, annealing temperatures, and buffer compositions were used to optimize the PCR amplifications. The polymorphism of 16 sequences was then investigated in a tree-generation inbred pedigree of Maritime pine and in a two-generation pedigree of Scots pine, using single-stranded DNA conformation polymorphism (SSCP) on polymerase chain reaction (PCR) products. The level of polymorphism was shown to be independent of (1) fragment size, (2) the presence or absence of introns in the amplified product and (3) temperature during electrophoresis. Mendelian segregation was tested for 5 SSCP markers in each species. Chromosomal locations of five genes were identified by linkage analysis with previously mapped markers in a genetic map of Maritime pine. The use of SSCP is recommended for constructing a transcriptional map for comparative mapping studies among pines and to provide useful ‘candidate genes’ for characterizing quantitative trait loci.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009635226412
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