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  • Polymer and Materials Science  (5)
  • Analytical Chemistry and Spectroscopy  (4)
  • Aspergillosis  (2)
  • Genetic diversity  (2)
  • Life Sciences (General)  (2)
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  • 1
    Electronic Resource
    Electronic Resource
    Sequential pathological studies in the udder of goats intramammarily infected withAspergillus fumigatus (1994)
    Springer
    Mycopathologia 126 (1994), S. 9-14 
    Details
    ISSN: 1573-0832
    Keywords: Aspergillosis ; Goats ; Pathology ; Udder
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Intramammary inoculation of goats withAspergillus fumigatus spores resulted in the development of mastitis with characteristic gross and microscopic lesions. The mastitis and the lesions were restricted to the infected udder halves only and there was no dissemination of infection to other tissues of the body. The experiment was continued for 45 days. Gross changes in the infected udder were observed up to the 45th day post-infection. The lesions, in general, included variable sized abscesses in the first 15 days followed by development of varying sized greyish-white nodules in the infected udders. Microscopic changes consisted of granulomatous reaction with well developed granulomas in the infected udders. Hyphae and spores ofAspergillus fumigatus could be demonstrated in sections of the infected udders up to 45 days after infection. Reisolation of the fungus consistently was achieved up to 45 days. It is concluded that intramammary inoculation ofAspergillus fumigatus spores in goats leads to chronic granulomatous mastitis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01371167
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  • 2
    Electronic Resource
    Electronic Resource
    Sequential pathological studies in goats infected intratracheally withAspergillus fumigatus (1993)
    Springer
    Mycopathologia 121 (1993), S. 77-81 
    Details
    ISSN: 1573-0832
    Keywords: Aspergillosis ; Goats ; Pathology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Intratracheal inoculation of goats withAspergillus fumigatus spores resulted in the development of characteristic gross and microscopic lesions. The lesions were restricted to lungs and there was no dissemination of infection to other tissues of the body except liver in one goat 16 days after infection. The experiment was continued for 37 days. Gross changes in lungs were observed up to the 24th day post-infection. The lesions, in general, included congestion and oedema in the first 6 days followed by the development of varying greyish-white nodules in the lungs. Microscopic changes consisted of granulomatous reaction with well developed granulomas in lungs. Hyphae and conidiophores with fruiting bodies ofAspergillus fumigatus could be demonstrated in sections up to 24 days of infection. Reisolation of the fungus consistently was achieved up to 24 days. It is concluded that intratracheal inoculation ofAspergillus fumigatus spores in goats leads to pulmonary aspergillosis up to 24 days.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01103574
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  • 3
    Electronic Resource
    Electronic Resource
    The use of microsatellites for detecting DNA polymorphism, genotype identification and genetic diversity in wheat (2000)
    Springer
    Theoretical and applied genetics 100 (2000), S. 584-592 
    Details
    ISSN: 1432-2242
    Keywords: Key words Microsatellite markers ; Wheat ; Genetic diversity ; Genotype identification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A set of 20 wheat microsatellite markers was used with 55 elite wheat genotypes to examine their utility (1) in detecting DNA polymorphism, (2)in the identifying genotypes and (3) in estimating genetic diversity among wheat genotypes. The 55 elite genotypes of wheat used in this study originated in 29 countries representing six continents. A total of 155 alleles were detected at 21 loci using the above microsatellite primer pairs (only 1 primer amplified 2 loci; all other primers amplified 1 locus each). Of the 20 primers amplifying 21 loci, 17 primers and their corresponding 18 loci were assigned to 13 different chromosomes (6 chromosomes of the A genome, 5 chromosomes of the B genome and 2 chromosomes of the D genome). The number of alleles per locus ranged from 1 to 13, with an average of 7.4 alleles per locus. The values of average polymorphic information content (PIC) and the marker index (MI) for these markers were estimated to be 0.71 and 0.70, respectively. The (GT)n microsatellites were found to be the most polymorphic. The genetic similarity (GS) coefficient for all possible 1485 pairs of genotypes ranged from 0.05 to 0.88 with an average of 0.23. The dendrogram, prepared on the basis of similarity matrix using the UPGMA algorithm, delineated the above genotypes into two major clusters (I and II), each with two subclusters (Ia, Ib and IIa, IIb). One of these subclusters (Ib) consisted of a solitary genotype (E3111) from Portugal, so that it was unique and diverse with respect to all other genotypes belonging to cluster I and placed in subcluster Ia. Using a set of only 12 primer pairs, we were able to distinguish a maximum of 48 of the above 55 wheat genotypes. The results demonstrate the utility of microsatellite markers for detecting polymorphism leading to genotype identification and for estimating genetic diversity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050077
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  • 4
    Electronic Resource
    Electronic Resource
    Selection of diverse genotypes for heterosis in yield and response in toria (Brassica campestris L.) (1985)
    Springer
    Theoretical and applied genetics 69 (1985), S. 515-517 
    Details
    ISSN: 1432-2242
    Keywords: Toria ; Genetic diversity ; D2-correlation coefficient ; Heterosis ; Basic yield potential ; Brassica
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Thirty-one genotypes of toria (Brassica campestris L.) were grown in twelve environments and subsequently analysed in order to select potential parents which expressed diversity for both 12 different characters (estimated by Mahalanobis' D2 technique) and response to the environments (estimated on the basis of negative correlation between deviations in seed yield of a pair of genotypes from their respective environmental means). Coefficients of determination (r2) were also used to measure the reliability of correlation, which is the basis of diversity of response. Stability parameters (b and S2d) and mean seed yield were also considered in selecting potential parents. On the basis of these criteria, three pairs of genotypes (ITSA and TCSU-1, TCSU-7 and TH-8 and Ludhiana Composite-1 and TH-4) are recommended to be used as parents for hybridization programmes so that heterosis both in seed yield and response may be exploited.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00251095
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  • 5
    Electronic Resource
    Electronic Resource
    Determination of the isomeric distribution and associated impurities in commercial diethylbenzenes by capillary gas chromatography (1980)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 3 (1980), S. 497-502 
    Details
    ISSN: 0935-6304
    Keywords: Capillary gas chromatography ; Diethylbenzenes analysis, C10 aromatics analysis ; Retention indices ; Carbowax-1540 ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A capillary gas chromatographic method for the quantitative analysis of diethylbenzene fractions is described. Estimation of ortho-, meta- and para-isomers and other C9 and C10 aromatic impurities is covered. The conditions developed involve the use of a capillary column of Carbowax-1540 (300 feet × 0.01 inch) under isothermal operation. The retention index data for a number of aromatics are presented at four temperatures (90, 100, 110 and 120°C). The method offers a good choice for any level of concentration both for isomers and impurities commonly encountered within a reasonable analysis time.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240031003
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  • 6
    Electronic Resource
    Electronic Resource
    Determination of UV-transparent compounds by liquid chromatography using the UV detector (1986)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 9 (1986), S. 572-576 
    Details
    ISSN: 0935-6304
    Keywords: Liquid chromatography, LC ; Chromophore mobile phase ; Indirect UV detection ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: An indirect UV photometric detection technique is described in which a low concentration of a UV-absorbing compound (UVAC) is added to the mobile phase in reversed phase liquid chromatography, thereby making it possible for non UV-absorbing compounds such as the lower alcohols to be detected by the UV detector. This happens because the injected analyte may extract a portion of the UV absorbing compound from the mobile and/or stationary phase and the complex is co-eluted as a positive peak at the retention time of the analyte. Alternatively, the injected analyte may appear as a negative peak if the UV-absorbing compound is transferred to the mobile and stationary phases. In any case, the injected compound appears either as a positive or negative peak depending on the relative polarities and concentrations of all the compounds in the system. In addition, the resulting excess or deficiency of detection agent in the stationary phase is eluted separately as a positive or negative peak, indicating that the system has returned to equilibrium. In the work described herein, the chromatographic conditions and variables of the indirect photometric technique were studied to develop a quantitative HPLC method for UV-transparent compounds. It was found that under optimal conditions it is possible to determine some analytes quantitatively at concentrations as low as 0.05%.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240091006
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  • 7
    Electronic Resource
    Electronic Resource
    Pneumatic pulse dampener for liquid chromatography (1985)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 8 (1985), S. 177-180 
    Details
    ISSN: 0935-6304
    Keywords: Liquid chromatography, LC ; Pulse dampener, pneumatic ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A simple pulse dampener is described for use with reciprocating pumps. The dampener may be assembled locally form readily available components and consists mainly of a small reservoir of compressed helium which acts as a cushion to dampen the mobile phase pulsations pneumatically. Depulsing is 99% complete and is uniform through a wide pressure range. The pneumatic dampener allows the use of single- piston reciprocating pumps in conjunction with highly flow-sensitive detectors at their highest sensitivity settings.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240080404
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  • 8
    Electronic Resource
    Electronic Resource
    Particulate precipitation polymerization: A convenient procedure for the synthesis of crosslinked polymers useful as polymeric supports (1980)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 25 (1980), S. 2559-2566 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Suitable choice of monomer/nonsolvent and monomer plus solvent/nonsolvent ratios affords a very simple and convenient laboratory scale method for the synthesis of functionalized crosslinked polymers suitable as polymeric supports in a wide variety of applications. The method involves an initially homogeneous solution polymerization which, because of the presence of nonsolvents ultimately produces insoluble, particulate (i.e., nonswollen) resin. Advantages over the usual suspension polymerization processes include the absence of stabilizers and suspension agents and complete freedom to utilize water soluble or water insoluble monomers, or mixtures of the two. Reaction conditions must be determined experimentally for individual systems and a number of representative examples are given.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1980.070251112
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  • 9
    Electronic Resource
    Electronic Resource
    Molecular weight-intrinsic viscosity relationship of trans-16-hydroxy-9-enoate hexadecenoic acid polyesters (1986)
    Weinheim : Wiley-Blackwell
    Angewandte Makromolekulare Chemie 144 (1986), S. 167-173 
    Details
    ISSN: 0003-3146
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Description / Table of Contents: Die nichtkatalysierte Kinetik der Selbstveresterung von trans-16-Hydroxy-9-enoathexadecenoylsäure folgt einer Reaktion 3. Ordnung. Eine Beziehung zwischen der Grenzviskosität und dem Molekulargewicht ihrer Polyester wurde zu [η] = 14,12 · 10-5 M0,95 (25°C, Chloroform) bestimmt. Die Untersuchungen zur Polymolekularität der Polyester zeigten die übliche Form der Integral- und Differentialkurven.
    Notes: The uncatalysed kinetics of self-esterification of trans-16-hydroxy-9-enoate hexadecenoic acid has been found to follow third order. A relationship between intrinsic viscosity and molecular weight of its polyesters [η] = 14.2 · 10-5 M0.95 (25°C, chloroform) has been put forward. The polymolecularity studies of the polyester have shown usual characteristics of integral and differential curves.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/apmc.1986.051440114
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  • 10
    Electronic Resource
    Electronic Resource
    Fluoroalkylethyl silane coating as a moisture barrier for dental ceramics (1993)
    Hoboken, NJ : Wiley-Blackwell
    Journal of Biomedical Materials Research 27 (1993), S. 415-417 
    Details
    ISSN: 0021-9304
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: The performance of dental ceramics is reduced in wet environments due to stress-corrosion limiting restoration lifetime. As analysis suggests that fracture is typically initiated from the internal surface, placement of a thin coating before a restoration is cemented to place may prevent fracture if it acts as a moisture barrier. Of coating we tested, a fluoroalkylethyl silane (du Pont MPD-7680) showed promise. This study aimed to test a range of concentrations on glass and to determine any strength improvement on dental ceramics. Glass slides (n = 8-9) were indented (29-N load) stored 24 h, etched, coated with 100, 50, and 25% fluorosilane in isopropyl alcohol, and air-cured for 24 h. The slides were fractured on a biaxial fixture in water at 0.5 mm/min and maximum stresses calculated. Uncoated controls were tested in water and liquid N2 to determine the inert strength without stress-corrosion. Results showed increasing mean strength from uncoated (53.6 MPa) to 100% (68.0 MPa) which compared to the 80.4 MPa inert strength. ANOVA showed P 〈 .05. Subsequently the experiment was repeated on discs of three dental ceramics (n = 7-10): feldspathic (Ceramco), glass-ceramic (Dicor MGC), high-leucite (Optec) with the 100% solution only. Mean strength was significantly (P 〈 .01) enhanced in the feldspathic (56.2 MPa, untreated, 70.7 MPa, treated) but not the high-leucite (83.1, 83.0) or the glass-ceramic (283.9, 271.4). Fluoroalkylethyl silane coating reduced stres-corrosion in glass and feldspathic porcelain. It may be a practical approach to reducing fracture of ceramic restorations. © 1993 John Wiley & Sons, Inc.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jbm.820270315
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