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  • 1
    Electronic Resource
    Electronic Resource
    Changes in lipid composition during starvation and germ-tube formation inCandida albicans
    Amsterdam : Elsevier
    Experimental Mycology 5 (1981), S. 140-147 
    Details
    ISSN: 0147-5975
    Keywords: Candida albicans ; germ tubes ; germination ; lipids ; phospholipids ; sterols
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0147-5975(81)90014-1
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  • 2
    Electronic Resource
    Electronic Resource
    The alternate respiratory pathway of Candida albicans (1978)
    Springer
    Archives of microbiology 116 (1978), S. 61-67 
    Details
    ISSN: 1432-072X
    Keywords: Respiration ; Alternate oxidase ; Cytochrome c oxidase ; Candida albicans ; Cyanide and antimycin A insensitive respiration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Candida albicans contains a cryptic cyanide and antimycin A insensitive respiratory system. This alternate oxidase was found (i) at all growth rates from μ=0.05 to 0.26 in a chemostat culture and (ii) in both mycelial and yeast forms of the organism. Neither chloramphenicol nor cycloheximide prevented the expression of the alternate oxidase. Salicyl-hydroxamic acid was a potent inhibitor of the cyanide insensitive respiration. The respiration of mitochondria grown in the presence of antimycin A was not inhibited by cyanide or antimycin A but was inhibited by salicylhydroxamic acid.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00408734
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  • 3
    Electronic Resource
    Electronic Resource
    Regulation of chitin synthesis during germ-tube formation in Candida albicans (1980)
    Springer
    Archives of microbiology 125 (1980), S. 97-104 
    Details
    ISSN: 1432-072X
    Keywords: l-glutamine-d-fructose-6-phosphate aminotransferase ; Chitin synthase ; Chitin ; Candida albicans ; Germ-tube formation ; Dimorphism ; Polyoxin D
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The synthesis of chitin during germ-tube formation in Candida albicans may be regulated by the first and last steps in the chitin pathway: namely l-glutamine-d-fructose-6-phosphate aminotransferase and chitin synthase. Induction of germ-tube formation with either glucose and glutamine or serum was accompanied by a 4-fold increase in the specific activity of the aminotransferase. Chitin synthase in C. albicans is synthesized as a proenzyme. N-acetyl glucosamine increased the enzymic activity of the activated enzyme 3-fold and the enzyme exhibited positive co-operativity with the substrate, UDP-N-acetylglucosamine. Although chitin synthase was inhibited by polyoxin D (K i =1.2μM) this antibiotic did not affect germination. During germ-tube formation the total chitin synthase activity increased 1.4-fold and the expressed activity (in vivo activated proenzyme) increased 5-fold. These results could account for the reported 5-fold increase in chitin content observed during the yeast to mycelial transformation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00403204
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  • 4
    Electronic Resource
    Electronic Resource
    The Effect of Cetylpyridinium Chloride (CPC) on the Cell Surface Hydrophobicity and Adherence of Candida albicansto Human Buccal Epithelial Cells in Vitro (1995)
    Springer
    Pharmaceutical research 12 (1995), S. 1896-1900 
    Details
    ISSN: 1573-904X
    Keywords: Candida albicans ; cetylpyridinium chloride ; reduced adherence ; reduced cell surface hydrophobicity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Purpose. This study examined the effects of cetylpyridinium chloride (CPC) on cell surface hydrophobicity (CSH) and adherence of blastospores of Candida albicans(MEN strain) to human buccal epithelial cells (EEC) in vitro. Methods. The effect of CPC treatment of either C. albicans blastospores or BEC on their subsequent adherence was determined using 35SO4 labelled blastospores in association with a Percoll™ gradient. The effects of CPC treatment of blastospores on their CSH was determined using Hydrophobic Interaction Chromatography. Results. Treatment of exponential and stationary phase blastospores with CPC (50 µg mL−1) for 0.5–30 minutes, or with CPC (0.5–50 µg mL−1) for 15 minutes resulted in significant reductions in both blastospore CSH and adherence to BEC in vitro. No correlation was apparent (r 〈 0.8) between reduced CSH and reduced blastospore adherence following treatment with CPC (0.5–50 µg mL−1). Significantly reduced adherence of C. albicans (stationary or exponential growth phases) to human EEC was also observed following treatment of BEC with CPC (50 µg mL−1) for 0.5–30 minutes or with CPC (0.5–50 µg mL−1) for 15 minutes. Antiadherence effects were observed at both sub and super-minimum inhibitory concentrations of CPC. Conclusions. It is suggested that, whilst the ability of CPC to reduce the CSH of C. albicans may contribute to its reduced adherence to human BEC in vitro, reduced CSH is only one of several possible factors that contribute to the observed antiadherence effects.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1016231620470
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  • 5
    Electronic Resource
    Electronic Resource
    Cloning and expression of Candida albicans ADE2 and proteinase genes on a replicative plasmid in C. albicans and in Saccharomyces cerevisiae (1992)
    Springer
    Molecular genetics and genomics 235 (1992), S. 453-457 
    Details
    ISSN: 1617-4623
    Keywords: Autonomously replicating plasmid ; Proteinase ; Candida albicans
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A plasmid vector (denoted pRC2312) was constructed, which replicates autonomously in Escherichia coli, Saccharomyces cerevisiae and Candida albicans. It contains LEU2, URA3 and an autonomously replicating sequence (ARS) from C. albicans for selection and replication in yeasts, and bla (ampicillin resistance) and ori for selection and replication in E. coli. S. cerevisiae AH22 (Leu−) was transformed by pRC2312 to Leu− at a frequency of 1.41 × 105 colonies per μg DNA. Transformation of C. albicans SGY-243 (Ura-) to Ura+ with pRC2312 resulted in smaller transformant colonies at a frequency of 5.42 × 103 per μg DNA where the plasmid replicated autonomously in transformed cells, and larger transformant colonies at a frequency of 32 per μg DNA, in which plasmid integrated into the genome. Plasmid copy number in yeasts was determined by a DNA hybridization method and was estimated to be 15±3 per haploid genome in S. cerevisiae and 2–3 per genome in C. albicans replicative transformants. Multiple tandem integration occurred in integrative transformants and copy number of the integrated sequence was estimated to be 7–12 per diploid genome. The C. albicans ADE2 gene was ligated into plasmid pRC2312 and the construct transformed Ade− strains of both C. albicans and S. cerevisiae to Ade+. The vector pRC2312 was also used to clone a fragment of C. albicans genomic DNA containing an aspartic proteinase gene. C. albicans transformants harboring this plasmid showed a two-fold increase in aspartic proteinase activity. However S. cerevisiae transformants showed no such increase in proteinase activity, suggesting the gene was not expressed in S. cerevisiae.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00279393
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  • 6
    Electronic Resource
    Electronic Resource
    Isolation and nucleotide sequence of an autonomously replicating sequence (ARS) element functional in Candida albicans and Saccharomyces cerevisiae (1990)
    Springer
    Molecular genetics and genomics 221 (1990), S. 210-218 
    Details
    ISSN: 1617-4623
    Keywords: Candida albicans ; Autonomously replicating sequence (ARS) ; 5S rRNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary An 8.6-kb fragment was isolated from an EcoRI digest of Candida albicans ATCC 10261 genomic DNA which conferred the property of autonomous replication in Saccharomyces cervisiae on the otherwise non-replicative plasmid pMK155 (5.6 kb). The DNA responsible for the replicative function was subcloned as a 1.2-kb fragment onto a non-replicative plasmid (pRC3915) containing the C. albicans URA3 and LEU2 genes to form plasmid pRC3920. This plasmid was capable of autonomous replication in both S. cerevisiae and C. albicans and transformed S. cerevisiae AH22 (leu2 −) to Leu+ at a frequency of 2.15 × 103 transformants per pg DNA, and transformed C. albicans SGY-243 (Δura3) to Ura+ at a frequency of 1.91 × 103 transformants per μg DNA. Sequence analysis of the cloned DNA revealed the presence of two identical regions of eleven base pairs (5′TTTTATGTTTT3′) which agreed with the consensus of autonomously replicating sequence (ARS) cores functional in S. cerevisiae. In addition there were two 10/11 and numerous 9/11 matches to the core consensus. The two 11/11 matches to the consensus, CaARS1 and CaARS2, were located on opposite strands in a non-coding AT-rich region and were separated by 107 bp. Also present on the C. albicans DNA, 538 by from the ARS cores, was a gene for 5S rRNA which showed sequence homology with several other yeast 5S rRNA genes. A sub-fragment (494 bp) containing the 5S rRNA gene (but not the region containing the ARS cores) hybridized to genomic DNAs from a number of yeast species, including S. cerevisiae, C. tropicalis, C. pseudotropicalis, C. parapsilosis, C. kruseii, C. (Torulopsis) glabrata and Neurospora crassa. The 709-bp ARS element (but not the 5S rRNA gene) was necessary for high-frequency transformation and autonomous plasmid replication in both S. cerevisiae and C. albicans.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00261723
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  • 7
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    LHC bounds on interactions of dark matter (2011)
    American Physical Society (APS)
    Details
    Publication Date: 2011-11-10
    Description: Author(s): Arvind Rajaraman, William Shepherd, Tim M. P. Tait, and Alexander M. Wijangco [Phys. Rev. D 84, 095013] Published Wed Nov 09, 2011
    Keywords: Beyond the standard model
    Print ISSN: 0556-2821
    Electronic ISSN: 1089-4918
    Topics: Physics
    Published by American Physical Society (APS)
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  • 8
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    Interference effects in heavy W^{′}-boson searches at the LHC (2012)
    American Physical Society (APS)
    Details
    Publication Date: 2012-06-23
    Description: Author(s): Elena Accomando, Diego Becciolini, Stefania De Curtis, Daniele Dominici, Luca Fedeli, and Claire Shepherd-Themistocleous Interference effects are widely neglected in searches for new physics. This is the case in recent publications on searches for W ′ bosons using leptonic final states. We examine the effects of interference on distributions frequently used to determine mass limits for possible W ′ bosons and show that ... [Phys. Rev. D 85, 115017] Published Fri Jun 22, 2012
    Keywords: Beyond the standard model
    Print ISSN: 0556-2821
    Electronic ISSN: 1089-4918
    Topics: Physics
    Published by American Physical Society (APS)
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  • 9
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    Simplified model approach to same-sign dilepton resonances (2011)
    American Physical Society (APS)
    Details
    Publication Date: 2011-08-06
    Description: Author(s): Vikram Rentala, William Shepherd, and Shufang Su We discuss same-sign dilepton resonances in the simplified model approach. The relevant SU(3) Q J quantum numbers are 1 2 0,1,2 . For simplicity, we only consider a spin 0 scalar, which is typically referred to as a doubly charged Higgs in the literature. We consider the three simplest cases where the do... [Phys. Rev. D 84, 035004] Published Fri Aug 05, 2011
    Keywords: Beyond the standard model
    Print ISSN: 0556-2821
    Electronic ISSN: 1089-4918
    Topics: Physics
    Published by American Physical Society (APS)
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  • 10
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    Cascading effects of the loss of apex predatory sharks from a coastal ocean (2007)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 2007-03-31
    Description: Impacts of chronic overfishing are evident in population depletions worldwide, yet indirect ecosystem effects induced by predator removal from oceanic food webs remain unpredictable. As abundances of all 11 great sharks that consume other elasmobranchs (rays, skates, and small sharks) fell over the past 35 years, 12 of 14 of these prey species increased in coastal northwest Atlantic ecosystems. Effects of this community restructuring have cascaded downward from the cownose ray, whose enhanced predation on its bay scallop prey was sufficient to terminate a century-long scallop fishery. Analogous top-down effects may be a predictable consequence of eliminating entire functional groups of predators.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Myers, Ransom A -- Baum, Julia K -- Shepherd, Travis D -- Powers, Sean P -- Peterson, Charles H -- New York, N.Y. -- Science. 2007 Mar 30;315(5820):1846-50.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biology, Dalhousie University, 1355 Oxford Street, Halifax, NS B3H 4J1, Canada.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/17395829" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Atlantic Ocean ; Bivalvia ; Conservation of Natural Resources ; *Ecosystem ; *Elasmobranchii ; Fisheries ; *Food Chain ; Ostreidae ; Population Dynamics ; Population Growth ; Predatory Behavior ; *Sharks ; Skates (Fish)
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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