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  • Cell & Developmental Biology  (2)
  • Cytochemistry  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Cytochemistry and freeze-fracture of membranes isolated from the electrocyte of Electrophorus electricus (L.) (1979)
    Springer
    Cell & tissue research 202 (1979), S. 275-281 
    Details
    ISSN: 1432-0878
    Keywords: Plasma membrane ; Cytochemistry ; Anionic sites ; Freeze-fracture ; Electrophorus electricus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Membranes were isolated from the main electric organ of Electrophorus electricus and studied by means of cytochemistry and freezefracture. The membrane fractions consisted of vesicles inside-in as determined by localization of anionic sites using colloidal iron and cationized ferritin particles. The anionic sites were not homogeneously distributed on the surface of the vesicle. Freeze-fracture showed the presence of intramembranous particles associated with either protoplasmic (P) or extracellular (E) faces of the membrane. Regions of the membrane without particles were observed. The results are discussed in relation to the existence of association between intramembranous particles and membrane receptors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232241
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  • 2
    Electronic Resource
    Electronic Resource
    Rapid primary microwave-glutaraldehyde fixation preserves the plasma membrane and intracellular structures of the protozoan Tritrichomonas foetus (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    Microscopy Research and Technique 25 (1993), S. 286-290 
    Details
    ISSN: 1059-910X
    Keywords: Microwave fixation ; Freeze-fracture ; Electron microscopy ; Protozoan ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: Tritrichomonas foetus, a pathogenic protozoan, was used as a model to analyse microwave-stimulated fixation as a procedure of preparation of biological samples for electron microscopy of thin sections and freeze-fracture replicas. Good preservation of the protozoan structure was achieved by microwave-stimulated fixation and Epon polymerization. The membrane structure, as visualized in freeze-fracture replicas, was well preserved. © 1993 Wiley-Liss, Inc.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1070250404
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  • 3
    Electronic Resource
    Electronic Resource
    Fine structure of Tritrichomonas foetus as seen using cryotechniques (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    Microscopy Research and Technique 29 (1994), S. 37-46 
    Details
    ISSN: 1059-910X
    Keywords: Tritrichomonas foetus ; Freeze-fracture ; Electron microscopy ; Fast-freezing fixation ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: Tritrichomonas foetus was studied using different physical and chemical fixation methods such as fast-freezing (by high pressure, “slam-freezing,” and jet-propane), freeze-substitution, conventional freeze-fracture and deep-etching, cryoultramicrotomy, and routine preparation for transmission electron microscopy. The use of fast-freezing fixation (FFF) proved to be superior in terms of structural preservation due to the rapidity of this fixation compared to that obtained using conventional chemical fixation. The low temperature techniques used here were useful to confirm data already obtained by conventional freeze-fracture using chemical fixation and cryoprotection, such as the presence of flagellar rosettes and costa structure. Cryoultramicrotomy and slam-freezing also demonstrated the presence of hair-like structures projecting out from the protozoan surface. New aspects of organelles of T. foetus were demonstrated. Published 1994 by Wiley-Liss, Inc.
    Additional Material: 21 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1070290106
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