Publication Date:
2001-08-04
Description:
The transcription factor Twist initiates Drosophila mesoderm development, resulting in the formation of heart, somatic muscle, and other cell types. Using a Drosophila embryo sorter, we isolated enough homozygous twist mutant embryos to perform DNA microarray experiments. Transcription profiles of twist loss-of-function embryos, embryos with ubiquitous twist expression, and wild-type embryos were compared at different developmental stages. The results implicate hundreds of genes, many with vertebrate homologs, in stage-specific processes in mesoderm development. One such gene, gleeful, related to the vertebrate Gli genes, is essential for somatic muscle development and sufficient to cause neural cells to express a muscle marker.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Furlong, E E -- Andersen, E C -- Null, B -- White, K P -- Scott, M P -- K22 HG00045-02/HG/NHGRI NIH HHS/ -- Howard Hughes Medical Institute/ -- New York, N.Y. -- Science. 2001 Aug 31;293(5535):1629-33. Epub 2001 Aug 2.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Developmental Biology, Howard Hughes Medical Institute, Beckman Center B300, Stanford University School of Medicine, Stanford, CA 94305-5329, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11486054" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Drosophila/*embryology/genetics
;
*Drosophila Proteins
;
Ectoderm/cytology
;
Embryo, Nonmammalian/metabolism
;
Embryonic Development
;
*Gene Expression Profiling
;
*Gene Expression Regulation, Developmental
;
Genes, Insect
;
In Situ Hybridization
;
Insect Proteins/genetics
;
Membrane Glycoproteins/genetics
;
Mesoderm/cytology/*metabolism
;
Mutation
;
Nuclear Proteins/*genetics/physiology
;
Oligonucleotide Array Sequence Analysis
;
*Receptors, Cell Surface
;
Toll-Like Receptors
;
*Transcription Factors
;
Transcription, Genetic
;
Twist Transcription Factor
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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